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2.
AORN J ; 112(4): 345-352, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32990961

RESUMO

Retained items (eg, sponges, sharps) after surgical procedures are reportable errors that can result in patient harm or death and increased patient and health care system costs. Perioperative use of radiofrequency (RF) technology may decrease the number of retained sponges and reduce hospital costs. We sought to determine whether the use of RF technology may be associated with fewer retained sponges, improved patient outcomes, and decreased hospital costs. We completed a retrospective evaluation of incident reports before and after implementing the use of an RF system for retained surgical sponges. We found that using RF technology was associated with fewer retained sponges and improved outcomes at our facility. We also determined that mortality rates before and after RF technology implementation were similar, and we estimated that our hospital's costs were reduced.


Assuntos
Corpos Estranhos , Tampões de Gaze Cirúrgicos , Corpos Estranhos/prevenção & controle , Humanos , Erros Médicos , Ondas de Rádio , Estudos Retrospectivos , Tecnologia
3.
Am J Surg ; 219(3): 486-489, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31582177

RESUMO

BACKGROUND: The purpose of this study was to identify the frequency, causes and estimated cost of first case operating room (OR) delays. METHOD: A quarterly prospective review of the first cases in the OR was completed in 2018. The frequency and causes for delays were determined. Median delay time was calculated and opportunity cost was estimated based on idle labor and overtime for staffing of rooms beyond scheduled end times. RESULTS: Of 3604 first cases performed, 55% were delayed for a median 12 min (IQR 6-24 min). The patient and surgeon were responsible for 50% of the causes. Orthopedic (20%) and General (18%) Surgery accounted for the greatest percentage of total delay. A loss of 631 h resulted in an estimated cost of $311,966 for idle labor and $78,623 for nursing overtime. CONCLUSION: Improving accountability and reducing patient-related delays will have the greatest impact on reducing first case on-time delays.


Assuntos
Eficiência Organizacional , Salas Cirúrgicas/organização & administração , Admissão e Escalonamento de Pessoal , Agendamento de Consultas , Custos e Análise de Custo , Humanos , Ohio , Salas Cirúrgicas/economia , Admissão e Escalonamento de Pessoal/economia , Estudos Prospectivos , Fatores de Tempo
4.
Exp Parasitol ; 149: 24-31, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25500215

RESUMO

Babesia spp. are obligate protozoan parasites of red blood cells. Transmission to humans occurs through bites from infected ticks or blood transfusion. Infections with B. microti account for the majority of the reported cases of human babesiosis in the USA. A lower incidence is caused by the more recently described species B. duncani. The current gold standard for detection of Babesia is microscopic examination of blood smears. Recent PCR-based assays, including real-time PCR, have been developed for B. microti. On the other hand, molecular assays that detect and distinguish between B. microti and B. duncani infections are lacking. Closely related species of Babesia can be differentiated due to sequence variation within the internal transcribed spacer (ITS) regions of nuclear ribosomal RNAs. In the present study, we targeted the ITS regions of B. microti and B. duncani to develop sensitive and species-specific droplet digital PCR (ddPCR) assays. The assays were shown to discriminate B. microti from B. duncani and resulted in limits of detection of ~10 gene copies. Moreover, ddPCR for these species were useful in DNA extracted from blood of experimentally infected hamsters, detecting infections of low parasitemia that were negative by microscopic examination. In summary, we have developed sensitive and specific quantitative ddPCR assays for the detection of B. microti and B. duncani in blood. Our methods could be used as sensitive approaches to monitor the progression of parasitemia in rodent models of infection as well as serve as suitable molecular tests in blood screening.


Assuntos
Babesia/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Animais , Babesia/classificação , Babesia/genética , Babesia microti/classificação , Babesia microti/genética , Babesia microti/isolamento & purificação , Sequência de Bases , Cricetinae , DNA Intergênico/química , DNA de Protozoário/sangue , DNA Ribossômico/química , Mesocricetus , Dados de Sequência Molecular , RNA Ribossômico 18S/genética , RNA Ribossômico 28S/genética , RNA Ribossômico 5,8S/genética , Reação em Cadeia da Polimerase em Tempo Real , Sensibilidade e Especificidade
5.
J Eukaryot Microbiol ; 58(1): 75-6, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21182562

RESUMO

We describe the isolation of a mycobacterium from Acanthamoeba castellanii strain Ma (ATCC(®) 50370(™)). The mycobacterium resides within vacuoles of A. castellanii, can be cultured by routine methodologies, and is a member of the Mycobacterium avium complex. Previously unrecognized mycobacterial endosymbionts are likely common among strains of Acanthamoeba housed at culture collections.


Assuntos
Acanthamoeba castellanii/microbiologia , Mycobacterium/fisiologia , Simbiose , Acanthamoeba castellanii/fisiologia , Mycobacterium/genética , Mycobacterium/isolamento & purificação
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