RESUMO
The present study was designed to evaluate the antifungal activity of Azima tetracantha extracts and isolated compound (friedelin) against fungi. Antifungal activity was carried out using broth microdilution method and fractions were collected using (silica gel) column chromatography. The antifungal activity of Azima tetracantha crude extracts and isolated compound (friedelin) were evaluated using the micro dilution method. Hexane extract showed some antifungal activity. The compound also exhibited antifungal activity against tested fungi. The lowest MIC against Trichophyton rubrum (296) was 62.5 microg/ml and the MIC for Curvularia lunata was 62.5 microg/ml. These results suggest that Friedelin is a promising antifungal agent.
Assuntos
Antifúngicos , Fungos/efeitos dos fármacos , Extratos Vegetais/química , Folhas de Planta/química , Salvadoraceae/química , Triterpenos , Antifúngicos/isolamento & purificação , Antifúngicos/farmacologia , Testes de Sensibilidade Microbiana , Salvadoraceae/anatomia & histologia , Trichophyton/efeitos dos fármacos , Triterpenos/isolamento & purificação , Triterpenos/farmacologiaRESUMO
The present study reports the identification of human sex hormone binding globulin (SHBG)-interacting proteins in the brain using a phage display-based screening technology. Phage display is a system in which a foreign protein is displayed on the surface of a bacteriophage as a fusion protein with one of the coat proteins of the bacteriophage. T7 phage clones expressing normal human brain proteins (human normal brain phage-display cDNA expression library) were screened using SHBG as bait. The bound phage clones were then identified by DNA sequencing and by BLAST search analysis. Of the twenty binding proteins analyzed, three were found to be membrane-associated proteins: synaptosomal associated protein 25 (SNAP25), Thy-1 cell surface antigen and zonadhesin. Further studies will determine if the interactions of SHBG with these proteins have any role in the internalization and cell signaling events or whether they contribute to steroid delivery to specific cells.
Assuntos
Encéfalo/metabolismo , Biblioteca de Peptídeos , Proteínas/metabolismo , Globulina de Ligação a Hormônio Sexual/metabolismo , Bacteriófago T7/genética , Bacteriófago T7/metabolismo , Humanos , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Reação em Cadeia da Polimerase , Ligação Proteica/genética , Proteínas/genética , Proteína 25 Associada a Sinaptossoma/genética , Proteína 25 Associada a Sinaptossoma/metabolismo , Antígenos Thy-1/genética , Antígenos Thy-1/metabolismoRESUMO
We have cloned homologues of the mammalian translationally controlled tumor protein (TCTP) from the human filarial parasites Wuchereria bancrofti and Brugia malayi. TCTP genes from B. malayi and W. bancrofti were expressed in a T7 promoter vector as histidine tagged fusion proteins. Both the recombinant B. malayi TCTP (rBm-TCTP) and recombinant W. bancrofti TCTP (rWb-TCTP) have a molecular mass of approximately 28 kDa with the histidine tag. Sequence analyses showed that there is a 98% similarity between the two filarial TCTPs at amino acid levels and are immunologically cross-reactive. Analysis of soluble proteins from various lifecycle stages of B. malayi suggested that the expression of Bm-TCTP might be differentially regulated and occurs in multimeric form. Recombinant TCTP were found to form multimers in solution under non-reducing conditions. The tendency for filarial TCTPs to become multimers was predicted by the presence of the Lupas coiled coil structure in their sequence. Despite the absence of a signal sequence, Bm-TCTP is present abundantly in the excretory/secretions (ES) of microfilariae. Characterization studies showed that both Bm- and Wb-TCTPs are calcium-binding proteins and have histamine-releasing function in vitro. When injected intraperitoneally both the filarial TCTPs induced inflammatory infiltration of eosinophils into the peritoneal cavity of mice suggesting that the filarial TCTPs may have a role in the allergic inflammatory responses associated with filarial infections.
