RESUMO
Bioactive coatings on implants affect osteogenic differentiation of mesenchymal stem cells (MSC). We studied the morphofunctional state of bone marrow MSC cultured on the surface of calcium phosphate coatings on titanium formed by plasma electrolytic oxidation (PEO). The biocompatible properties of the coatings manifested in the absence of the cytotoxic effect on cells. High expression of receptors (CD90, CD29, and CD106), enhanced synthesis of osteocalcin and osteopontin, and changes in surface architectonics of MSC adherent to the samples confirmed osteoinductive properties of the calcium phosphate PEO coating.
Assuntos
Materiais Revestidos Biocompatíveis/farmacologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Titânio/farmacologia , Ligas/química , Ligas/farmacologia , Animais , Adesão Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Materiais Revestidos Biocompatíveis/química , Masculino , Teste de Materiais , Células-Tronco Mesenquimais/fisiologia , Próteses e Implantes , Ratos , Ratos Wistar , Propriedades de Superfície , Titânio/químicaRESUMO
The cellular and molecular aspects of immunological compatibility of modified osteoinductive calcium-phosphate coatings formed on technically pure titanium VT1-0 by the plasma electrolytic oxidation technology are studied. The technique of coating modified the process of human peripheral blood leukocyte activation in vitro. The most pronounced functional changes were observed in neutrophil population during whole blood contact with titanium sample without coating and with coating formed by plasma electrolytic oxidation on titanium alloy. Sealing of the oxidation layer pores by ultradispersed polytetrafluoroethylene leads to reduction of cell activation. Composite coatings with ultradispersed polytetrafluoroethylene applied by the electrophoretic method are characterized by the best biocompatibility. The data demonstrate the possibility of modifying biomaterial surfaces for inflammatory response control.
