RESUMO
Metalloproteases, plasminogen urokinase activator, plasmin and cathepsins enable the expansion of neoplastic tumors, leading to metastases formation. They cause neoplastic cells to detach from tumor, facilitate cell movement, implantation and participate in tumor vascularization. The regulation of these processes is accomplished during the synthesis and activation of proenzymes. Enzyme activity control is realized by their bonds with cellular membranes, and inhibitor action.
Assuntos
Divisão Celular/fisiologia , Endopeptidases/fisiologia , Metástase Neoplásica/fisiopatologia , Proteínas de Neoplasias/fisiologia , Animais , Catepsinas/fisiologia , Fibrinolisina/fisiologia , Humanos , Lisossomos/enzimologia , Metaloendopeptidases/fisiologia , Invasividade Neoplásica , Ativador de Plasminogênio Tipo Uroquinase/fisiologiaRESUMO
Activity of lysosomal (cathepsins A,B,C,D and E) and nonlysosomal proteases (cathepsin G, elastase, collagenase, prolidase, prolinase) was evaluated in fibrosarcoma induced in rats by methylcholanthrene. No differences were found in the activity of the examined proteases in tumours of different size in the external, intermediate and central spheres of these tumours. Activity of cathepsins A,B,C,D,E and G, prolidase and prolinase was higher in the fibrosarcoma and activity of collagenase and elastase was lower than in the rat skin.
Assuntos
Carcinógenos , Endopeptidases/metabolismo , Fibrossarcoma/enzimologia , Lisossomos/enzimologia , Metilcolantreno , Neoplasias Cutâneas/enzimologia , Animais , Carboxipeptidases/metabolismo , Catepsina A , Catepsina B/metabolismo , Catepsina C , Catepsina D/metabolismo , Catepsina E , Catepsina G , Catepsinas/metabolismo , Colagenases/metabolismo , Dipeptidases/metabolismo , Dipeptidil Peptidases e Tripeptidil Peptidases/metabolismo , Fibrossarcoma/induzido quimicamente , Fibrossarcoma/patologia , Masculino , Ratos , Ratos Wistar , Serina Endopeptidases/metabolismo , Pele/enzimologia , Neoplasias Cutâneas/induzido quimicamente , Neoplasias Cutâneas/patologiaRESUMO
Activity of lysosomal and nonlysosomal proteases and contents of protein and its degradation products in the blood serum of rats with methylcholantrene fibrosarcoma were evaluated. Activity of lysosomal proteases and prolidase and prolinase as well in the blood serum of rats with methylcholanthrene tumour did not differ from the activity of these enzymes in the blood serum of control rats. Only the activity of elastase and collagenase in the blood serum of rats with methylcholanthrene tumour especially with tumour of intermediate and big mass was increased. Content of total protein was decreased in the blood serum of rats with tumour of intermediate and big mass and contents of glycoproteins and alfa-amin nitrogen were increased in comparison to the blood serum of control rats.
