RESUMO
In our previous investigation Insulin Receptor Substrate 1 (IRS-1) correlated with proliferation marker Ki-67 in human breast cancer. The aim of the present study was to assess relationships between IRS-1 expression and anti-apoptotic Bcl-xL as well as proapoptotic Bax proteins, assessed by immunohistochemistry, in primary tumors and lymph node metastases of breast cancer. IRS-1 is positively associated with both Bcl-xL and Bax in primary and metastatic tumors. Thus, our results could suggest that IRS-1 might affect turnover of cancer cells and breast cancer progression through activation of mitogenesis and participation in the regulation of the balance between anti- and proapoptotic pathways.
Assuntos
Biomarcadores Tumorais/análise , Neoplasias da Mama/metabolismo , Metástase Linfática/patologia , Fosfoproteínas/biossíntese , Proteína X Associada a bcl-2/biossíntese , Proteína bcl-X/biossíntese , Neoplasias da Mama/patologia , Feminino , Humanos , Imuno-Histoquímica , Proteínas Substratos do Receptor de Insulina , Pessoa de Meia-IdadeRESUMO
Activity of lysosomal (cathepsins A,B,C,D and E) and nonlysosomal proteases (cathepsin G, elastase, collagenase, prolidase, prolinase) was evaluated in fibrosarcoma induced in rats by methylcholanthrene. No differences were found in the activity of the examined proteases in tumours of different size in the external, intermediate and central spheres of these tumours. Activity of cathepsins A,B,C,D,E and G, prolidase and prolinase was higher in the fibrosarcoma and activity of collagenase and elastase was lower than in the rat skin.
Assuntos
Carcinógenos , Endopeptidases/metabolismo , Fibrossarcoma/enzimologia , Lisossomos/enzimologia , Metilcolantreno , Neoplasias Cutâneas/enzimologia , Animais , Carboxipeptidases/metabolismo , Catepsina A , Catepsina B/metabolismo , Catepsina C , Catepsina D/metabolismo , Catepsina E , Catepsina G , Catepsinas/metabolismo , Colagenases/metabolismo , Dipeptidases/metabolismo , Dipeptidil Peptidases e Tripeptidil Peptidases/metabolismo , Fibrossarcoma/induzido quimicamente , Fibrossarcoma/patologia , Masculino , Ratos , Ratos Wistar , Serina Endopeptidases/metabolismo , Pele/enzimologia , Neoplasias Cutâneas/induzido quimicamente , Neoplasias Cutâneas/patologiaRESUMO
Activity of lysosomal and nonlysosomal proteases and contents of protein and its degradation products in the blood serum of rats with methylcholantrene fibrosarcoma were evaluated. Activity of lysosomal proteases and prolidase and prolinase as well in the blood serum of rats with methylcholanthrene tumour did not differ from the activity of these enzymes in the blood serum of control rats. Only the activity of elastase and collagenase in the blood serum of rats with methylcholanthrene tumour especially with tumour of intermediate and big mass was increased. Content of total protein was decreased in the blood serum of rats with tumour of intermediate and big mass and contents of glycoproteins and alfa-amin nitrogen were increased in comparison to the blood serum of control rats.
