Clinical evaluation of electronic dental anesthesia for various procedures in pediatric dentistry.

Electronic dental anesthesia (EDA) is a newer technique for achieving regional anesthesia by the application of transcutaneous electrical nerve stimulation (TENS). The present study intended to be an initial investigation of the attitudes of dentists and patients towards a relatively newly introduced EDA system. The purpose of the study was to evaluate the effectiveness of EDA system, a unit of TENS, in the management of pain during treatment procedures such as minor extractions, restorations (tooth preparations), and pulp therapy, in children. Overall, forty children between the age groups of 5 to 12 years were selected for three different clinical procedures. The response of the patients as well as the opinion of both, the clinician and the patient regarding perception of pain and comfort on administration I effectiveness of electronic anesthesia were recorded and analyzed. Significantly favorable results to the use of EDA were obtained, which indicate that EDA, besides offering safety and psychological advantages, may also be a promising alternative to the conventional methods of local anesthesia.

The reduction potential of cytochrome b5 is modulated by its exposed heme edge.

When the reduction potential of cytochrome b5 is measured with the aid of several different surface-modified electrodes that function on the basis of electrostatic interactions with the protein, the resultant values have been consistently more positive (40-100 mV) than the reduction potentials measured with potentiometric methods. In this paper, we report that the heme edge containing the exposed heme propionate, a heme methyl, and a heme vinyl, and which constitutes part of the surface of cytochrome b5, modulates its reduction potential. The positive shifts observed in the voltammetric measurements appear to originate from the formation of a complex between cytochrome b5 and the modified electrode surface which (a) neutralizes the charge on the heme propionate located on the exposed heme edge and (b) lowers the dielectric of the exposed heme microenvironment by excluding water from the complex interface, factors which result in the destabilization of the positive charge on the ferric heme with respect to the neutral ferrous heme. The observed positive shift, which is induced by complexation at the electrode surface, may indicate that similar shifts in the reduction potential of cytochrome b5 occur when it forms a complex with physiological partners, prior to electron transfer. The effect of the value of the dielectric constant on the reduction potential of cytochrome b5 was corroborated by preparing the V45L/V61L double mutant whose reduction potential was measured to be 50 mV more negative than the value measured for the wild type protein. The negative shift in the reduction potential of the mutant protein was explained by the increased accessibility of water to the heme binding site, as observed in its X-ray crystal structure.

Diagnosing respiratory syncytial virus by nasal lavage.

Nasal lavage was compared with nasopharyngeal aspiration for diagnosis of respiratory syncytial virus infection. Nasal lavage and nasopharyngeal aspiration were performed on 50 occasions in 32 infants (median age 5.6 months) with acute viral wheezing. Compared with nasopharyngeal aspiration, nasal lavage had a positive predictive value of 95.6% and negative predictive value of 92.5%. These comparable results and lack of adverse effects make nasal lavage the preferred method.

Antibody against viruses in maternal and cord sera: non-specific inhibitors are found to higher titre on the maternal side of the circulation.

Pregnancies were identified in which maternal IgG antibodies against rubella virus were not detectable by single radial haemolysis. Twenty paired maternal/cord sera were then tested for haemagglutination-inhibiting (HI) activity against rubella virus without kaolin pretreatment of the sera. In the absence of specific antibody, the HI activity observed could thus be ascribed to the effect of non-specific inhibitors. The HI activity in maternal sera was significantly (P less than 0.001) higher than that in cord sera. The 20 pairs of sera were similarly tested against a bunyavirus, an alphavirus and a flavivirus, both with and without kaolin pretreatment. The results showed non-specific inhibitors were found to higher titre in maternal sera, with the difference being statistically significant (P less than 0.001) for each of the three viruses.