Assuntos
Trifosfato de Adenosina/análogos & derivados , Núcleo Celular/enzimologia , RNA Polimerases Dirigidas por DNA/antagonistas & inibidores , Desoxiadenosinas/farmacologia , Nucleotidiltransferases/antagonistas & inibidores , Polinucleotídeo Adenililtransferase/antagonistas & inibidores , Saccharomyces cerevisiae/enzimologia , Trifosfato de Adenosina/farmacologia , RNA Polimerases Dirigidas por DNA/isolamento & purificação , Cinética , Mutação , Polinucleotídeo Adenililtransferase/isolamento & purificaçãoAssuntos
Genes , Mutação , Saccharomyces cerevisiae/metabolismo , Cruzamentos Genéticos , Cicloeximida/farmacologia , DNA/biossíntese , DNA Mitocondrial/biossíntese , Diploide , Resistência Microbiana a Medicamentos , Eritromicina/farmacologia , Genótipo , Mitocôndrias/metabolismo , Oligomicinas/farmacologia , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/isolamento & purificaçãoRESUMO
The effect of the antibiotic nalidixic acid on macromolecular metabolism in the yeast Saccharomyces cerevisiae has been studied. It was found that, upon the addition of nalidixic acid to a logarithmically growing culture, there is a transient inhibition of total cell ribonucleic acid, deoxyribonucleic acid, and protein synthesis, after which the cells show an almost complete recovery. In addition, there is no preferential inhibition of yeast mitochondrial deoxyribonucleic acid synthesis.
Assuntos
Ácido Nalidíxico/farmacologia , Saccharomyces cerevisiae/efeitos dos fármacos , Centrifugação com Gradiente de Concentração , DNA/biossíntese , Proteínas Fúngicas/biossíntese , RNA/biossíntese , Saccharomyces cerevisiae/metabolismo , EsferoplastosRESUMO
Two kinds of phenotypic expression in purine biosynthesis result from recessive mutation to adel2 in baker's yeast. The mutants are adenine-specific, blocked in the conversion of inosine 5'-phosphate to adenylosuccinic acid; their response to inhibition of pathway activity by adenine is considerably reduced. Allelic partial reversions can restore prototrophy without correcting the regulatory defect imparted by the primary mutation. The separation of the two properties of the locus by allelic mutation supports the hypothesis that the locus specifies a protein of two independent functions, enzymatic and regulatory.
