[The treatment of thermal skin burns by using cultured fibroblasts]. / Lechenie termicheskikh ozhogov kozhi s ispol'zovaniem kul'tivirovannykh fibroblastov.

Transplants of monolayer culture of human allofibroblasts is characterized morphologically, the experience of their use in the treatment of 184 patients with burns is summarized. Fibroblasts of the transplant are shown to be able to proliferate and to synthetize an extracellular matrix. Their use allowed reducing significantly the time of marginal burn epithelialization, and the epithelialization of donor wounds with long healing and that of deep burns when fibroblast culture transplantation is combined with 1:6 autotransplantation. Rapid recovery of the skin is provided by a transplanted fibroblast stimulation of the proliferation of the granulation tissue pericytes, its fibroblasts and autoepidermocytes.

[Use of cell cultures in the local treatment of burn wounds]. / Ispol'zovanie kletochnykh kul'tur pri mestnom lechenii ozhogovykh ran.

The article deals with the results of using cultivated allofibroblasts in topical treatment of burn wounds. Allofibroblasts obtained from the derma of burnt individuals were transplanted to the burn wounds of 28 patients. In 12 among 15 patients (80%) with IIIa-IIIb degree burns transplantation induced epithelialization of the burns in 12.1 +/- 2.4 days. Thirteen patients had deep burns, only in 3 of them with a circumscribed burn area transplantation proved effective. Complications of transplantation were encountered in 5 patients--rejection of the transplant in 2 and its suppuration in 3 patients. Allofibroblast transplantation may be applied as an independent method of treatment in IIIa, b degree burns.

[The effect of the extracellular matrix on the adhesion and proliferation of epidermocytes in culture]. / Vliianie ékstratselliuliarnogo matriksa na adgeziiu i proliferatsiiu c épidermotsitov v kul'ture.

The influence of types I, III collagen and fibronectin on cultured epidermocytes was studied. The extracellular matrix in vitro stimulated not only adhesion, but also cell proliferation. The highest degree of epidermocytic proliferation on fibronectin matrix was observed.

[Treatment of chronic non-healing donor sites: transplantation of cultured human fibroblasts]. / Lechenie dlitelno ne zazhivaiushchikh donorskikh uchastkov: transplan tatsiia kultivirovannykh allofibroblastov cheloveka.

The authors discuss the first results of the use of cultivated human allofibroblasts in the treatment of persisting wounds of the donor areas in 13 patients with deep burns and cicatricial deformities caused by the burns. Transplantation of cell cultures led to complete epithelialization of the wounds in 6-10 days. Complication, suppuration of the grafted material, was recorded in one case. Reaction of graft rejection was not encountered. The authors believe that the effect of the transplantation is due to stimulation of the epithelialization processes by the cultivated fibroblasts.

[The involvement and reparative regeneration of the mucosa of the airways in thermal inhalation trauma in burn patients]. / Porazhenie i reparativnaia regeneratsiia slizistoi obolochki vozdukhonosnykh putei pri termoingaliatsionnoi travme u obozhzhennykh.

The adaptive reorganization in mucosa of the upper airways was investigated in 15 burned patients during the first 24 days postburn using historadiography in vitro. The fibreoptic bronchoscopic biopsy specimens obtained in vivo were analysed. The initial significant destructive changes in mucosa and inhibition of DNA and RNA synthesis in the epitheliocytes were changed at day 8 postburn by the development of consecutively arising regenerative processes. The intensification levels of proliferative and metabolic reactions obtained under conditions of the serial bronchoscopies represent important diagnostic and prognostic criteria.

[Cultured human skin cells in the treatment of burns]. / Lechenie ozhogov s ispolzovaniem kultivirovannykh kletok kozhi cheloveka.

The work discusses the results of experience of long-year's standing of the staff of the Vishnevsky Institute of Surgery in the creation of a biological bandage with the use of cultivated human skin cells. A graft of cultivated human allofibroblasts was used for closure of burn wounds for the first time. Its efficacy was studied in 86 cases of burns. The use of cultivated allofibroblasts is indicated in bordering III ab degree burns and sluggishly healing donor areas formed after autodermoplasty. Study of the efficacy of cultivated allofibroblasts in the treatment of deep burns in combination with dermoplasty with a 1:6 perforated skin autograft are continued. The low cost and technical simplicity of the method allow cultivated fibroblast grafts to be recommended for wide clinical practice.

[Allotransplantation of cultured fibroblasts on non-healing wounds after autodermatoplasty]. / Allotransplantatsiia kul'tivirovannykh fibroblastov na nezazhivaiushchie rany posle autodermoplastiki.

An allograft from man cultured fibroblasts has been received. Results of its transplantation on long not-healing donor wounds in 13 patients after autodermatoplastic operations have been studied. Expressed stimulating influence of cultured fibroblasts on donor wound epithelialization processes has been stated. In spite of allograft applications there has been no tearing away reactions. It has been connected with elimination of superficial cell antigens during cultivation. Microbe contamination of more than 10(2) is the contraindication for grafting.

[Cloning and expression of the gene for thermostable pullulanase from Clostridium thermohydrosulfuricum in Escherichia coli]. / Klonirovanie i ékspressiia gena termostabil'noi pullulanazy Clostridium thermohydrosulfuricum v kletkakh Escherichia coli.

Using a pUC19-based genomic library of the anaerobic thermophilic bacterium C. thermohydrosulfuricum a DNA fragment that confers pullulanase activity to E. coli cells has been identified. Subcloning and restriction mapping procedures was carried out and the primary structure of the 5'-region of the pullulanase gene (pul) was determined. The pul enzyme was shown to be a protein with molecular weight of approximately 60,000. It was found that both pullulanase and glucoamylase activities resides in pullulanase. The intracellular distribution of pullulanase was studied. An E. coli strain that produces large amounts of thermostable pullulanase has been constructed.

[Isolation and characteristics of thermostable pullulanase from Clostridium thermohydrosulfuricum produced by Escherichia coli cells]. / Vydelenie i kharakteristika termostabil'noi pullulanazy Clostridium thermohydrosulfuricum, produtsiruemoi kletkami Escherichia coli.

The expressed gene (pul) for a thermostable pullulanase from Clostridium thermohydrosulfuricum was cloned into Escherichia coli. The enzyme was purified from cell extracts of E. coli by thermoinactivation, ammonium sulphate precipitation and gel exclusion. The purified enzyme was characterized as monomer with both pullulanase and glucoamylase activities. The general physico-chemical and catalytic properties of this enzyme were obtained. In particular, pullulanase and glucoamylase activities were stable and optimally active at 65 degrees C. The pH optimum for activity was 5.8. The amino acid composition and amino acid sequence of N-terminal end were estimated.

[Use of cultured fibroblasts in the treatment of burn wounds]. / Ispol'zovanie kul'tivirovannykh fibroblastov pri lechenii ozhogovykh ran.

This paper deals with the possibility of closing of burn surfaces of skin wounds with cultivation of patients' fibroblasts. Transplantation of cultured fibroblasts was performed in 11 patients with 30-75% of a body surface burned. The results obtained indicate a prospect of this method of burn treatment.