[Effect of delayed hypersensitivity on the development of an intradermal focus of a staphylococcal infection]. / Vliianie giperchuvstvitel'nosti zamedlennogo tipa na razvitie vnutrikozhnogo ochaga stafilokokkovoi infektsii.

Delayed hypersensitivity (DH) was induced in rabbits by introducing killed St. aureus culture in incomplete Freund's adjuvant. 7 days later the animals were infected intradermally with alpha-toxigenic and leukocidin-active St. aureus strains. Under these conditions DH shown to decrease the resistance of the organism of the infection: the infective agent could be isolated from the inoculation site and the regional lymph nodes for a longer period (5-6 days) than in healthy and immunized animals. The active penetration of the infective agent into the kidneys was observed. A suggestion was made that the development of DH resulted in the appearance of the potential possibility of auto- and reinfection in the body: starting from days 7-8 after the inoculated staphylococcal strains were eliminated from the organism, staphylococci could be again isolated from the kidneys and the lymph nodes. No connection between the toxinogenicity of St. aureus strain and the character of the development of the intradermal infection focus was revealed in the sensitized animals.

[Histopathology and histochemistry of a focus of staphylococcal infection in a sensitized body (material concerning pathogenesis)]. / K gistopatologii i gistokhimii ochaga stafilokokkovi infektsii v sensibilizirovannom organizme (materialy k patogenezu)

Rabbits were sensitized with a killed St. aureus culture (alpha-toxigenic 0-15 strain); one week later the were infected intradermally with a virulent leukocidin-active St. aureus V8 or with the 0-15 strain. A hyperegic inflammation with the signs of reaction observed in hypersensitivity of delayed type was revealed at the site of infection in 24 hours. On the seond day this process was supervened by an intensive leukocytic infiltration characteristic of this infection, with the subsequent suppuration. Beginning from the earliest stages of the process and up to its decline the inflammatory exudate displayed hyaluronic and sialic acids; they could perform functions of the nonspecific immunity factors in the focus. It was revealed at the terminal stage of the process that the organism did not react by the inflammatory reaction to the presence of the microbes remaining near the focus. Consequently, the tolerance of the organism to the microbe was revealed; this tolerance was possibly conditioned by the presence of sialic acids in the focus. Leukocytes with the phagocytized microbes appeared in the veins nearest to the focus. Histochemical shifts in the focus can be the cause of the incompleteness of phagocytosis. The tropism of staphylococci of the V8 strain to the collagen fibers was revealed.