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1.
Genomics ; 89(1): 89-112, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16916595

RESUMO

High-resolution physically ordered gene maps for equine homologs of human chromosome 5 (HSA5), viz., horse chromosomes 14 and 21 (ECA14 and ECA21), were generated by adding 179 new loci (131 gene-specific and 48 microsatellites) to the existing maps of the two chromosomes. The loci were mapped primarily by genotyping on a 5000-rad horse x hamster radiation hybrid panel, of which 28 were mapped by fluorescence in situ hybridization. The approximately fivefold increase in the number of mapped markers on the two chromosomes improves the average resolution of the map to 1 marker/0.9 Mb. The improved resolution is vital for rapid chromosomal localization of traits of interest on these chromosomes and for facilitating candidate gene searches. The comparative gene mapping data on ECA14 and ECA21 finely align the chromosomes to sequence/gene maps of a range of evolutionarily distantly related species. It also demonstrates that compared to ECA14, the ECA21 segment corresponding to HSA5 is a more conserved region because of preserved gene order in a larger number of and more diverse species. Further, comparison of ECA14 and the distal three-quarters region of ECA21 with corresponding chromosomal segments in 50 species belonging to 11 mammalian orders provides a broad overview of the evolution of these segments in individual orders from the putative ancestral chromosomal configuration. Of particular interest is the identification and precise demarcation of equid/Perissodactyl-specific features that for the first time clearly distinguish the origins of ECA14 and ECA21 from similar-looking status in the Cetartiodactyls.


Assuntos
Mapeamento Cromossômico/veterinária , Cavalos/genética , Animais , Sequência de Bases , Evolução Biológica , Cromossomos Artificiais Bacterianos/genética , Cromossomos Humanos Par 5/genética , Cricetinae , Primers do DNA/genética , Humanos , Hibridização in Situ Fluorescente , Mamíferos/genética , Mapeamento de Híbridos Radioativos , Especificidade da Espécie
2.
Mamm Genome ; 16(8): 631-49, 2005 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16180145

RESUMO

A high-resolution (1 marker/700 kb) physically ordered radiation hybrid (RH) and comparative map of 122 loci on equine homologs of human Chromosome 19 (HSA19) shows a variant evolution of these segments in equids/Perissodactyls compared with other mammals. The segments include parts of both the long and the short arm of horse Chromosome 7 (ECA7), the proximal part of ECA21, and the entire short arm of ECA10. The map includes 93 new markers, of which 89 (64 gene-specific and 25 microsatellite) were genotyped on a 5000-rad horse x hamster RH panel, and 4 were mapped exclusively by FISH. The orientation and alignment of the map was strengthened by 21 new FISH localizations, of which 15 represent genes. The approximately sevenfold-improved map resolution attained in this study will prove extremely useful for candidate gene discovery in the targeted equine chromosomal regions. The highlight of the comparative map is the fine definition of homology between the four equine chromosomal segments and corresponding HSA19 regions specified by physical coordinates (bp) in the human genome sequence. Of particular interest are the regions on ECA7 and ECA21 that correspond to the short arm of HSA19-a genomic rearrangement discovered to date only in equids/Perissodactyls as evidenced through comparative Zoo-FISH analysis of the evolution of ancestral HSA19 segments in eight mammalian orders involving about 50 species.


Assuntos
Cromossomos Humanos Par 19/genética , Evolução Molecular , Cavalos/genética , Mamíferos/genética , Mapeamento Físico do Cromossomo , Animais , Cromossomos Artificiais Bacterianos , Marcadores Genéticos , Genoma , Humanos , Hibridização in Situ Fluorescente , Metáfase , Repetições de Microssatélites , Mapeamento de Híbridos Radioativos
3.
Genomics ; 85(2): 188-200, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15676277

RESUMO

High-resolution gene maps of individual equine chromosomes are essential to identify genes governing traits of economic importance in the horse. In pursuit of this goal we herein report the generation of a dense map of horse chromosome 22 (ECA22) comprising 83 markers, of which 52 represent specific genes and 31 are microsatellites. The map spans 831 cR over an estimated 64 Mb of physical length of the chromosome, thus providing markers at approximately 770 kb or 10 cR intervals. Overall, the resolution of the map is to date the densest in the horse and is the highest for any of the domesticated animal species for which annotated sequence data are not yet available. Comparative analysis showed that ECA22 shares remarkable conservation of gene order along the entire length of dog chromosome 24, something not yet found for an autosome in evolutionarily diverged species. Comparison with human, mouse, and rat homologues shows that ECA22 can be traced as two conserved linkage blocks, each related to individual arms of the human homologue-HSA20. Extending the comparison to the chicken genome showed that one of the ECA22 blocks that corresponds to HSA20q shares synteny conservation with chicken chromosome 20, suggesting the segment to be ancestral in mammals and birds.


Assuntos
Cromossomos , Cavalos/genética , Mapeamento de Híbridos Radioativos/métodos , Vertebrados/genética , Animais , Evolução Biológica , Cromossomos Artificiais Bacterianos , Ordem dos Genes , Humanos , Hibridização in Situ Fluorescente , Camundongos , Reação em Cadeia da Polimerase/métodos , Ratos , Sitios de Sequências Rotuladas
4.
Genome Res ; 13(4): 742-51, 2003 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12671008

RESUMO

A first-generation radiation hybrid (RH) map of the equine (Equus caballus) genome was assembled using 92 horse x hamster hybrid cell lines and 730 equine markers. The map is the first comprehensive framework map of the horse that (1) incorporates type I as well as type II markers, (2) integrates synteny, cytogenetic, and meiotic maps into a consensus map, and (3) provides the most detailed genome-wide information to date on the organization and comparative status of the equine genome. The 730 loci (258 type I and 472 type II) included in the final map are clustered in 101 RH groups distributed over all equine autosomes and the X chromosome. The overall marker retention frequency in the panel is approximately 21%, and the possibility of adding any new marker to the map is approximately 90%. On average, the mapped markers are distributed every 19 cR (4 Mb) of the equine genome--a significant improvement in resolution over previous maps. With 69 new FISH assignments, a total of 253 cytogenetically mapped loci physically anchor the RH map to various chromosomal segments. Synteny assignments of 39 gene loci complemented the RH mapping of 27 genes. The results added 12 new loci to the horse gene map. Lastly, comparison of the assembly of 447 equine genes (256 linearly ordered RH-mapped and additional 191 FISH-mapped) with the location of draft sequences of their human and mouse orthologs provides the most extensive horse-human and horse-mouse comparative map to date. We expect that the foundation established through this map will significantly facilitate rapid targeted expansion of the horse gene map and consequently, mapping and positional cloning of genes governing traits significant to the equine industry.


Assuntos
Sequência Conservada/genética , Genoma Humano , Genoma , Cavalos/genética , Mapeamento de Híbridos Radioativos/métodos , Mapeamento de Híbridos Radioativos/veterinária , Animais , Linhagem Celular , Cricetinae , Marcadores Genéticos/genética , Marcadores Genéticos/efeitos da radiação , Humanos , Células Híbridas , Hibridização in Situ Fluorescente/métodos , Hibridização in Situ Fluorescente/estatística & dados numéricos , Hibridização in Situ Fluorescente/veterinária , Camundongos , Repetições de Microssatélites/genética , Repetições de Microssatélites/efeitos da radiação , Dados de Sequência Molecular , Mapeamento de Híbridos Radioativos/estatística & dados numéricos , Alinhamento de Sequência/métodos , Alinhamento de Sequência/estatística & dados numéricos , Alinhamento de Sequência/veterinária , Distribuições Estatísticas , Sintenia/genética , Sintenia/efeitos da radiação
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