The effect of weaner diet protein content and diet quality on the long-term performance of pigs to slaughter.

Short and long-term effects of manipulating dietary CP content and diet quality in weaner diets on health and performance of pigs were investigated in a 2 x 2 factorial combination of CP inclusion (high-CP, 230 g of CP/kg vs. low-CP, 170 g of CP/kg) and diet quality (high-quality, cooked cereals, and animal protein vs. low-quality, raw cereals, and plant protein). Diets were fed ad libitum for 14 d postweaning to pigs weaned at 29.4+/-3.1 d of age and 9.9+/-1.0 kg of BW. From d 14 to slaughter at 104+/-3 kg, all pigs were fed the same series of standard commercial diets. There were 15 replicates per treatment in the weaner phase (<30 kg) and 5 replicates per treatment in the grower-finisher phase (>30 kg). High-quality diets promoted gut health as indicated by improved fecal lactobacilli to coliform ratio (P=0.002) and decreased fecal enterotoxigenic Escherichia coli counts on d 11 postweaning (P=0.028), reducing the risk of postweaning diarrhea and improving pig health from weaning to the end of the weaner phase. Reducing CP content had no effect on gut health. High-CP (P=0.053) and high-quality (P=0.025) diets independently increased ADG during the first 14 d postweaning compared with low-CP and low-quality diets, respectively. There were no interactions between dietary CP content and quality on any of the response criteria investigated. Despite differences in the immediate postweaning period, there was no effect of manipulating diet quality or CP content for 2 wk postweaning on lifetime performance with pigs reaching slaughter weight in 128+/-7 d. These results indicate that high-quality diets may protect pig gut health during the immediate postweaning period. However, it may be possible to use less expensive, decreased quality weaner diets without any adverse effects on long-term performance when weaning older, heavier pigs and where health status, environmental control, and stock management are all maintained to a high standard.

Body composition of breeding gilts in response to dietary protein and energy balance from thirty kilograms of body weight to completion of first parity.

The relationships among BW, backfat depth, and body physical and chemical composition were evaluated in response to dietary protein and DE balance in breeding gilts from 30 kg of BW to weaning of the first litter. Large White (sire) x Landrace (dam) F1 hybrid (White; n = 75) and Landrace (sire) x (Meishan x Large White; dam) (Meishan; n = 19) hybrid gilts were received at 30 kg of BW. Five gilts were taken as the initial slaughter group at 30 kg of BW, and the remaining gilts were fed diets differing in total lysine to DE ratio, high (H) vs. low (L), from 30 kg of BW to mating (rearing), and during gestation and lactation, allowing factorial investigation of dietary treatment effects and interactions during rearing, gestation, and lactation. Gilts were slaughtered at approximately 50 and 90 kg of BW, and at mating, farrowing, and weaning. Gilts fed L diets during rearing were lighter at mating (117.9 vs. 133.6 kg of BW, P = 0.035) due to a reduction in gain (592 vs. 720 g/d, P = 0.002) and a restriction in protein accretion (83 vs. 117 g/d, P = 0.001). During rearing, lipid accretion did not differ between L- and H-fed gilts (208 vs. 198 g/d, P = 0.60), but the ratio of lipid to protein accretion was about 1.5-fold greater in L-fed gilts, where lipid mass expressed as a percentage of BW was increased at mating (26.0 vs. 21.9%, P = 0.005). Effects of L diets on lipid accretion during rearing were transient; no residual effects on body lipid mass (P > 0.17) were found at farrowing or weaning. Overall, Meishan hybrids carried greater lipid mass (P < 0.001) than White hybrid gilts. Whereas the rate of body lipid and protein accretion and body lipid and protein mass can be nutritionally influenced and can vary according to growth stage, reproductive status, and genotype, this study established that body protein mass expressed as a proportion of the lipid free empty BW remains inflexible. A value for this measure of 0.188 +/- 0.0052 was found in White and Meishan hybrid gilts ranging from 28 to 203 kg of BW and 3 to 36 mm backfat depth, covering growth, pregnancy, and lactation, and offered diets differing in protein and energy balance. Body protein mass can be predicted as approximately 0.2 of the lipid free empty BW once body lipid mass is estimated accurately from physical measurements, such as backfat depth (P2, mm) and BW (kg), by regression using lipid (kg) = - 8.14 (SE, 1.302) + 0.167 (SE, 0.010) BW + 0.883 (SE, 0.065) P2 (residual SD = 3.51; R2 = 0.912).

Feeding linseed to increase the n-3 PUFA of pork: fatty acid composition of muscle, adipose tissue, liver and sausages.

Eighty pigs, male and female littermate pairs, were fed a control or a test diet from 25 to 95 kg live weight. The diets, as fed, contained 15.5 g/kg linoleic acid (18:2) and 1.9 g/kg α-linolenic acid (18:3) (control) or 10 g/kg linoleic acid and 4 g/kg α-linolenic acid (test). The test diet, with added linseed, was, therefore, high in the main n-3 polyunsaturated fatty acid (PUFA) 18:3 and low in the main n-6 PUFA 18:2. Making this relatively small change led to a 56% increase in the content of 18:3 in muscle and major increases in the contents of the beneficial longer chain PUFAs EPA (20:5n-3) (100% increase) and DHA (22:6n-3) (35% increase) which are synthesised from 18:3n-3. Levels of EPA and DHA in pigmeat adipose tissue were also increased by the test diet. In liver, the test diet resulted in an 18:3 level 4× higher than in muscle, with 10× more EPA and 20× more DHA. Sausages, analysed after 6 months frozen storage also had high n-3 PUFA levels, due to the contribution of these fatty acids from both muscle and adipose tissue. From a health perspective these results confirm the potential of pigmeat to supply valuable n-3 PUFA to the human diet. The test diet produced a PUFA:saturated FA ratio in muscle of 0.4, close to the minimum recommended value for the diet as a whole and an n-6:n-3 ratio of 5, a significant improvement on the current average for pigmeat (7). It is estimated that the test diet would provide 12 g of long chain n-3 PUFA to the human diet per annum at current pigmeat consumption levels in the UK, about a third of that from oily fish.

Shelf life and quality of pork and pork products with raised n-3 PUFA.

Pork chops, liver, bacon and sausages from 80 pigs fed on a control or a linseed-rich test diet were assessed for their sensory qualities and oxidative stability after conditioning and storage, either frozen or under simulated retail display. There was no significant effect of diet on lipid oxidation as assessed by the TBA test (P>0.05) for pork, liver and sausages, values being less than 0.2 throughout the display period, lower than the threshold of 0.5 mg MDA/kg at which consumers may detect rancidity. Nor was there any significant effect of diet on colour changes of pork chops, sausages and bacon under simulated retail display conditions. Grilled loin chops, bacon and sausages from male pigs received significantly higher abnormal flavour scores than those from females but panellists were unable to discern any major differences between control and test diets, even though they could detect this relatively small sex effect. The test diet resulted in higher α-linolenic acid (18:3) levels, with major increases in total n-3 PUFA content, but the concentrations were generally lower than reported values that have resulted in stability problems. The n-6 PUFA content was also reduced by the test diet. The results demonstrate that the nutritional value of pigmeat can be improved by using an 18:3-enriched diet without adversely affecting normal eating quality parameters.

Reciprocy and helicy used to relate mEGF and wound healing.

The Rogers homeodynamic principles and holistic conceptual framework from which they are derived are useful for describing, explaining, and predicting events that have a direct relevance for the professional practice of nursing. Reciprocy and helicy, principles that deal with the mutual interaction between human and environmental fields, and the unidirectionality of their curvilinear progression along the space--time dimension were useful in constructing a theoretical system for explaining the relationship of epidermal growth factor (EGF) and epidermal wound healing. Attachment of the polypeptide EGF to its receptor is viewed as the initial interaction of the human and environmental fields that eventually results in a change in the EGF and the cellular functions of the keratinocytes. At the empirical level, the EGF-keratinocyte interaction is indexed by changes in the keratinocyte migration rate, the mitotic index, and differentiation of cells over the wound. A pilot project to test the concept, using a young Yorkshire-mix pig, is described.

Serum phosphorus during the menstrual cycle.

In the continuing search for a simple, noninvasive, reliable method to detect ovulation, phosphorus (P) levels have been measured in women across a menstrual cycle. Salivary P has been reported to increase significantly in women periovulatory. Reports of serum P show no such periovulatory peak. We measured serum P in nine ovulatory and two anovulatory women during one menstrual cycle. Indirect indicators of ovulation were biphasic BBT curve and luteal phase serum progesterone more than 3 ng/ml. No significant periovulatory change in serum P was found, only small fluctations ranging from 0.99--2.76 mg/dl. The absence of a periovulatory serum phosphorus peak in this and other investigations of serum P suggests that if there is altered P metabolism during the menstrual cycle, it is not reflected in the serum. Other mechanisms, such as urinary or salivary excretion, may be operating to maintain serum P within the physiological range. This hypothesis needs to be explored.

PIP: Phosphorus levels were measured in sera throughout a menstrual cycle to determine if such measurements would provide a noninvasive means of detecting ovulation. Previous reports have established a periovulatory peak for salivary phosphorus, but no such elevation has been shown before for serum phosphorus, so this study examined changes in the serum level. 11 women, 9 ovulatory and 2 anovulatory were studied for 1 menstrual cycle. Serum samples for phosphorus determination were taken every other day; levels of serum phosphorus were compared with more conventional indicators of ovulation, such as basal body temperature increase at luteal phase and increased serum progesterone from 3 ng/ml or more. Small fluctuations were found (range, .99-2.76 mg/dl) in serum phosphorus but no periovulatory peak was determined. Since other studies have demonstrated a peak in salivary phosphorus, it is hypothesized that phosphorus changes do occur coupled with ovulation, but that these changes are not reflected in serum. The hypothesis of phosphorus metabolism change in relation to ovulation needs further research.