High-Performance Liquid Chromatographic and High-Performance Thin-Layer Chromatographic Method for the Quantitative Estimation of Dolutegravir Sodium in Bulk Drug and Pharmaceutical Dosage Form.

Simple, sensitive, precise, and specific high-performance liquid chromategraphic (HPLC) and high-performance thin-layer chromatographic (HPTLC) methods for the determination of dolutegravir sodium in bulk drug and pharmaceutical dosage form were developed and validated. In the HPLC method, analysis of the drug was carried out on the ODS C18 column (150 × 4.6 mm, 5 µm particle size) using a mixture of acetonitrile: water (pH 7.5) in the ratio of 80:20 v/v as the mobile phase at the flow rate 1 mL/min at 260 nm. This method was found to be linear in the concentration range of 5-35 µg/mL. The peak for dolutegravir sodium was observed at 3.0 ± 0.1 minutes. In the HPTLC method, analysis was performed on aluminum-backed plates pre-coated with silica gel G60 F254 using methanol: chloroform: formic acid in the proportion of 8:2:0.5 v/v/v as the mobile phase. This solvent system was found to give compact spots for dolutegravir sodium with the Rf value 0.77 ± 0.01. Densitometric analysis of dolutegravir sodium was carried out in the absorbance mode at 265 nm. Linear regression analysis showed good linearity with respect to peak area in the concentration range of 200-900 ng/spot. The methods were validated for precision, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and specificity. Statistical analysis showed that both of the methods are repeatable and specific for the estimation of the said drug. The methods can be used for routine quality control analysis of dolutegravir sodium.

Economical spectrophotometric method for estimation of zaltoprofen in pharmaceutical formulations.

AIM: A simple, rapid, precise, and economical spectrophotometric method has been developed for quantitative analysis of zaltoprofen (ZLT) in pharmaceutical formulations. MATERIALS AND METHODS: A mixture of methanol and water was used as a solvent. Initial stock solution of ZLT was prepared in methanol and subsequent dilution was done in water. The standard solution of ZLT in water showed two absorption maxima, one at 243.5 nm and another at 338.0 nm. RESULTS: The drug obeyed Beer-Lambert's law in the concentration range of 1-40 µg/mL with regression 0.9999 at 243.5 nm and 5-100 µg/mL with regression 0.9999 at 338.0 nm. The overall % recovery was found to be 99.53% and 99.77% at 243.5 nm and 338.0 nm, respectively, which reflect that the method is free from interference of the impurities and other additives used in tablet formulation. Relative standard deviations of absorbance from six measurements were always less than 2%. CONCLUSIONS: The results of analysis have been validated as per ICH guidelines. Both the wavelengths can be adopted in routine analysis of ZLT in tablet dosage form.

Effect of different phases of menstrual cycle on physical working capacity in Indian population.

Women in large number are engaged in skilled and unskilled sectors of job and there is increasing participation of women in sports, Considering the fact that cyclical endocrine profile in females can have bearing on cardiovascular and respiratory function, in turn on physical capacity, The study of effects of phases of menstrual cycle on physical working capacity was undertaken in 40 female students of Bangalore Medical College. The subjects were instructed to come to the lab during each of three different phases of menstrual cycle. Resting heart rate (HR), respiratory rate (RR), BP were recorded. Subjects were made to exercise on Bicycle Ergometer and their maximum aerobic capacity was assessed as PWC170 (Physical working capacity at the heart rate 170). On statistical analysis we found resting RR and resting HR high during luteal phase with 'P' values of P < 0.01, P < 0.01 respectively. We also found PWC170 decreased during luteal and menstrual phase with 'P' values of P < 0.05 and P < 0.01. From the present study it can be concluded that resting HR and RR are increased during the luteal phase. Also PWC170 is decreased during the luteal and menstrual phases, this can have an adverse effect on the physical work output of females during these two phases.

Validated HPTLC Method for Simultaneous Determination of Quinapril Hydrochloride and Hydrochlorothiazide in a Tablet Dosage Form.

Quinapril hydrochloride and hydrochlorothiazide were simultaneously determined by HPTLC in pharmaceutical formulations. The drugs were separated on silica gel 60 F(254) plates using suitable combination of solvents as mobile phase. The validation parameters, tested in accordance with the requirements of ICH guidelines, prove the suitability of methods.

Fulminant citrobacter meningitis with multiple periventricular abscesses in a three-month-old infant

Citrobacter, a Gram-negative enteric bacillus, is a rare cause of septicemia and meningitis, seldom reported beyond the neonatal period. It is characterized by a fulminant clinical course and a high incidence of complications, including brain abscesses. We studied a three-month-old infant with Citrobacter meningitis, who developed acute communicating hydrocephalus and multiple periventricular brain abscesses while on treatment. The patient died, despite intensive antibiotic treatment directed towards the causative organism, C. diversus.

Fulminant citrobacter meningitis with multiple periventricular abscesses in a three-month-old infant.

Citrobacter, a Gram-negative enteric bacillus, is a rare cause of septicemia and meningitis, seldom reported beyond the neonatal period. It is characterized by a fulminant clinical course and a high incidence of complications, including brain abscesses. We studied a three-month-old infant with Citrobacter meningitis, who developed acute communicating hydrocephalus and multiple periventricular brain abscesses while on treatment. The patient died, despite intensive antibiotic treatment directed towards the causative organism, C. diversus.

Tubal pregnancy following laparoscopic sterilisation.

PIP: Laparoscopic sterilization with silastic band application is a common method of female sterilization in India. In 3 cases tubal pregnancies were found between 3 months and 6 years after sterilization. The mechanism is probably similar to that in ectopic pregnancy following tubectomy, either by formation of a tuboperitoneal fistula or because of incomplete occlusion of the tubal lumen, allowing the escape of sperm into the peritoneal cavity. 3 cases are reported.^ieng

A study of menarcheal age in India.

A study of menarcheal age was carried out in southern India. A logit method of analysis was applied to status quo data on 1267 Tamil and Telugu speaking girls aged 9 to 18 years in 3 schools catering for different socio-economic groups. There appears to be no relationship of menarcheal age with dietary pattern classified simply as vegetarian and non-vegetarian. Differences in median age at menarche between schools correspond well with the socio-economic differences between them. The median age in the most advantaged school (12-86 years) is comparable with that in recent studies in southern and eastern Europe, and may perhaps be in advance of some recent north-west European samples.