RESUMO
The aim of this study was to assess alterations of zeta chain expression and their relation to apoptosis of T lymphocytes. T lymphocytes were obtained from malignant pleural effusions (MPE) of 15 patients. The work focused on TCR-zeta chain expression, apoptosis of T lymphocytes, and on the content of monocyte/macrophages in effusions. Analysis was performed using three color flow cytometry combining CD3, TCR-zeta and TUNEL reaction. The content of tumor and monocyte/macrophage cells has been determined using CD3, CD14, and cytokeratins, as markers of distinct cell subpopulations. Our findings strongly indicate that decreased zeta chain expression in T cells depends on the content of monocyte/macrophage cells, and that the range of the decrease is inversely proportional to the number of monocytes/macrophages in the effusion. Those having low zeta chain expression were the main subpopulation of T cells undergoing apoptosis. These data suggest that decreased zeta chain expression in T cells in MPE may be related to the abundance of monocyte/macrophages in effusions.
Assuntos
Apoptose , Regulação para Baixo , Macrófagos/metabolismo , Proteínas de Membrana/biossíntese , Monócitos/metabolismo , Receptores de Antígenos de Linfócitos T/biossíntese , Linfócitos T/metabolismo , Complexo CD3/biossíntese , Citometria de Fluxo , Humanos , Marcação In Situ das Extremidades Cortadas , Receptores de Lipopolissacarídeos/biossíntese , Neoplasias/metabolismo , Derrame Pleural/metabolismo , Derrame Pleural Maligno/metabolismoRESUMO
Staphylococcus aureus is a frequent pathogen of nosocomial infections. The main part in the spread of these microorganisms take symptomless carriers. The aim the research was defining the carrierstate of S. aureus among students of Medical Academy and University. The investigation showed a greater carrierstate in the group of Medical students (33%) than in the group of University students. Strains isolated from the Medical students were more differentiated in biochemical tests and they were more drug-resistant mainly to Augmentin (51.5% resistant strains) and doxycycline (24% resistant strains). A great percentage of ampicillin-resistant strains (94%) was found among the strains isolated from both groups. Results of the research showed greater carrierstate among people who had direct contact with patients and infectious materials and proved a wide range of drug-resistance among hospital strains. Carriers of S. aureus among medical personnel could influence the spreading of nosocomial infections mainly on ICU and Newborn Wards.
