Assuntos
Ativação Linfocitária , Sangue , Carcinógenos/farmacologia , Divisão Celular , Linhagem Celular , Transformação Celular Neoplásica , Células Cultivadas , Feminino , Herpesvirus Humano 4 , Humanos , Hidrocortisona/farmacologia , Mononucleose Infecciosa , Lectinas , Ativação Linfocitária/efeitos dos fármacos , Linfócitos/imunologia , Masculino , Mutagênicos/farmacologia , Vírus Rauscher , Vírus 40 dos Símios , Cordão UmbilicalRESUMO
The relative capacity of several types of human cells and tissue to produce interferon was studied. Types of cells and tissue included were fibroblasts from embryos, foreskins, and biopsied skins; amnion cells; peripheral leukocytes; established lymphoid cell lines; established heteroploid cell lines; and chorioamniotic membrane. When Newcastle disease virus was used as the inducer, fibroblasts and amnion cells produced more interferon per 10(6) cells than leukocytes, lymphoid cells, and heteroploid cells. Only minor variations in interferon-producing capacity were observed among fibroblasts from 36 persons. Culture passage level, cell concentration, and inducer were factors that significantly affected interferon production.
Assuntos
Células Cultivadas/metabolismo , Interferons/biossíntese , Adulto , Âmnio , Linhagem Celular , Vírus Chikungunya , Criança , Técnicas de Cultura , Embrião de Mamíferos , Feminino , Fibroblastos , Células HeLa , Humanos , Mononucleose Infecciosa/sangue , Leucemia Mieloide/sangue , Leucócitos , Tecido Linfoide , Masculino , Vírus da Doença de Newcastle , Pênis , Pele , Fatores de Tempo , Vírus da Estomatite Vesicular IndianaRESUMO
Nineteen lines of human fibroblasts were inoculated with a filtrate prepared from the ST feline sarcoma. Seven lines showed morphological alteration and released focus-forming activity for feline cells, 2 lines showed morphological alteration but did not release focus-forming activity, and 11 lines showed no morphological alteration and released no focus-forming agent. Morphologically altered cells appeared enlarged, hyper-refractile, and intensely stained by hematoxylin. They neither assumed a crisscross pattern nor piled up to form a visible focus. Time-lapse cinegraph showed that the morphologically altered cells did not divide and were motile. The fluid from two human fibroblast cultures, inoculated 4 and 14 weeks previously with the ST sarcoma filtrate, induced fibrosarcoma in newborn kittens.
