Non-Linear Association of Exercise with Animal Protein Consumption among U.S. Adults.

Supply chain disruptions, human and animal health concerns, and environmental impacts of livestock production have spurred renewed attention to animal protein consumption in the U.S. Prior research has sought to better understand animal protein consumption by assessing demographic and socioeconomic determinants. However, physical exercise represents a behavioral determinant of consumption that is societally important and, thus far, has not been considered in empirical assessments. Our objective was to quantify the association of exercise with the consumption of total protein, beef, pork, poultry, seafood, eggs, and dairy among U.S. adults. We analyzed 24 h dietary recall and physical activity data from adults in the 2007-2018 National Health and Nutrition Examination Survey (NHANES). The association of exercise with protein consumption (total and disaggregated animal protein) was assessed using ordinary least squares and multivariate Tobit regression. Non-linear associations of exercise with total protein consumption were found, with the magnitude of association highest at 121-180 min per day of exercise. Non-linear associations were also found with animal protein consumption, which differed in sign and magnitude across protein sources. The magnitudes of association, paired with a sizable share of the study sample engaged in exercise, suggest a substantial influence of exercise on protein consumption habits in the U.S.

Egg producer attitudes and expectations regarding the transition to cage-free production: a mixed-methods approach.

Several cage-free egg mandates and retailer pledge deadlines are set to take effect in January 2026. Yet it is unknown whether producers can transition to cage-free production at a rate commensurate with these goals. This study uses qualitative and quantitative data from 2 U.S. egg producer surveys to evaluate the operational activities of conventional and cage-free facilities, identify market challenges, and assess the expected transition timeline. Across both studies, producers indicated higher fixed and variable costs in cage-free housing systems, with capital and labor being 2 of the central drivers of the cost increase. While several producers are willing to adopt cage-free production, they are hesitant to view the market shift as an opportunity. Among the most commonly cited barriers are limited customer demand, high capital costs, and a contradiction to environmental sustainability and food security efforts. With the current challenges, respondents are skeptical that the industry will meet the January 2026 voluntary pledge deadlines. The results from this study offer a holistic view of the potential ramifications of the cage-free transition on the egg market and can be used to inform marketing strategies and policy discussions.

Regional and plant-size impacts of COVID-19 on beef processing.

During the COVID-19 pandemic, U.S. consumers witnessed changes in the volume and type of meat products available at retail and food service markets. Simultaneously, widening farm-to-wholesale price spreads fueled calls for industry change and several related policy proposals. The objective of this study is to document fed cattle slaughter and evaluate the structure and performance of the beef processing industry during the early stages of the COVID-19 pandemic. For major beef-producing regions, the 2019-2020 change in federally inspected U.S. cattle slaughter volumes varied in isolated instances with regional reliance on larger processing facilities. Implications of this are discussed both for current policy and industry discussions, as well as to encourage additional future research.

U.S. perspective: Meat demand outdoes meat avoidance.

Despite ample discussion of health, environment, and animal welfare effects of meat production and consumption, this article documents past, current, and projected consumption patterns reflecting robust meat demand in the United States. There is some evidence of meat avoidance behavior among a segment of the population, including younger, higher educated, higher income consumers in the Western United States. At the same time, the majority of U.S. residents self-declare as regularly consuming products from animals, and there is evidence of strong demand growth for meat products in recent years. Key factors influencing protein purchasing decisions are presented revealing critical roles of taste, freshness, and safety. Combined this article summarizes both the aggregate and more refined, household-level situation underlying robust meat demand in the U.S.

U.S. Cattle Producer Adoption of Secure Beef Supply Plan Enhanced Biosecurity Practices and Foot-and-Mouth Disease Preparedness.

The prospect of a foot-and-mouth disease (FMD) outbreak in U.S. livestock populations has motivated the development of the Secure Beef Supply (SBS) Plan, which includes a comprehensive list of enhanced biosecurity practices that aim to prevent FMD transmission and facilitate continuity of business during an outbreak. While FMD poses a serious threat to livestock production in the United States, little is known about producers' uptake of the enhanced biosecurity practices included in the SBS Plan. In this study, we benchmark adoption and feasibility-of-adoption perceptions for U.S. cattle producers. Our results show adoption of the 13 enhanced biosecurity practices is generally low. Especially concerning is the low adoption of the three strongly-recommended pre-outbreak practices-having a biosecurity manager, having a written operation-specific enhanced biosecurity plan, and having a line of separation. Adoption of the pre-outbreak practices is likely low because the benefits of adopting the practices depend on a low probability, uncertain event. That said, producers who have adopted the pre-outbreak practices are more likely to have higher feasibility ratings for the remaining enhanced biosecurity practices, suggesting that adoption of the strongly recommended practices is associated with adoption of all enhanced biosecurity during an FMD outbreak. Complementarity is examined and shows that adoption of the pre-outbreak practices coincides with adoption of the outbreak-specific practices. Taken together, our results suggest that adoption of the strongly recommended pre-outbreak practices could help facilitate a quicker and more effective U.S. cattle industry response to an FMD outbreak in the United States.

Meat Demand Monitor during COVID-19.

Meat products represent a significant share of US consumer food expenditures. The COVID-19 pandemic directly impacted both demand and supply of US beef and pork products for a prolonged period, resulting in a myriad of economic impacts. The complex disruptions create significant challenges in isolating and inferring consumer-demand changes from lagged secondary data. Thus, we turn to novel household-level data from a continuous consumer tracking survey, the Meat Demand Monitor, launched in February 2020, just before the US pandemic. We find diverse impacts across US households related to "hoarding" behavior and financial confidence over the course of the pandemic. Combined, these insights extend our understanding of pandemic impacts on US consumers and provide a timely example of knowledge enabled by ongoing and targeted household-level data collection and analysis.

Beef and Pork Marketing Margins and Price Spreads during COVID-19.

COVID-19-related disruptions led to a historic rise in the spread between livestock and wholesale meat prices. Concerns about concentration and allegations of anticompetitive behavior have led to several inquiries and civil suits by the U.S. Department of Agriculture and the U.S. Department of Justice, with increases in price differentials serving as a focal point. This article notes the difference between price spreads and marketing margins, outlines corresponding economic theory, and describes the empirical evidence on wholesale meat and livestock price dynamics in the wake of COVID-19 disruptions. At one point during the pandemic, beef and pork packers were both operating at about 60% of the previous year's processing volume. We explore how such a massive supply shock would be expected to affect marketing margins even in the absence of anticompetitive behavior. Moreover, we document how margin measurements are critically sensitive to the selection of data and information utilized. Finally, we conclude with some discussion around policy proposals that would pit industry concentration against industry coordination and economies of scale.

Connecting the dots: a comparison of network analysis and exploratory factor analysis to examine psychosocial syndemic indicators among HIV-negative sexual minority men.

Syndemics, or comorbid and mutually reinforcing psychosocial problems, are associated with increased HIV risk among men who have sex with men (MSM). Although the dynamic interplay among syndemic indicators is theorized to be crucial for increasing risk of HIV acquisition, novel approaches are needed to understand how these syndemic problems interrelate. This study examined the associations between nine self-reported syndemic indicators in 194 MSM at high risk of HIV acquisition. We compared exploratory factor analyses (EFA) to a network analysis. In the present study, network analysis consisted of edges representing bidirectional partial polychoric correlations between nodes, which represent psychosocial syndemic indicators. EFA yielded a 1-factor solution including suicidal ideation (SI), injection drug use (IDU), depression, social anxiety, intimate partner violence, substance use, and sexual compulsivity, and excluded heavy drinking and childhood sexual abuse. Network analysis yielded a pattern of interconnectedness with the most central nodes being SI, IDU, substance use, and depression. Statistically significant relationships (absolute edge weights) were found between SI and depression, social anxiety, and IDU, and IDU and substance use. These results suggest that depression and substance use, especially more severe presentations of these conditions such as SI and IDU, are prominent interconnected components of the HIV syndemic among MSM at high risk for HIV acquisition. SI, IDU, substance use, and depression may indeed be prudent targets of intervention. Future research on the inclusion of these syndemic indicators in analytical models involving interaction terms may be warranted.

Will an incentive-compatible indemnity policy please stand up? Livestock producer willingness to self-protect.

This study evaluates the role of private market signals and conditional indemnity policies in livestock producer willingness to self-protect against disease and invest more in biosecurity. Our focus on Tier 1 swine diseases and U.S. hog producer decision-making is timely and informative for a multitude of current disease discussions. We find biosecurity effort adjusts to economic incentives in private, livestock markets and public, indemnity policies.

Adoption of Secure Pork Supply Plan Biosecurity by U.S. Swine Producers.

There is mounting concern about the negative animal health and supply chain consequences of animal disease outbreaks in the United States. Recent disease outbreaks have drawn attention to the need for additional understanding of biosecurity efforts to reduce disease frequency, spread, and impact. Biosecurity is a key component of the Secure Pork Supply (SPS) Plan designed to provide business continuity in the event of a foreign animal disease outbreak as well as help protect operations from endemic diseases. Core biosecurity recommendations outlined in the SPS Plan are a written site-specific biosecurity plan and implementation of a perimeter buffer area and a line of separation. To-date, no benchmarking of SPS Plan biosecurity implementation has been done. Utilizing data from a 2017 survey of U.S. swine producers, this study shows that SPS Plan biosecurity adoption varies and is affected by how feasible producers believe implementation of each biosecurity practice is on their operation. Furthermore, binomial logit regression analyses indicate producer and operation demographics and producer risk attitudes and perceptions affect biosecurity adoption. Conditional probabilities reveal that adoption of biosecurity practices is overwhelmingly complementary, suggesting that one biosecurity practice likely increases marginal efficacy of another biosecurity practice. The insights this study provides regarding the complexities of biosecurity adoption are vitally important to both educators and policy makers.

US Farm Animal Welfare: An Economic Perspective.

The topic of farm animal welfare (FAW) is both complex and controversial, and inherently involves expertise and views from multiple disciplines. This article provides a summary of economic perspectives on FAW issues in the United States. Practices related to FAW can occur through legal, market or voluntary programs. FAW is not a primary driver of US food demand but negative press has industry-wide effects. Aligning FAW supply and demand can be facilitated through labeling, education, and voluntary programs, but all have pros and cons.

United States feedlot operator willingness to pay for disposal capacity to address foreign animal disease risk.

Foreign animal diseases can cause severe and lasting economic impacts to producers, directly and indirectly. Understanding producer investment cost structures can provide industry and policy makers better tools to encourage biosecurity adoption. Consistent with the literature, many factors can contribute to an individual operator's decision to invest in biosecurity based on individual characteristics, perception of disease likelihood, or expected losses associated with a disease event. We used a producer survey and a one-and-one-half bound econometric model to estimate feedlot operator willingness to pay to invest in disposal capacity within the next 3 years. Results indicate an average willingness to pay of $14,310 for a one-time investment in on-farm disposal capacity to address carcass movement restrictions during a disease outbreak. We found several factors that contribute to and explain the heterogeneity between feedlots and their adoption decisions. Primarily, size of the feedlot and death loss rate significantly impact adoption, which both potentially speak to the financial liquidity and investment potential of a feedlot enterprise. While there is no failsafe in disease prevention, these results provide a better understanding for how to study and structure policy and cost structures to incentivize adoption of biosecurity.

Jordan tobacco dependence treatment guidelines: rationale and development.

Jordan, a high tobacco-burden country, has been working to expand its tobacco dependence treatment services and has completed development of its first customized treatment guidelines. Our paper presents the development process for these guidelines. A group of national and international experts was formed and a national situation analysis for tobacco dependence treatment practices and a detailed review of international evidence were conducted. The guidelines were then drafted and reviewed by national, regional and international experts and were official endorsed by the Jordanian Ministry of Health before being launched. The guidelines comprise concise descriptions and practical supplementary flowcharts covering the major elements of general tobacco dependence treatment. These are the first comprehensive Arabic-language guidelines, including a section focusing on waterpipe use, and we believe they are a reliable and useful resource for neighbouring countries seeking to develop similar guidelines.

Assessment of the economic impacts of porcine epidemic diarrhea virus in the United States.

Porcine epidemic diarrhea virus (PEDV), which first emerged in the United States in 2013, spread throughout the U.S. hog population. Limited preemptive knowledge impeded the understanding of PEDV introduction, spread, and prospective economic impacts in the United States. To assess these impacts, this article reviews the timeline of PEDV in the United States and the corresponding impacts. PEDV is a supply-impacting disease and is not demand inhibiting, as pork demand remained strong since PEDV first appeared. Pig losses reached significant levels during September 2013 through August 2014, with the majority of pork production impacts occurring in 2014. PEDV had differing impacts for subsectors of the pork industry. A budget model demonstrates that producers could have had pig losses and decreases in productivity proportionally smaller than price increases, resulting in net returns above what was expected before the major outbreak of PEDV. Previous literature is reviewed to identify the potential main industry beneficiaries of the PEDV outbreaks in the United States. As a result of reduced volumes of available pig and hog supplies, reductions in annual returns likely occurred for packers, processors, distributors, and retailers. In addition, pork consumers who experienced reduced-supply-induced pork-price increases were likely harmed directly by higher prices paid for pork and indirectly as prices of competing meats were also likely strengthened by PEDV. This article also identifies future considerations motivated by the appearance of PEDV in the United States, such as discussions of industry-wide efficiency and competitive advantage, the future role of PEDV vaccines, enhancement in biosecurity measures, and consumer perceptions of food safety and insecurity.

Induction of resistance to Aplidin in a human ovarian cancer cell line related to MDR expression.

Aplidin-resistant IGROV-1/APL cells were derived from the human ovarian cancer IGROV-1 cell line by exposing the cells to increasing concentration of Aplidin for eight months, starting from a concentration of 10 nM to a final concentration of 4 microM. IGROV-1/APL cell line possesses five fold relative resistance to Aplidin. IGROV-1/APL resistant cell line shows the typical MDR phenotype: (1) increased expression of membrane-associated P-glycoprotein, (2) cross-resistance to drugs like etoposide, doxorubicin, vinblastine, vincristine, taxol, colchicin and the novel anticancer drug Yondelis (ET-743). The Pgp inhibitor cyclosporin-A restored the sensitivity of IGROV-1/APL cells to Aplidin by increasing the drug intracellular concentration. The resistance to Aplidin was not due to the other proteins, such as LPR-1 and MRP-1, being expressed at the same level in resistant and parental cell line. The finding that cells over-expressing Pgp are resistant to Aplidin was confirmed in CEM/VLB 100 cells, that was found to be 5-fold resistant to Aplidin compared to the CEM parental cell line.

Sciatic nerve palsy secondary to postoperative haematoma in primary total hip replacement.

Sciatic nerve palsy is a recognised complication of primary total hip replacement. In our unit this complication was rare with an incidence of < 0.2% in the past ten years. We describe six cases of sciatic nerve palsy occurring in 355 consecutive primary total hip replacements (incidence 1.69%). Each of these palsies was caused by post-operative haematoma in the region of the sciatic nerve. Cases, which were recognised early and surgically-evacuated promptly, showed earlier and more complete recovery. Those patients for whom the diagnosis was delayed, and who were therefore managed expectantly, showed little or no recovery. Unexpected pain and significant swelling in the buttock, as well as signs of sciatic nerve irritation, suggest the presence of haematoma in the region of the sciatic nerve. It is, therefore, of prime importance to be vigilant for the features of a sciatic nerve palsy in the early post-operative period as, when recognised and treated early, the injury to the sciatic nerve may be reversed.

Combination of trabectedin and irinotecan is highly effective in a human rhabdomyosarcoma xenograft.

Our objective was to evaluate in vitro and in vivo the effect of the combination of trabectedin (Yondelis, ET-743) and irinotecan (CPT-11) or its major metabolite SN-38 in a human rhabdomyosarcoma cell line. The schedule trabectedin (1 h) followed by irinotecan or SN-38 (24 h) and the opposite sequence (irinotecan or SN-38 24 h followed by trabectedin 1 h) were analyzed in a rhabdomyosarcoma cell line. In vivo studies were conducted with trabectedin and irinotecan at the doses of 0.2 and 20 mg/kg, respectively, simultaneously administered with a q4d x 3 schedule. In vitro studies indicated an overall additive effect [combination index (CI) relatively close to 1.0], with the former schedule slightly superior to the latter (at the IC50 effect levels: CI=0.89 versus 1.07). Neither transcription nor expression of DNA topoisomerase I was affected by trabectedin treatment. In vivo the therapeutic results of the combination were certainly more impressive: trabectedin and irinotecan combination caused a strong and long-lasting effect on tumor growth (tumor volume inhibition=89%, log10 cell kill=1.6), whereas each drug given as a single agent was only marginally active. The discrepancy between the in vitro and in vivo results suggests possible mechanisms involving host cells, other than tumor cells. The striking effects of the combination observed in vivo could be related to a combination of a direct cytotoxic and an anti-inflammatory indirect effect. The very marked and long-lasting effect of the trabectedin and irinotecan combination in vivo suggests a basis for a clinical evaluation in pediatric patients with rhabdomyosarcoma.

Cell interactions with laser-modified polymer surfaces.

The performance of a polymeric biomaterial depends on the bulk and surface properties. Often, however, the suitability of the surface properties is compromised in favour of the bulk properties. Altering the surface properties of these materials will have a profound effect on how cells and proteins interact with them. Here, we have used an excimer laser to modify the surface wettability of nylon 12. The surface treatment is rapid, cost-effective and can cause reproducible changes in the surface structure of the polymers. Polymers were treated with short wavelength ( < 200 nm) UV light. These wavelengths have sufficient photon energy (6.4eV) to cause bond scission at the material surface. This results in a surface reorganisation with incorporation of oxygen. Surface wettability changes were confirmed using contact angle measurements. Cell interactions with the surfaces were examined using 3T3 fibroblast and HUVEC cells. Cells morphology was examined using a confocal laser scanning microscope (CLSM). Cell activity and cell number on the treated nylon were assessed using biochemical assays for up to seven days. Both fibroblasts and endothelial cells initially proliferated better on treated compared with untreated samples. However, over seven days activity decreased for both cell types on the control samples and endothelial cell activity and cell number also decreased on the treated polymer.

Telephone assistance for smoking cessation: one year cost effectiveness estimations.

A randomised trial evaluated the American Cancer Society's telephone counselling service to assist smoking cessation. Counselling nearly doubles a smokers' odds of quitting and maintaining cessation for one year. The estimated cost for each case of maintained smoking cessation attributable to counselling availability is approximately 1300 dollars.