[Progesterone - regulator of T-lymphocytes cytotoxicity].

Progesterone plays important the role in the regulation of the immune system during pregnancy. We examined the effect of progesterone on the cytotoxic activity of T-lymphocytes in a model system Jurkat cells. Jurkat cells were stimulated with 50 µg/ml of phytohemaglutinin A (PHA) at 370C for 5 minutes. Then, PHA was removed by centrifugation, the cells were washed and cultured for 24 hours alone or with progesterone (added to the incubation medium of Jurkat cells at a concentration of 0.07 and 0.7 µl. Determined value of The parameters of mitochondrial membrane potential (by flow cytometry) and parameters of mitochondrial oxidative metabolism (the rate of generation of superoxide and peroxide radicals and activity of mitochondrial superoxide dismutase, catalase, glutatinperoxidase, the degree of nitrosylation of mitochondrial electron-transport proteins (by method of electron paramagnetic resonance and spectrophotometry) were determined. It was identified the lowering effect of high dose of progesterone (0.7µl) on the value of mitochondrial membrane potential (balancing percentage of cells with high and low values of mitochondrial potential and decreased intensity of oxidative stress in mitogen - activated Jurkat cells, which supports inhibition of their proliferation and differentiation activity.

[The role of neuroendocrine mediators in regulatory activity of T-cells].

The aim of the study was to establish the role of some neuroendocrine mediators (agonists and antagonists of ß-adrenergic receptors, progesterone) in regulating T-cells activity. Studies conducted on the culture of leukemiatransformed T-cells (Jurkat cells) (DSMZ-Deutshe Sammulung von Mikroorganismen und Zellkulturen (Germany)). Jurkat Cells (4 x 105 cells/ml) stimulated with 50 µg/ml fitogemaglutinin A (PHA) at 370С for 5 minutes and then incubated for 24 hours alone, or together with ß- adrenergic receptors agonist izopretonolom (at dose of 10-5 M, 10-6 M), an antagonist, propranolol (dose of 10-5 M, 10-6 M) and progesterone (dose 0,07 µl, 0,7 µl) added to the incubation medium. The viability of Jurkat cells was determined by MTT test. It was shown that ß-adlenoretseptors agonist, izoprotenol didn't affect the activity of mitochondrial dehydrogenases in intact and contributed to their low activation (12%) in the mitogen-activated Jurkat cells. ß-adrenergic receptors antagonist, propranolol, promotes a significant reduction in activity of mitochondrial dehydrogenases, and hence the viability of both intact (40-60%) and mitogen stimulated Jurkat cells (20-40%). Viability of intact Jurkat cells didn't change, and dose-dependently increased in PHA-stimulated Jurkat cells during progesterone exposure. It was concluded that viability of the T-cells and hence their functional activity is largely sensitive to the influence of the ß-adrenoceptor antagonists and progesterone. These data should be considered in the clinical application of appropriate drugs.