Seedling survival after simulating grazing and drought for two species from the Pamirs, northwestern China.

For plant populations to persist, seedling recruitment is essential, requiring seed germination, seedling survival and growth. Drought and grazing potentially reduce seedling recruitment via increased mortality and reduced growth. We studied these seed-related processes for two species indigenous to the Pamir Mountains of Xinjiang in northwestern China: Saussurea glacialis and Plantago lessingii. Seeds collected from Taxkorgan, Xinjiang, had a viability rate of 15.8% for S. glacialis but 100% for P. lessingii. Of the viable seeds, the highest germination rates were 62.9% for S. glacialis and 45.6% for P. lessingii. In a greenhouse experiment, we imposed a series of stressful conditions, involving a combination of simulated grazing and drought events. These had the most severe impact on younger seedlings. Modelling showed that 89% of S. glacialis mortality was due to early simulated grazing, whereas 80% of P. lessingii mortality was due to early simulated drought. Physiological differences could contribute to their differing resilience. S. glacialis may rely on water storage in leaves to survive drought events, but showed no shifts in biomass allocation that would improve grazing tolerance. P. lessingii appears more reliant on its root system to survive grazing, but the root reserves of younger plants could be insufficient to grow deeper in response to drought. After applying all mortality factors, 17.7 seedlings/parent of P. lessingii survived, while only <0.1 seedlings/parent of S. glacialis survived, raising concerns for its capacity to persist in the Pamirs. Inherent genetic differences may underlie the two species' contrasting grazing and drought responses. Thus, differing conservation strategies are required for their utilization and protection.

Phosphorus mitigation remains critical in water protection: A review and meta-analysis from one of China's most eutrophicated lakes.

The processes of urbanization and industrialization within geological phosphorus-rich mountains (GPMn) have resulted in water degradation within southwest China. Lake Dianchi, one of the most eutrophicated lakes in China, has epitomized this issue. Clear understandings of phosphorus (P) mitigation efforts, the evolution of P budgets, and possible risks in the Dianchi system will benefit future eutrophication control, providing valuable lessons for other plateau freshwater lakes. In this study, we applied systematic review methodology to investigate the above questions, and then compared the results with other lakes worldwide. Generally, meta-analytical approaches have indicated P levels remain a key factor in causing algal blooms. Post-2015, the P budget of the Dianchi system, especially in Caohai section, was modified. However, it's still experiencing high pressures from P enrichment (Caohai: 0.4 mg·l-1; Waihai: 0.2 mg·l-1). The flux of P in Dianchi remains high, both through the external P load (556 ton·a-1), and an internal cycle (304 ton·a-1 associated with the absorption, deposition and removal of algae biomass; and 380 ton·a-1 associated with sediment exchange). Meanwhile, significant P retention has been observed in the lake, in particular within the Waihai section (211 ton·a-1). Currently, water diversion (from external watersheds), sewage diversion, and sediment-dredging projects have benefited Dianchi. However, continuous urbanization and GPMn ecological degradation could introduce hundreds of tons of additional P, leading to subsequent algal blooms. Furthermore, beyond Lake Dianchi, other lakes and reservoirs in southwest China are facing similar issues regarding P mitigation, especially in GPMn regions, though corresponding knowledge is still limited. Therefore, effective and flexible sub-regional protection strategies and research related to external and internal P mitigations have become key requirements for Lake Dianchi management. Meanwhile, ecologically sensitive approaches to GPMn regions, as well as city development within basin and market driven treatments, should be incorporated into regional water source protection for southwest China.

Assessment of Indirect N2O Emission Factors from Agricultural River Networks Based on Long-Term Study at High Temporal Resolution.

Assessment of indirect emission factors (EF5r) of nitrous oxide (N2O) from agricultural river networks remains challenging, and results are uncertain due to limited data availability. This study compared two methods of assessing EF5r using data from long-term observations at high temporal resolution in a typical agricultural catchment in subtropical central China. The concentration method (method 1) and the Intergovernmental Panel on Climate Change (IPCC) 2006 method (method 2) were employed to evaluate the emission factor. EF5r estimated using method 1 (i.e., EF5r1) was 0.00077 ± 0.00025 (0.00038-0.00097). EF5r calculated using method 2 (i.e., EF5r2) was lower than EF5r1, with a mean value of 0.00004 (0.000015-0.00012). Both EF5r1 and EF5r2 were significantly lower than the IPCC 2006 default value of 0.0025, suggesting that N2O emissions from China and world river networks may be grossly overestimated. A complex N2O production pathway and diffusion mechanism were responsible for the transfer of N2O from the sediment to river water and then to the atmosphere. These findings provide essential data for refining national greenhouse gas inventories and contribute evidence for downward revision of indirect emission factors adopted by the IPCC.

Collaborative use of the peer assist model in large transplant programs in the United States.

CONTEXT: The basic assumption of the peer assist model is that nearly every transplant center is already doing something that other centers could adopt in order to streamline or enhance their own operations. OBJECTIVE: To describe how the Transplant Growth and Management Collaborative used the peer assist model with 2 large transplant centers in the United States and to identify best practices and outcomes. SETTING: The University of California, San Francisco, Medical Center. PARTICIPANTS: Transplant health care providers (surgeons, nurse transplant coordinators) and administrative staff (program directors, financial advisers, quality representatives). INTERVENTION: The peer assist model is a mechanism by which a transplant center receives feedback about a specific topic from another transplant center that encountered similar issues and developed effective action plans for overcoming barriers to success; it is a reciprocal model in which knowledge is shared. RESULTS: The peer assist model benefited the preparation for accelerated growth in 2 of the largest transplant centers in the United States. The collaboration during peer assist visits is an opportune time for transplant centers to learn from one another.

N(2)O concentration and isotope signature along profiles provide deeper insight into the fate of N(2)O in soils.

Nitrous oxide is an important greenhouse gas and its origin and fate are thus of broad interest. Most studies on emissions of nitrous oxide from soils focused on fluxes between soil and atmosphere and hence represent an integration of physical and biological processes at different depths of a soil profile. Analysis of N(2)O concentration and isotope signature along soil profiles was suggested to improve the localisation of sources and sinks in soils as well as underlying processes and could therefore extend our knowledge on processes affecting surface N(2)O fluxes. Such a mechanistic understanding would be desirable to improve N(2)O mitigation strategies and global N(2)O budgets. To investigate N(2)O dynamics within soil profiles of two contrasting (semi)natural ecosystem types (a temperate acidic fen and a Norway spruce forest), soil gas samplers were constructed to meet the different requirements of a water-saturated and an unsaturated soil, respectively. The samplers were installed in three replicates and allowed soil gas sampling from six different soil depths. We analysed soil air for N(2)O concentration and isotope composition and calculated N(2)O net turnover using a mass balance approach and considering diffusive fluxes. At the fen site, N(2)O was mainly produced in 30-50 cm soil depth. Diffusion to adjacent layers above and below indicated N(2)O consumption. Values of delta(15)N and delta(18)O of N(2)O in the fen soil were always linearly correlated and their qualitative changes within the profile corresponded with the calculated turnover processes, suggesting further reduction of N(2)O. In the spruce forest, highest N(2)O production occurred in the topsoil, but there was also notable production occurring in the subsoil at a depth of 70 cm. Changes in N(2)O isotope composition as to be expected from local production and consumption processes within the soil profile did hardly occur, though. This was presumably caused by high diffusive fluxes and comparatively low net turnover, as isotope signatures approached values measured for ambient N(2)O towards the topsoil. Our results demonstrate a highly variable influence of diffusive versus production/consumption processes on N(2)O concentration and isotope composition, depending on the type of ecosystem. This finding indicates the necessity of further N(2)O concentration and isotope profile investigations in different types of natural and anthropogenic ecosystems in order to generalise our mechanistic understanding of N(2)O exchange between soil and atmosphere.