RESUMO
The antigenic relationship between the milk fat globule membrane (MFGM) and the hydrophobic fraction of proteose-peptone (HFPP) was demonstrated, using a mono-specific anti-HFPP antibody.
Assuntos
Caseínas/imunologia , Proteínas de Membrana/imunologia , Proteínas do Leite/imunologia , Fragmentos de Peptídeos/imunologia , Animais , Antígenos/imunologia , Caseínas/isolamento & purificação , Bovinos , Cromatografia de Afinidade , Imunodifusão , Proteínas de Membrana/isolamento & purificação , Proteínas do Leite/isolamento & purificação , Peso Molecular , Mucina-1 , Fragmentos de Peptídeos/isolamento & purificação , Soroalbumina Bovina/imunologia , Soroalbumina Bovina/isolamento & purificaçãoRESUMO
Fluorescent antibody was prepared against a temperate-soil isolate of Beijerinckia obtained from a rhizosphere of rice growing in Camargue (France). The antibody did not cross-react with any of 6 species of Azotobacter, 4 species of Beijerinckia, or 44 unidentified soil bacteria isolated from a spectrum of rhizospheres, but strongly stained the homologous Beijerinckia isolate. The isolate grew well in autoclave Camargue soil, but increased in numbers only slightly in nonsterile soil during 9 days. Preliminary examination of rice plants grown in the laboratory in soil from which the Beijerinckia was originally isolated did not show detectable Beijerinckia in the rhizosphere. The fluorescent antibody was sufficiently sensitive and specific to permit more extensive study of Beijerinckia in relation to nitrogen fixation in the rhizospher of rice.
