A role for nonadaptive processes in plant genome size evolution?

Genome sizes vary widely among species, but comprehensive explanations for the emergence of this variation have not been validated. Lynch and Conery (2003) hypothesized that genome expansion is maladaptive, and that lineages with small effective population size (N(e)) evolve larger genomes than those with large N(e) as a consequence of the lowered efficacy of natural selection in small populations. In addition, mating systems likely affect genome size evolution via effects on both N(e) and the spread of transposable elements (TEs). We present a comparative analysis of the effects of N(e) and mating system on genome size evolution in seed plants. The dataset includes 205 species with monoploid genome size estimates (corrected for recent polyploidy) ranging from 2Cx = 0.3 to 65.9 pg. The raw data exhibited a strong positive relationship between outcrossing and genome size, a negative relationship between N(e) and genome size, but no detectable N(e)x outcrossing interaction. In contrast, phylogenetically independent contrast analyses found only a weak relationship between outcrossing and genome size and no relationship between N(e) and genome size. Thus, seed plants do not support the Lynch and Conery mechanism of genome size evolution. Further work is needed to disentangle contrasting effects of mating systems on the efficacy of selection and TE transmission.

Genetic diversity in Cymophyllus fraserianus (Cyperaceae), a rare monotypic genus.

Cymophyllus fraserianus (Fraser's sedge) is a rare perennial evergreen herb found in late-successional forest communities in the Appalachian Mountains of the U.S. Genetic diversity was assessed at 19 allozyme loci for 12 populations sampled from the southern and central portions of the range of this primitive monotypic genus. Species variation was comparable to that found for other narrowly distributed species, although mean population genetic diversity was somewhat higher. Sixteen of the 19 loci (84%) were polymorphic, with a mean of 46% polymorphic within populations. On average, 2.7 alleles were detected per polymorphic locus. Genetic diversity within populations (Hep) averaged 0.113, whereas species' genetic diversity (Hes) was 0.131. Although a twofold range of within-population genetic diversity (Hep = 0.075-0.158) was found, less than 13% of the genetic variation was distributed among populations (G(ST) = 0.129). An indirect estimate of historical levels of gene flow (Nm = 1.69) was consistent with the high mean genetic identity (mean I = 0.96) found between populations. Despite the relictual nature of C. fraserianus, its rarity and fragmented distribution, genetic diversity within the genus is typical of herbs with similar life-history characteristics. No association was found between geographic and genetic distances between populations (r = -0.01; p > 0.9), suggesting that present day populations are relatively isolated.

ATTERNS AND LEVELS OF POLLEN-MEDIATED GENE FLOW IN LATHYRUS LATIFOLIUS.

While gene flow can be an important force in evolution, few direct measures are available in the plant literature. Descriptions of gene movement within populations are more common, but have primarily involved crop species and artificially constructed populations. In this study, fractional paternity procedures were used to examine patterns of pollen movement over two years within two sites of Lathyrus latifolius, a bumblebee-pollinated, self-compatible perennial legume. Study sites consisted of 15 to 23 semi-discrete flowering patches that contained 1 to 29 distinct genotypes. Distributions of gene movement distances within the study sites differed significantly from that expected under random mating. Mean gene movement was 14 m. On average, 17.6% (range = 0-52%) of matings occurred within a flowering patch. Outcrossing rates, estimated from paternity analysis, ranged from 0.87 to 0.90 across sites and years. Significant heterogeneity occurred among maternal individuals with respect to outcrossing and immigration rates, indicating that mating patterns were idiosyncratic to each plant. Apparent rates of pollen flow into the sites ranged from 5 to 15%, while estimates of total pollen flow into sites ranged from 16 to 46%. Significant increases in immigration rates between years were associated with decreases in the density of flowering plants.