RESUMO
In recent years, DISC1 has emerged as one of the most credible and best supported candidate genes for schizophrenia and related neuropsychiatric disorders. Furthermore, increasing evidence--both genetic and functional--indicates that many of its protein interaction partners are also involved in the development of these diseases. In this study, we applied a pooled sample 454 sequencing strategy, to explore the contribution of genetic variation in DISC1 and 10 of its interaction partners (ATF5, Grb2, FEZ1, LIS-1, PDE4B, NDE1, NDEL1, TRAF3IP1, YWHAE, and ZNF365) to schizophrenia susceptibility in an isolated northern Swedish population. Mutation burden analysis of the identified variants in a population of 486 SZ patients and 514 control individuals, revealed that non-synonymous rare variants with a MAF<0.01 were significantly more present in patients compared to controls (8.64% versus 4.7%, Pâ=â0.018), providing further evidence for the involvement of DISC1 and some of its interaction partners in psychiatric disorders. This increased burden of rare missense variants was even more striking in a subgroup of early onset patients (12.9% versus 4.7%, Pâ=â0.0004), highlighting the importance of studying subgroups of patients and identifying endophenotypes. Upon investigation of the potential functional effects associated with the identified missense variants, we found that â¼90% of these variants reside in intrinsically disordered protein regions. The observed increase in mutation burden in patients provides further support for the role of the DISC1 pathway in schizophrenia. Furthermore, this study presents the first evidence supporting the involvement of mutations within intrinsically disordered protein regions in the pathogenesis of psychiatric disorders. As many important biological functions depend directly on the disordered state, alteration of this disorder in key pathways may represent an intriguing new disease mechanism for schizophrenia and related neuropsychiatric diseases. Further research into this unexplored domain will be required to elucidate the role of the identified variants in schizophrenia etiology.
Assuntos
Predisposição Genética para Doença , Mutação de Sentido Incorreto/genética , Proteínas do Tecido Nervoso/genética , Esquizofrenia/genética , Análise de Sequência de DNA/métodos , Transdução de Sinais/genética , Adulto , Idade de Início , Estudos de Casos e Controles , Biologia Computacional , Análise Mutacional de DNA , Mutação da Fase de Leitura/genética , Frequência do Gene/genética , Genética Populacional , Humanos , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Esquizofrenia/epidemiologia , Suécia/epidemiologia , Adulto JovemRESUMO
We evaluated multiplex PCR amplification as a front-end for high-throughput sequencing, to widen the applicability of massive parallel sequencers for the detailed analysis of complex genomes. Using multiplex PCR reactions, we sequenced the complete coding regions of seven genes implicated in peripheral neuropathies in 40 individuals on a GS-FLX genome sequencer (Roche). The resulting dataset showed highly specific and uniform amplification. Comparison of the GS-FLX sequencing data with the dataset generated by Sanger sequencing confirmed the detection of all variants present and proved the sensitivity of the method for mutation detection. In addition, we showed that we could exploit the multiplexed PCR amplicons to determine individual copy number variation (CNV), increasing the spectrum of detected variations to both genetic and genomic variants. We conclude that our straightforward procedure substantially expands the applicability of the massive parallel sequencers for sequencing projects of a moderate number of amplicons (50-500) with typical applications in resequencing exons in positional or functional candidate regions and molecular genetic diagnostics.
Assuntos
Dosagem de Genes , Mutação , Reação em Cadeia da Polimerase/métodos , Análise de Sequência de DNA/métodos , Doença de Charcot-Marie-Tooth/diagnóstico , Doença de Charcot-Marie-Tooth/genética , Conexinas/genética , Proteína 2 de Resposta de Crescimento Precoce/genética , GTP Fosfo-Hidrolases , Predisposição Genética para Doença/genética , Variação Genética , Humanos , Proteínas de Membrana/genética , Proteínas Mitocondriais/genética , Proteína P0 da Mielina/genética , Proteínas da Mielina/genética , Proteínas de Neurofilamentos/genética , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Análise de Sequência de DNA/instrumentação , Proteína beta-1 de Junções ComunicantesRESUMO
In this omics era, researchers in biosciences need more than ever user-friendly, fast, and straightforward computational tools for high-throughput research. In this chapter, we present SNPbox and explain the general primer design strategy. We also show how the four modules, Exon, Single-Nucleotide Polymorphism (SNP), Saturation, and Exon + Saturation, exactly apply the primer design strategy, give clear guidance for users of the Web interface at http://www.SNPbox.org, and explain how pre-designed primers for Ensembl genes can be visualized and retrieved.