1.
Mol Gen Genet
; 214(2): 213-7, 1988 Oct.
Artigo
em Inglês
| MEDLINE
| ID: mdl-2853291
RESUMO
Derivatives of the broad-host-range plasmid RP4, containing the wild-type or modified transposon Tn5 were transferred by conjugation to various Stigmatella aurantiaca isolates. The transposons and in some cases fragments of the plasmid as well were integrated into the chromosome. Thus, insertional mutants have been obtained affected in spore formation in liquid culture.
Assuntos
Elementos de DNA Transponíveis , Mutação , Myxococcales/genética , Plasmídeos , Southern Blotting , DNA Bacteriano/genética , Myxococcales/fisiologia , Hibridização de Ácido Nucleico , Mapeamento por Restrição , Esporos Bacterianos/fisiologia
2.
Histochemistry
; 83(3): 251-5, 1985.
Artigo
em Inglês
| MEDLINE
| ID: mdl-4044310
RESUMO
We demonstrated that alkaline phosphatase was localized on the cell membrane of Dictyostelium discoideum amebae and on isolated plasma membranes. The enzyme activity was specifically inhibited by 0.01 M KCN or cysteine. The same method could also be applied to baker's yeast and MDCK cells (dog kidney cells in vitro).
