[The time course of experimentally altered thyroid status manifestations in female C3H-A mice].

The female C3H-A mice with agouty fur color were used to model hyper- and hypothyroidism in the long lasting experiment. The study was carried out for 44 weeks. Hyperthyroidism was induced by the administration of the L-thyroxine injections on alternate days during the whole period of the investigation. Hypothyroidism was achieved by adding propylthiouracil to the drinking water. The change of thyroid state was characterized by biphasic change in body weight. At the beginning of the experiment the hypothyroid animals were retarding by their weight. Otherwise the hyperthyroid animals were advancing by their weight. But since the 18th-21st week the initial trends changed, i. e. the hypothyroid mice body weight started ahead the hyperthyroid one. In the open field test both hypo- and hyperthyroid animals demonstrated the higher level of the investigating activity in comparison with the euthyroid mice. In the hyperthyroid mice the frequency of side-activity acts (grooming) increased significantly. Thus, the hyperthyroid animals appeared to be more anxious. To the 18th week of the experiment the animals of study groups started to demonstrate the apparent visual difference in their fur color. The hyperthyroid mice fur color became darker than one of the hypothyroid and the euthyroid mice. It is worthy of note that the hyperthyroid mice fur color was getting lighter than one of the euthyroid animals. The results are discussed in the context of hypothalamic-pituitary-thyroid axis functioning. The possible mechanisms of hormonal regulation of the fur color in mice are considered to include the hypothalamic-pituitary-thyroid axis hormones activities.

The incidence of breast tumor during experimental hyperthyroidism.

The incidence of breast tumor in inbred C3H-A mice (virgin female) from hyperthyroid, hypothyroid, and euthyroid control groups at week 44 of the experiment was 86.67, 30, and 62.5%, respectively. The percentage of metastatic lymph nodes in these groups was 65.9±16.7, 35.9±5.9, and 53.9±7.2%, respectively (p<0.005). It was shown that hyperthyroidism was associated with increased incidence and aggressiveness of breast tumor, while hypothyroidism was associated with lower incidence of breast tumor in comparison with the control.

[Clinical and immunological efficiency of sodium nucleospermate in treating chronic endometritis and infertility complicated by HPV infection].

Clinical efficacy of sodium nucleospermate (SNS) has shown in the treatment of 26 female patients with chronic endometritis, unsuccessful attempts at in vitro fertilization, and persistent viral infection. Cells from endometrium were processed by liquid cytology and studied by immunocytochemistry techniques. It has been clearly established that, after SNS treatment, the frequency of CD20- and CD56-positive cells was significantly decreased (p < 0.05) and the frequency of TLR4 and TLR9 expressing cells in endometrium has been significantly improved (p < 0.05). In addition, the human papilloma virus (HPV) load was reduced as manifested by the real-time PCR test. The obtained results show good prospects for successful use SNS as a means of pathogenetic therapy in patients with this diagnosis.