RESUMO
In our study, we estimated the frequency of haplotype for cholesterol deficiency (HCD) carriers in the Russian Holstein cattle population. We studied 1817 random samples of cows born in 2010-2017 from ten herds and 331 cows and heifers from the other three herds born in 2016-2019, fathers or fathers of mothers of which were HCD carriers. The method of AS-PCR was used for animals genotyping. In the first group of animals, the incidence of HCD carriers was 8.09%, and in the second one - 23.26%. Our results demonstrated the necessity to test cows for the carriage of the HCD genetic defect in the Russian population of Holstein cattle.
Assuntos
Doenças dos Bovinos/genética , Colesterol/deficiência , Predisposição Genética para Doença , Haplótipos , Animais , BovinosRESUMO
This is the first report performing the whole genome SNP scanning of snow sheep (Ovis nivicola). Samples of snow sheep (n = 18) collected in six different regions of the Republic of Sakha (Yakutia) from 64° to 71° N. For SNP genotyping, we applied Ovine 50K SNP BeadChip (Illumina, United States), designed for domestic sheep. The total number of genotyped SNPs (call rate 90%) was 47796 (88.1% of total SNPs), wherein 1006 SNPs were polymorphic (2.1%). Principal component analysis (PCA) showed the clear differentiation within the species O. nivicola: studied individuals were distributed among five distinct arrays corresponding to the geographical locations of sampling points. Our results demonstrate that the DNA chip designed for domestic sheep can be successfully used to study the allele pool and the genetic structure of snow sheep populations.
Assuntos
Polimorfismo de Nucleotídeo Único , Ovinos/genética , Animais , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Filogeografia , Federação Russa , Especificidade da EspécieRESUMO
A comparative study of the informativeness of SNP and STR markers for interspecific and intraspecific differentiation of the two species of the genus Ovis, snow sheep (O. nivicola) and domestic sheep (O. aries), was conducted. Eleven STR loci combined into two multiplex panels were examined. SNP analysis was performed with the DNA microarray OvineSNP50K BeadChip featuring 54241 SNPs. The possibility of clear differentiation of the studied Ovis species with both types of genetic markers was demonstrated. The advantages of SNP markers for intraspecific differentiation of the O. aries breeds and O. nivicola geographical groups were revealed. The areas of application of the studied types of DNA markers are discussed.
Assuntos
Marcadores Genéticos , Repetições de Microssatélites/genética , Polimorfismo de Nucleotídeo Único/genética , Carneiro Doméstico/genética , Animais , Filogenia , Especificidade da EspécieRESUMO
Karyotypes of the hybrid cell lines NS-RL-3 (TK- -sheep kidney cells and rabbit lymphocytes) and betaCR-NS (TK- -rabbit beta-cells and TK- -sheep kidney cells) were investigated. It was shown that both hybrid cell lines were characterized by presence of both sheep and rabbit chromosomes, which number and structure varies depending on the cell type and the number of passages. In some cases the aberrant chromosomes were identified. It was observed, that 40-50% of the NS-RL-3 cells survived in culture in the presence of the human blood serum, and also were identified during 7-28 days after their introduction into the organism of the animal. Thus, the partial immunological tolerance of the hybrid cell lines has been suggested.
