SERS of cylindrospermopsin cyanotoxin: Prospects for quantitative analysis in solution and in fish tissue.

Cylindrospermopsin (CYN), a cyanotoxin occurring in environmental waters as a cyanobacteria metabolite, has recently raised increased interest both in the scientific community and the environmental, food control and health care bodies due to the incidence of poisoning reports and the lack of prompt, effective detection and monitoring techniques. Here we report comprehensive Raman and SERS spectroscopy data on CYN cyanotoxin and provide a detailed characterization of the vibrational Raman signal based on DFT calculation as well as the adsorption properties with respect to the silver nanoparticles surface. Quantitative SERS analysis was achieved for concentrations range from 0.218 nM to 2.18 µM in aqueous solution. We further investigated the SERS discrimination of artificially intoxicated fish tissue from normal one, using linear discriminant analysis. Significant changes in SERS signal of toxic tissue compared to normal one allowed clear and fast differentiation of toxic tissue with 100% specificity/sensitivity. The cross-validation procedure provided 100% clear separation based on the SERS data. The results open reliable perspectives for SERS monitoring the environmental water bodies.

The aquaculture supply chain in the time of covid-19 pandemic: Vulnerability, resilience, solutions and priorities at the global scale.

The COVID-19 global pandemic has had severe, unpredictable and synchronous impacts on all levels of perishable food supply chains (PFSC), across multiple sectors and spatial scales. Aquaculture plays a vital and rapidly expanding role in food security, in some cases overtaking wild caught fisheries in the production of high-quality animal protein in this PFSC. We performed a rapid global assessment to evaluate the effects of the COVID-19 pandemic and related emerging control measures on the aquaculture supply chain. Socio-economic effects of the pandemic were analysed by surveying the perceptions of stakeholders, who were asked to describe potential supply-side disruption, vulnerabilities and resilience patterns along the production pipeline with four main supply chain components: a) hatchery, b) production/processing, c) distribution/logistics and d) market. We also assessed different farming strategies, comparing land- vs. sea-based systems; extensive vs. intensive methods; and with and without integrated multi-trophic aquaculture, IMTA. In addition to evaluating levels and sources of economic distress, interviewees were asked to identify mitigation solutions adopted at local / internal (i.e., farm-site) scales, and to express their preference on national / external scale mitigation measures among a set of a priori options. Survey responses identified the potential causes of disruption, ripple effects, sources of food insecurity, and socio-economic conflicts. They also pointed to various levels of mitigation strategies. The collated evidence represents a first baseline useful to address future disaster-driven responses, to reinforce the resilience of the sector and to facilitate the design reconstruction plans and mitigation measures, such as financial aid strategies.

Reproductive cycles of Mugil cephalus, Liza ramada and Liza aurata (Teleostei: Mugilidae).

The reproductive cycles of three mullet species from the Eastern Adriatic coast were described using several biological parameters (gonado-somatic index, oocyte diameter and sex ratio) to improve knowledge about their reproduction.