RESUMO
Since 1990, Laboratory Services, First Nations and Inuit Health Branch (Health Canada) conducted an interlaboratory comparison program for mercury in human hair. Laboratory Services initiated this program to compare the performance of participating laboratories, analyzing mercury in human hair samples by a variety of analytical methods and instrumental detection techniques. The results of the quality assurance program, which included 31 participants on four continents, are described. Of the participating laboratories, 92% consistently meet QA/QC performance limits for the determination of Hg in human hair. A variety of analytical methods using different digestion and instrumental techniques gave similar results. The most frequently used instrumental techniques were: CV-AA, CV-AFS, and ICP-MS. A summary of results from 24 rounds is provided. The feedback from this program has assisted some laboratories in improving their results and solving some of their analytical problems.
Assuntos
Exposição Ambiental , Cabelo/química , Mercúrio/análise , Humanos , Cooperação Internacional , Espectrometria de Massas , Microscopia de Fluorescência , Variações Dependentes do Observador , Controle de Qualidade , Valores de Referência , Sensibilidade e Especificidade , Espectrofotometria AtômicaRESUMO
A method for the determination of environmentally relevant toxaphene congeners (eg chlorinated bornanes (CHBs)) in serum is described. Four chlorinated bornane congeners are predominant in serum.
Assuntos
Inseticidas/sangue , Resíduos de Praguicidas/sangue , Toxafeno/sangue , Canadá , Isótopos de Carbono , Fracionamento Químico , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Inseticidas/química , Inseticidas/isolamento & purificação , Marcação por Isótopo , Resíduos de Praguicidas/química , Resíduos de Praguicidas/isolamento & purificação , Padrões de Referência , Reprodutibilidade dos Testes , Toxafeno/química , Toxafeno/isolamento & purificaçãoRESUMO
A method for the determination of environmentally relevant PCB congeners and organochlorine pesticides in human blood/serum is described. The method is validated through recovery experiments with standards and reproducibility of quality control samples.
Assuntos
Inseticidas/sangue , Bifenilos Policlorados/sangue , Técnicas de Química Analítica/métodos , Humanos , IsomerismoRESUMO
A new congener specific method has been developed for extraction and quantitative determination of PCB contaminants of serum. After liquid-liquid extraction and chromatographic fractionation with Carbopack-C/florisil column, the analytes were identified and quantified utilizing gas chromatography/mass spectrometry (GC/MSD) using selected ion monitoring (SIM) technique. Recoveries of 35 non-planar PCB congeners (#18, 28, 31, 44, 49, 52, 60, 66, 74, 82, 87, 99, 101, 105, 110, 114, 118, 128, 138, 141, 146, 151, 153, 156, 157, 158, 167, 170, 171, 180, 183, 185, 187, 189 and 194) and 5 non-ortho (coplanar) congeners (#37, 77, 81, 126 and 169) from spiked serum were between 55-115%.
