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ACS Sens ; 4(7): 1950-1956, 2019 07 26.
Artigo em Inglês | MEDLINE | ID: mdl-31310098

RESUMO

MicroRNA (miRNA) is a class of short RNA that is emerging as an ideal biomarker, as its expression level has been found to correlate with different types of diseases including diabetes and cancer. The detection of miRNA is highly beneficial for early diagnostics and disease monitoring. However, miRNA sensing remains difficult because of its small size and low expression levels. Common techniques such as quantitative real-time polymerase chain reaction (qRT-PCR), in situ hybridization and Northern blotting have been developed to quantify miRNA in a given sample. Nevertheless, these methods face common challenges in point-of-care practice as they either require complicated sample handling and expensive equipment, or suffer from low sensitivity. Here we present a new tool based on dark-field microwells to overcome these challenges in miRNA sensing. This miniaturized device enables the readout of a gold nanoparticle assay without the need of a dark-field microscope. We demonstrate the feasibility of the dark-field microwells to detect miRNA in both buffer solution and cell lysate. The dark-field microwells allow affordable miRNA sensing at a high throughput which make them a promising tool for point-of-care diagnostics.


Assuntos
Ensaios de Triagem em Larga Escala/métodos , Nanopartículas Metálicas/química , MicroRNAs/análise , Microscopia/métodos , DNA/química , DNA/genética , Desenho de Equipamento , Ouro/química , Ensaios de Triagem em Larga Escala/instrumentação , Ácidos Nucleicos Imobilizados/química , Ácidos Nucleicos Imobilizados/genética , Limite de Detecção , MicroRNAs/genética , Microscopia/instrumentação , Hibridização de Ácido Nucleico
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