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1.
Ann Ig ; 35(6): 707-714, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37476887

RESUMO

Abstract: The Erice 58 Charter titled "The Health of Migrants: a Challenge of Equity for the Public Health System", was unanimously approved at the conclusion of the 58th Residential Course of the School of Epidemiology and Preventive Medicine 'Giuseppe D'Alessandro' entitled "The Health of Migrants: a Challenge of Equity for the Public Health System. Epidemiological, clinical-relational, regulatory, organisational, training and public communication aspects at international, national and local level', which took place from 28 March to 2 April 2022 in Erice (Sicily, Italy), at the 'Ettore Majorana' Foundation and Centre for Scientific Culture. The Course was promoted by the Italian Society of Migration Medicine (S.I.M.M.) and the Italian Society of Hygiene, Preventive Medicine and Public Health (SItI), with the collaboration and patronage of the Istituto Superiore di Sanità (ISS). 72 learners participated (mainly resident doctors in 'Hygiene and Preventive Medicine' but also other health service professionals), whose average age was 37 years; on the basis of territorial origin, 13 of the 20 Italian regions were represented. During the intense learning experience, which consisted of 18 frontal lessons (with 20 lecturers from the bio-medical, socio-anthropological and journalistic fields) and 7 working group sessions (supported by 4 classroom tutors in addition to the lecturers) in 'blended learning' mode, the various dimensions and critical issues related to the possibility of guaranteeing truly inclusive health policies for foreigners/migrants, throughout the country, were identified and discussed from an 'Health Equity' perspective. This enabled a small editorial group to draw up the basic document that, in the last session of the Course, was discussed and modified by all participants into the version of the 'Erice 58 Charter' presented here.


Assuntos
Saúde Pública , Migrantes , Humanos , Adulto , Saúde Pública/educação , Higiene , Itália , Sicília , Instituições Acadêmicas
2.
Environ Res ; 194: 110517, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33271142

RESUMO

The Erice 56 Charter titled "Impact of the environment on the health: from theory to practice" was unanimously approved at the end of the 56th course of the "International School of Epidemiology and Preventive Medicine G. D'Alessandro" held from 3rd to November 7, 2019 in Erice - Sicily (Italy) and promoted by the Study Group of "Environment and Health" of the Italian Society of Hygiene, Preventive Medicine and Public Health. The course, that included lectures, open discussions and guided working groups, was aimed to provide a general training on epidemiological and toxicological aspects of the environmental health impact, to be used by public health professionals for risk assessment, without forgetting the risk communications. At the end of the course 12 key points were agreed among teachers and students: they underlined the need of specific training and research, in the perspective of "One Health" and "Global Health", also facing emerging scientific and methodological issues and focusing on communication towards stakeholders. This Discussion highlight the need to improve knowledge of Health and Environment topic in all sectors of health and environmental prevention and management.


Assuntos
Saúde Ambiental , Saúde Pública , Saúde Global , Humanos , Sicília
3.
Ann Ig ; 31(6): 523-532, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31637905

RESUMO

The Erice 2018 Charter was unanimously approved at the conclusion of the 53rd Residential Course of the International School of Epidemiology and Preventive Medicine "Adapted Physical Activity in Sport, Wellness and Fitness; the role of the Departments of Prevention and of the National Health Service in doping prevention and health promotion", held on 15-19 May 2018 in Erice, Italy, at the "Ettore Majorana" Foundation and Centre for Scientific Culture, and promoted by the Study Group on "Movement Sciences for Health" of the Italian Society of Hygiene, Preventive Medicine and Public Health. The event was part of a larger project supported by the Ministry of Health aimed at preventing doping in the general population involved in sport and physical activities. After an intense discussion the participants focused on ten statements involving the following critical issues: responsibility, priority, message, alphabetization, networks and alliances, school promoting health, player and opportunities, competences, know-how, programming and acting. These statements provide hints to approach doping within a public health frame and summarize the role of the Departments of Prevention and NHS in promoting and coordinating preventive actions with other institutions and stakeholders. Doping represents a complex phenomenon related to cultural, social, economic and legal issues. In addition to regulatory or repressive actions, education to health and legality is proposed as the fundamental strategy to contrast doping by promoting healthy lifestyles, based on scientific knowledge and respect for legality.


Assuntos
Dopagem Esportivo/prevenção & controle , Promoção da Saúde/métodos , Saúde Pública , Humanos , Itália , Medicina Estatal/organização & administração
4.
J Virol Methods ; 268: 48-52, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30902644

RESUMO

Rotavirus, adenovirus, norovirus and astrovirus are considered to be among the major causes of sporadic cases and outbreaks of acute gastroenteritis globally. Rapid and accurate identification of enteric viruses is still a challenge for the clinical laboratory. Recently, several molecular platforms for the detection of viral enteric pathogens have become available. In this study, the diagnostic accuracy of InGenius Gastrointestinal Viral (GV) Elite Panel, a newly developed one-step multiplex real-time RT-PCR assay simultaneously detecting rotavirus, adenovirus and astrovirus, was evaluated retrospectively analyzing an archival collection of 128 stool samples of children hospitalized with acute gastroenteritis. The overall sensitivity and specificity for the GV assay was 100% and 96.2% for rotavirus, 96.9% and 100% for astrovirus, 100% and 100% for adenovirus, respectively. The InGenius GV assay showed a high concordance with the reference methods and was able to detect all tested genotypes of rotavirus (including G1, G3, G4, G9 and G12P[8] and G2P[4]), adenovirus and astrovirus (AstV-1 and 2). Studies of considerable sample size are required to determine robust Cycle threshold cut-off values to effectively correlate infection to disease. These preliminary results suggest that InGenius GV assay can be recommended as a valuable method for accurate diagnosis of epidemic and sporadic gastroenteritis.


Assuntos
Infecções por Adenoviridae/diagnóstico , Infecções por Astroviridae/diagnóstico , Gastroenterite/diagnóstico , Reação em Cadeia da Polimerase Multiplex , Infecções por Rotavirus/diagnóstico , Doença Aguda , Adolescente , Criança , Pré-Escolar , Fezes/virologia , Feminino , Gastroenterite/virologia , Humanos , Lactente , Recém-Nascido , Masculino , Estudos Retrospectivos , Sensibilidade e Especificidade
5.
Epidemiol Infect ; 146(7): 879-887, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29633676

RESUMO

In May 2016 a Norovirus (NoV) gastroenteritis outbreak involved a high school class visiting a seaside resort near Taormina (Mascali, Sicily). Twenty-four students and a teacher were affected and 17 of them showed symptoms on the second day of the journey, while the others got ill within the following 2 days. Symptoms included vomiting, diarrhoea and fever, and 12 students required hospitalisation. Stool samples tested positive for NoV genome by Real-Time polymerase chain reaction assay in all 25 symptomatic subjects. The GII.P2/GII.2 NoV genotype was linked to the outbreak by ORF1/ORF2 sequence analysis. The epidemiological features of the outbreak were consistent with food/waterborne followed by person-to-person and/or vomit transmission. Food consumed at a shared lunch on the first day of the trip was associated to illness and drinking un-bottled tap water was also considered as a risk factor. The analysis of water samples revealed the presence of bacterial indicators of faecal contamination in the water used in the resort as well as in other areas of the municipal water network, linking the NoV gastroenteritis outbreak to tap water pollution from sewage leakage. From a single water sample, an amplicon whose sequence corresponded to the capsid genotype recovered from patients could be obtained.


Assuntos
Infecções por Caliciviridae/epidemiologia , Surtos de Doenças , Água Potável/virologia , Gastroenterite/epidemiologia , Norovirus/fisiologia , Doenças Transmitidas pela Água/epidemiologia , Adolescente , Infecções por Caliciviridae/virologia , Feminino , Gastroenterite/virologia , Humanos , Masculino , Sicília/epidemiologia , Doenças Transmitidas pela Água/virologia
6.
Ann Ig ; 29(6): 481-493, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29048447

RESUMO

The Erice 50 Charter titled "Strategies for Diseases Prevention and Health Promotion in Urban Areas" was unanimously approved at the conclusion of the 50th Residential Course "Urban Health. Instruments for promoting health and for assessing hygienic and sanitary conditions in urban areas", held from 29th March to 2nd April 2017 in Erice, at the "Ettore Majorana" Foundation and Centre for Scientific Culture and promoted by the International School of Epidemiology and Preventive Medicine "G. D'Alessandro" and the Study Group "Building Hygiene" of the Italian Society of Hygiene, Preventive Medicine and Public Health (SItI). At the conclusion of the intense learning experience during the Course, with more than 20 lectures, workshops and long-lasting discussions between Professors and Students, the participants identified the major points connecting urban features and Public Health, claiming the pivotal role of urban planning strategies for the management of Diseases Prevention and Health Promotion activities. The Erice 50 Charter is configured as a Decalogue for Healthy Cities and as a Think Tank for designing effective strategic actions and best practices to develop urban regeneration interventions and improve the urban quality of contemporary cities. The Decalogue is structured into the following key strategic objectives: 1. Promoting urban planning interventions that address citizens towards healthy behaviours; 2. Improving living conditions in the urban context; 3. Building an accessible and inclusive city, with a special focus on the frail population; 4. Encouraging the foundation of resilient urban areas; 5. Supporting the development of new economies and employment through urban renewal interventions; 6. Tackling social inequalities; 7. Improving stakeholders' awareness of the factors affecting Public Health in the cities; 8. Ensuring a participated urban governance; 9. Introducing qualitative and quantitative performance tools, capable of measuring the city's attitude to promote healthy lifestyles and to monitor the population's health status; 10. Encouraging sharing of knowledge and accessibility to informations. Finally, all the participants underlined that a multidisciplinary team, composed of Physicians specialized in Hygiene, Preventive Medicine, Public Health and Technicians as Architects, Urban planners and Engineers, is needed to deepen the research topic of Urban Health.


Assuntos
Promoção da Saúde/métodos , Prevenção Primária/métodos , Saúde da População Urbana , Humanos , Itália
7.
Ann Agric Environ Med ; 24(2): 303-306, 2017 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-28664713

RESUMO

Introduction and objective. The introduction of a vaccine against hepatitis B virus (HBV) for newborn babies in Italy in 1991, extended to 12-year-old children for the first 12 years of application, has been a major achievement in terms of the prevention of HBV infection. The objective of this study was to analyse the long-term immunogenicity and effectiveness of HBV vaccination among healthcare students with different working seniorities. Materials and method. A cross-sectional observational study of undergraduate and postgraduate students attending the Medical School of the University of Palermo was conducted from January 2014 - July 2016. HBV serum markers were performed with commercial chemiluminescence assays. Categorical variables were analyzed using the chi-square test (Mantel-Haenszel), whereas means were compared by using the Student's t test. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were also calculated by a multivariable logistic regression, using a model constructed to examine predictors of anti-HBs titer above 10 mIU/mL, assumed as protective. Results. Of the 2,114 subjects evaluated - all vaccinated at infancy or at the age of 12 years and were HBsAg/anti-HBc negative - 806 (38.1%) had an anti-HBs titre <10 IU/L. The latter were younger, more likely to be attending a healthcare profession school (i.e., nursing and midwifery), than a medical postgraduate level school, and more likely to have been vaccinated in infancy (p <0.001, 95% CI 2.63-5.26, adjusted OR 3.70). Conclusion. The results of the study suggest that assessment of HBV serum markers in workers potentially exposed to hospital infections is useful for identifying small numbers of unvaccinated subjects, or vaccinated subjects with low antibody titre, all of whom should be referred to a booster series of vaccinations.


Assuntos
Antígenos de Superfície da Hepatite B/imunologia , Vacinas contra Hepatite B/imunologia , Vírus da Hepatite B/imunologia , Hepatite B/prevenção & controle , Adolescente , Adulto , Biomarcadores/sangue , Criança , Estudos de Coortes , Estudos Transversais , Feminino , Seguimentos , Hepatite B/imunologia , Hepatite B/virologia , Anticorpos Anti-Hepatite B , Antígenos de Superfície da Hepatite B/administração & dosagem , Vacinas contra Hepatite B/administração & dosagem , Vírus da Hepatite B/genética , Humanos , Masculino , Faculdades de Medicina , Estudantes , Estudantes de Medicina , Adulto Jovem
8.
J Virol Methods ; 243: 50-54, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-28159668

RESUMO

Group A rotaviruses (RVAs) are the primary cause of acute gastroenteritis (AGE) in young children worldwide. Several commercial tests including latex agglutination, enzyme-linked assays (ELISA) and immunochromatographic tests (ICT) have been developed for the diagnosis of RVA infection. In the present study, the performance of two commercially available one-step chromatographic immunoassays, CerTest Rotavirus+Adenovirus (Biotec S.L, Zaragoza, Spain) and Vikia Rota-Adeno (bioMerieux SA, Lyon, France) were retrospectively evaluated using Real-time PCR as reference test. Re-testing by Real-time PCR of 2096 stool samples of children hospitalized with AGE previously screened by ICTs (1467 by CerTest and 629 by Vikia) allowed to calculate higher sensitivity for Vikia (94% vs 85% of CerTest) and higher specificity for CerTest (93% vs 89% of Vikia). Accordingly, higher Positive Predictive Values (87% vs 78%) and Positive Likelihood Ratios (12.32 vs 8.8) were found for CerTest and lower Negative Predictive Values (91% vs 97%) and Negative Likelihood Ratios (0.16 vs 0.06) for Vikia. However, both CerTest and Vikia showed a substantial agreement (κ=0.79) with the Real-time PCR. A correlation between false negative results by ICTs and high Cycle Threshold values of Real-time PCR, indicative of low viral load, was observed. False positive results by the two ICT assays were not related to Norovirus, Adenovirus or Astrovirus infections, therefore the risk of cross-reactions was excluded. Both CerTest and VIKIA were able to detect the wide range of RVA genotypes circulating over the study period (including G1P[8], G2P[4], G3, G4, G9 and G12P[8]). The results of the present study showed a satisfactory efficacy of the two diagnostic tests analyzed.


Assuntos
Cromatografia de Afinidade/métodos , Infecções por Rotavirus/diagnóstico , Pré-Escolar , Erros de Diagnóstico , Feminino , Gastroenterite/diagnóstico , Humanos , Lactente , Recém-Nascido , Itália , Masculino , Valor Preditivo dos Testes , Estudos Retrospectivos , Sensibilidade e Especificidade
9.
Epidemiol Infect ; 144(9): 1943-50, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-26743189

RESUMO

Genotype G12 strains are now considered to be the sixth most prevalent human rotaviruses worldwide. In two Sicilian cities, Palermo and Messina, surveillance of rotavirus circulation performed since 1985 and 2009, respectively, did not detect G12 strains until 2012. From 2012 to 2014 rotavirus infection was detected in 29·7% of 1647 stool samples collected from children admitted for acute gastroenteritis to three Sicilian hospitals in Palermo, Messina and Ragusa. In 2012, G12P[8] was first detected in Palermo and then in Messina where it represented the second most frequent genotype (20% prevalence) after G1P[8]. Thereafter, G12 strains continued to circulate in Sicily, showing a marked prevalence in Ragusa (27·8%) in 2013 and in Palermo (21%) and Messina (16·6%) in 2014. All but one of the Sicilian G12 strains carried a P[8] VP4 genotype, whereas the single non-P[8] rotavirus strain was genotyped as G12P[9]. Phylogenetic analysis of the VP7 and VP4 sequences allowed distinction of several genetic lineages and separation of the G12P[8] strains into three cluster combinations. These findings indicate independent introductions of G12 rotavirus strains in Sicily in recent years.


Assuntos
Genótipo , Infecções por Rotavirus/epidemiologia , Infecções por Rotavirus/virologia , Rotavirus/classificação , Rotavirus/isolamento & purificação , Adolescente , Antígenos Virais/genética , Proteínas do Capsídeo/genética , Criança , Pré-Escolar , Cidades , Análise por Conglomerados , Fezes/virologia , Feminino , Gastroenterite/epidemiologia , Gastroenterite/virologia , Humanos , Lactente , Masculino , Filogenia , Prevalência , Rotavirus/genética , Análise de Sequência de DNA , Sicília/epidemiologia
10.
Virus Genes ; 50(2): 310-5, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25634124

RESUMO

A canine Rotavirus A strain was identified in the fecal specimen of a young dog during 2012 in Hungary. The strain RVA/Dog-wt/HUN/135/2012/G3P[3] shared complete genotype constellation (G3-P[3]-I3-R3-C3-M3-A15-N2-T3-E3-H6) and high genome sequence similarity (nt, 98.8 %) with a historic human strain, RVA/Human-tc/ITA/PA260-97/1997/G3P[3]. This study provides evidence for the canine origin of the unusual NSP1 genotype, A15, and reinforces the hypothesis of direct interspecies transmission of canine rotaviruses to humans.


Assuntos
Doenças do Cão/virologia , Genoma Viral , Infecções por Rotavirus/veterinária , Infecções por Rotavirus/virologia , Rotavirus/genética , Rotavirus/isolamento & purificação , Animais , Sequência de Bases , Cães , Humanos , Hungria , Itália , Dados de Sequência Molecular , Filogenia , Rotavirus/química , Rotavirus/classificação , Homologia de Sequência do Ácido Nucleico , Proteínas Virais/química , Proteínas Virais/genética
11.
Virology ; 450-451: 355-8, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24503099

RESUMO

Global surveillance for norovirus identified in 2012 the emergence of a novel pandemic GII.4 variant, termed Sydney 2012. In Italy, the novel pandemic variant was identified as early as November 2011 but became predominant only in the winter season 2012-2013. Upon sequencing and comparison with strains of global origin, the early Sydney 2012 strains were found to differ from those spreading in 2012-2013 in the capsid (ORF2) putative epitopes B, C and D, segregating into a distinct phylogenetic clade. At least three residues (333, 340 and 393, in epitopes B, C and D, respectively) of the VP1 varied among Sydney 2012 strains of different clades. These findings suggest that the spread of the pandemic variant in Italy during the winter season 2012-2013 was due to the introduction of strains distinct from those circulating at low frequency in the former winter season and that similar strains were also circulating elsewhere worldwide.


Assuntos
Proteínas do Capsídeo/genética , Gastroenterite/virologia , Mutação , Norovirus/genética , Sequência de Aminoácidos , Proteínas do Capsídeo/metabolismo , Gastroenterite/epidemiologia , Humanos , Itália/epidemiologia , Dados de Sequência Molecular , Norovirus/classificação , Norovirus/isolamento & purificação , Norovirus/fisiologia , Pandemias , Filogenia , Estações do Ano
12.
J Clin Microbiol ; 51(11): 3855-7, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23966499

RESUMO

During 2012, a novel pandemic GII.4 norovirus variant, Sydney 2012, emerged worldwide. A signature of the variant was a GII.Pe ORF1, in association with GII.4 Apeldoorn 2008-like ORF2-ORF3 genes. We report the detection of recombinant GII.4 Sydney 2012 strains, possessing the ORF1 gene of the former pandemic variant New Orleans 2009.


Assuntos
Infecções por Caliciviridae/virologia , Norovirus/classificação , Norovirus/genética , Recombinação Genética , Infecções por Caliciviridae/epidemiologia , Criança , Pré-Escolar , Humanos , Dados de Sequência Molecular , Norovirus/isolamento & purificação , Fases de Leitura Aberta , Pandemias , RNA Viral/genética , Análise de Sequência de DNA
13.
Ann Ig ; 25(3): 263-9, 2013.
Artigo em Italiano | MEDLINE | ID: mdl-23598809

RESUMO

"Mediterranean diet" is commonly defined as a type of diet based on traditional foods of the Southern Italian regions, assuming that in the past the southern populations enjoyed a balanced and healthy diet. In fact, up to the middle of the twentieth century, widespread poverty in large parts of the population led to malnutrition due to lack of calories and essential nutrients. Only among the upper classes consumption of food was reasonable and respectful of the recommendations of the "Mediterranean diet pyramid". The fact remains that many traditional dishes can be recommended because they are well balanced on nutrients, tasty and appetizing.


Assuntos
Dieta Mediterrânea/história , Ingestão de Energia , Comportamento Alimentar , Nível de Saúde , Desnutrição/história , Pobreza/história , Dieta/história , História do Século XIX , História do Século XX , História do Século XXI , Humanos , Itália , Fatores de Risco , Sicília
14.
Epidemiol Infect ; 141(3): 524-8, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22592003

RESUMO

The study investigated the genetic diversity of human astroviruses (HAstVs) detected in children hospitalized with gastroenteritis in Italy in 2008-2009. A total of 1321 faecal samples were collected in Parma (northern Italy), Bari (southern Italy), and Palermo (Sicily) and screened for the presence of HAstVs. RT-PCR amplification of a portion at the 5'-end of ORF2 allowed the detection of HAstVs in 3·95% of the patients. Four different genotypes (HAstV-1, HAstV-2, HAstV-4, HAstV-5) were found to be circulating during the study period, with HAstV-1 being the predominant type. Interestingly, a novel lineage, proposed as HAstV-2d, was found to have emerged in Parma in 2009. Investigating the genetic variability of HAstVs will be important for understanding the epidemiological trends and evolution of these viruses.


Assuntos
Infecções por Astroviridae/epidemiologia , Infecções por Astroviridae/virologia , Mamastrovirus/genética , Vigilância da População , Pré-Escolar , Fezes/virologia , Genótipo , Humanos , Lactente , Itália/epidemiologia , Mamastrovirus/isolamento & purificação , Prevalência
15.
J Prev Med Hyg ; 54(4): 223-6, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24779285

RESUMO

INTRODUCTION: The purpose of this study was to investigate the antibacterial effect of 71 locally produced honeys from different botanical sources collected from apiarist's open markets in Sicily. METHODS: Antimicrobial activity was determined against Escherichia coli (ATCC 25922) and Salmonella serovar Infantis (ATCC 1523) by an agar-diffusion assay from the estimation of the diameter of the inhibition zone produced by the honeys. Statistically) significant differences (P < .000) regarding inhibition were observed for the honeys tested. RESULTS: The chestnut and polyfloral honey samples exhibited the largest and highest inhibition (diameter of the inhibition zone > 25 mm) against both E. coli and S. Infantis. The honey of oregano origin showed intermediate or low activity against E. coli and S. Infantis, respectively. Prickly pear and erica honeys showed no antimicrobial activity against the two reference strains. DISCUSSION: The results may partially suggest the usefulness of the Sicilian honeys on treating multi-resistant enterobacteria. In light of the enormous potential for application of honey in the clinical practice, it is important that research continues not only into those honeys well recognized as antimicrobial, but also into other locally produced and yet untested honeys.


Assuntos
Antibacterianos/farmacologia , Escherichia coli/efeitos dos fármacos , Mel , Salmonella/efeitos dos fármacos , Itália , Testes de Sensibilidade Microbiana
16.
Iran Red Crescent Med J ; 14(7): 408-16, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22997556

RESUMO

BACKGROUND: Urinary tract infection is the most common health problem affecting millions of people each year, mainly caused by a large genetically heterogeneous group of Escherichia coli called uropathogenic E. coli This study investigates the genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. METHODS: During 2008-2009, 90 E. coli strains were analyzed, consisting of 48 isolates causing pyelonephritis in children and 42 isolates causing cystitis. Having identified the strains by standard methods, they were subtyped by pulsed field gel electrophoresis (PFGE) and their corresponding patterns were compared using dendrogram. RESULTS: Sixty five PFGE profiles were obtained from the genome of E. coli strains by this genotyping method. Thirty six and thirty three patterns were obtained for pyelonephritis and cystitis, respectively. Most strains exhi-bited twelve and thirteen bands and the patterns with eight or nineteen bands had the lowest rate. Genome sizes of the strains were between 1610-4170 kbp. CONCLUSION: With due attention to these results, genetic patterns showed that the strains had different clonalities and it could be suggested in some cases that the strains causing pyelonephritis or cystitis have common patterns and different diseases could be explained by different gene factors.

17.
J Clin Microbiol ; 50(11): 3760-4, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22933603

RESUMO

Novel lineages of human astrovirus (HAstV) types 2, 2c, and 2d have been identified. Upon sequencing of the 3' end of the genome, the type 2c and 2d HAstVs were found to be open reading frame 1b (ORF1b)-ORF2 recombinant, with ORF1b being derived from type 3 and type 1 HAstVs, respectively. An ORF2 interlineage recombinant strain, 2c/2b, was also identified.


Assuntos
Heterogeneidade Genética , Mamastrovirus/classificação , Mamastrovirus/genética , Recombinação Genética , Análise por Conglomerados , Humanos , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , RNA Viral/genética , Análise de Sequência de DNA
18.
Med Lav ; 103(2): 141-5, 2012.
Artigo em Italiano | MEDLINE | ID: mdl-22619990

RESUMO

BACKGROUND: Italian Legislative Decree No. 81/2008 foresees the involvement of a "specialised physician" in activities for the promotion of health at the workplace. OBJECTIVES: These activities are aimed at modifying erroneous lifestyles (such as smoking, drinking, bad diet, etc.) which are responsible for serious illnesses and also for repercussions on the assessment of fitness for a specific task. Obesity, as we know, represents the major risk factor in the onset of metabolic, neoplastic, cardiovascular and respiratory diseases and also in the increased incidence of accidents at the workplace and sick absence. METHODS: A specific questionnaire was administered to 700 health care workers of a Hospital in Catania to estimate the incidence of factors which favour the onset of these diseases: factors such as familiarity, endocrinal dysfunction problems (diabetes mellitus, hypothyroidism), shift work and little physical exercise. RESULTS AND CONCLUSION: Statistical processing of the data confirmed, partially, that little physical exercise, hypothyroidism, diabetes mellitus, familiarity in the > 36 age group were the major risk factors in the onset of obesity, as reported in the literature. However, contrary to the literature reports, shift work did not seem to be a risk factor in the sample under study. A programme of rehabilitation, on a voluntary basis, will be offered to those workers who are overweight/obese. This programme will involve professionals such as nutritionists, endocrinologists and psychologists, and it will aim at improving workers' health conditions as well as their work performance.


Assuntos
Pessoal de Saúde/estatística & dados numéricos , Promoção da Saúde , Obesidade/epidemiologia , Obesidade/prevenção & controle , Consumo de Bebidas Alcoólicas/efeitos adversos , Índice de Massa Corporal , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/prevenção & controle , Complicações do Diabetes/epidemiologia , Complicações do Diabetes/prevenção & controle , Diabetes Mellitus/epidemiologia , Diabetes Mellitus/prevenção & controle , Hospitais Gerais , Humanos , Incidência , Estilo de Vida , Obesidade/complicações , Obesidade/diagnóstico , Fatores de Risco , Sicília/epidemiologia , Fumar/efeitos adversos , Inquéritos e Questionários , Local de Trabalho
19.
J Gen Virol ; 92(Pt 5): 1214-1221, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21228122

RESUMO

The full-length genome sequence of a feline G3P[9] rotavirus (RV) strain, BA222, identified from the intestinal content of an adult cat, was determined. Strain BA222 possessed a G3-P[9]-I2-R2-C2-M2-A3-N1-T3-E2-H3 genomic constellation, differing substantially from other feline RVs. Phylogenetic analyses of each genome segment revealed common origins with selected animal and zoonotic human RVs, notably with rare multi-reassortant human G3P[9] RVs (Ita/PAI58/96 and Ita/PAH136/96). Altogether, the findings suggest that feline RVs are genetically diverse and that human RVs may occasionally originate either directly or indirectly (via reassortment) from feline RVs.


Assuntos
Vírus Reordenados/genética , Vírus Reordenados/isolamento & purificação , Rotavirus/genética , Rotavirus/isolamento & purificação , Animais , Gatos , Análise por Conglomerados , Humanos , Dados de Sequência Molecular , Filogenia , RNA Viral/genética , Análise de Sequência de DNA , Homologia de Sequência
20.
Clin Microbiol Infect ; 17(1): 97-101, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20219080

RESUMO

By screening faecal samples collected over four consecutive years (2002-2005) from hospitalized children with diarrhoea in Palermo, Italy, astroviruses (HAstVs) were detected in 3.95% of the patients. The predominant type circulating was HAstV-1 but, in 2002, only HAstV-2 and -4 were identified. Interestingly, the HAstVs-2 detected appeared to be consistently different in 5' end of their open reading frame 2 from the previously described subtypes. These novel type 2 strains were included in a new 2c lineage based on the phylogenetic analysis and the presence of nine peculiar substitutions.


Assuntos
Infecções por Astroviridae/epidemiologia , Infecções por Astroviridae/virologia , Mamastrovirus/genética , Criança , Fezes/virologia , Humanos , Itália/epidemiologia , Mamastrovirus/classificação , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , Polimorfismo de Nucleotídeo Único/genética , Vigilância da População , Prevalência , RNA Viral/genética
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