Demographics of Giardia infections in Bergen, Norway, subsequent to a waterborne outbreak.

Young women have previously been reported as the predominant group infected during a waterborne giardiasis outbreak, due to elevated water consumption. Here, the demographics of those subsequently infected are described, and young women again predominate. As secondary cases were not waterborne, this cannot be attributed to drinking habits.

A longitudinal study on the occurrence of Cryptosporidium and Giardia in dogs during their first year of life.

BACKGROUND: The primary aim of this study was to obtain more knowledge about the occurrence of Cryptosporidium and Giardia in young dogs in Norway. The occurrence of these parasites was investigated in a longitudinal study by repeated faecal sampling of dogs between 1 and 12 months of age (litter samples and individual samples). The dogs were privately owned and from four large breeds. Individual faecal samples were collected from 290 dogs from 57 litters when the dogs were approximately 3, 4, 6, and 12 months old. In addition, pooled samples were collected from 43 of the litters, and from 42 of the mother bitches, when the puppies were approximately 1 and/or 2 months old. METHODS: The samples were purified by sucrose gradient flotation concentration and examined by immunofluorescent staining. RESULTS: 128 (44.1%) of the young dogs had one or more Cryptosporidium positive samples, whilst 60 (20.7%) dogs had one or more Giardia positive samples. The prevalence of the parasites varied with age. For Cryptosporidium, the individual prevalence was between 5.1% and 22.5%, with the highest level in dogs < 6 months old, and declining with age. For Giardia, the individual prevalence was between 6.0% and 11.4%, with the highest level in dogs > 6 months old, but the differences between age groups were not statistically significant. Significant differences in prevalences were found in relation to geographic location of the dogs. Both parasites occurred at low prevalences in Northern Norway. CONCLUSION: Both Cryptosporidium and Giardia are common in Norwegian dogs, with Cryptosporidium more prevalent than Giardia. Prevalences of the parasites were found to be influenced by age, geographical location, and infection status before weaning.

Cryptosporidium oocysts: challenging adversaries?

A recent review by Brendon King and Paul Monis once again puts Cryptosporidium oocysts under the spotlight. Why is this tough transmission stage so troublesome now? And are future environments likely to assist or hinder its apparent ubiquity? Here, we explore further the fascination and challenge engendered by this parasite transmission stage.

Occurrence of Giardia and Cryptosporidium in Norwegian red foxes (Vulpes vulpes).

Faecal samples from 269 Norwegian wild red foxes (Vulpes vulpes) shot during the hunting season (October-April) in 2002-2004 were examined for the presence of Giardia and Cryptosporidium. Cryptosporidium oocysts were detected in samples from 6 (2.2%) of the foxes, and Giardia cysts in 13 (4.8%) of the foxes. The prevalence of Giardia infection was significantly higher in juvenile male foxes than in adult male foxes, but no other significant differences between age and sex were found. No significant differences in prevalence related to geographical origin of animals were found. Insufficient nucleated Cryptosporidium oocysts were isolated for successful PCR, but genotyping of Giardia duodenalis isolates from seven foxes demonstrated a high degree of heterogeneity amongst them, with all isolates belonging to the zoonotic Assemblages A and B.

Removal of parasitic protozoa from water using a mobile water filtration apparatus intended for field use by military or emergency personnel.

Approximately 3,200 L of water were inoculated with Cryptosporidium oocysts and Giardia cysts. The contaminated water was filtered through a Berkefeld Filter, type TWA 3, as used by the Norwegian army. Nine 10-L samples of filtered water and four 10-L control samples of unfiltered water were analyzed for Cryptosporidium oocysts and Giardia cysts. On average, 1,920 Giardia cysts and 2,735 Cryptosporidium oocysts per 10 L were detected in the unfiltered water. Between 0 and 2 Giardia cysts and between 2 and 47 Cryptosporidium oocysts were detected in the filtered 10-L samples. Percentage removal of parasites was between 99.8 and > 99.95% for Giardia (2.98 to > 3.28 log removal) and between 98.3 and 99.9% (1.76-3.14 log removal) for Cryptosporidium. The Berkefeld filter TWA 3 complies with published recommendations for municipal water supply for removal of Giardia but does not always comply with such regulations for Cryptosporidium.

Microbiological analysis of seed sprouts in Norway.

As part of larger survey of microbial contamination of fruits and vegetables in Norway, four different sprouted seed products were analysed for bacterial and parasitic contaminants (n = 300 for bacterial analyses and n = from 17 to 171 for parasite analyses, depending on parasite). Escherichia coli O157, Salmonella, Listeria monocytogenes, Cyclospora oocysts, Ascaris eggs and other helminth parasites were not detected in any of the sprout samples. Thermotolerant coliform bacteria (TCB) were isolated from approximately 25% of the sprout samples, with the highest percentage of TCB positive samples in alfalfa sprouts. Most TCB were Enterobacter spp. and Klebsiella. E. coli was isolated from 8 of 62 TCB positive mung bean sprout samples. Cryptosporidium oocysts were detected in 8% of the sprout samples and Giardia cysts were detected in 2% of the samples. All the Cryptosporidium positive samples, and most of the Giardia positive samples, were mung bean sprouts. Parasite concentrations in positive samples were low (between 1 and 3 oocysts/cysts per 50 g sprouts). Sprout irrigation water was also analysed for microbial contaminants. E. coli O157 and L. monocytogenes were not detected. TCB were isolated from approximately 40% of the water samples. Salmonella reading was isolated from three samples of spent irrigation water on 3 consecutive days. Cryptosporidium and Giardia were also isolated from spent irrigation water. Additionally, eight samples of unsprouted mung bean seed were analysed for Cryptosporidium oocysts and Giardia cysts. One or both of these parasites were detected in six of the unsprouted seed samples at concentrations of between 1 and 5 oocysts/cysts per 100 g unsprouted seed. Whilst our results support spent irrigation water as the most suitable matrix for testing for bacteria, unsprouted seed is considered the more useful matrix for analysing for parasite contaminants.