Assuntos
Plasma , Sorotipagem , Citratos/efeitos adversos , Humanos , Resinas Vegetais/efeitos adversosRESUMO
A single-channel AutoAnalyzer was used to screen potential blood-grouping sera to exclude the presence of unwanted antibodies. In order to detect the widest possible range of antibodies, a low-ionic strength Polybrene method was employed, but difficulty was encountered because the aggregated cells adhered to the glass surfaces of the reaction coils, resulting in instability of the system. Modifications were made to the apparatus, and the improvements in the demonstration of antibodies that resulted are described.
Assuntos
Autoanálise/métodos , Eritrócitos/imunologia , Isoanticorpos/análise , Sistema ABO de Grupos Sanguíneos , Anticorpos , Brometo de Hexadimetrina/farmacologia , Humanos , Soros Imunes , PolietilenoglicóisAssuntos
Anemia Hemolítica Autoimune , Antígenos de Grupos Sanguíneos , Isoantígenos/análise , Adolescente , Agranulocitose/induzido quimicamente , Azatioprina/efeitos adversos , Transfusão de Sangue , Sobrevivência Celular , Isótopos do Cromo , Teste de Coombs , Eritrócitos , Hemoglobinometria , Humanos , Isoanticorpos , Masculino , Trombocitopenia/induzido quimicamenteAssuntos
Antígenos de Grupos Sanguíneos , Isoantígenos/análise , Animais , Especificidade de Anticorpos , Antígenos , Feminino , Testes de Inibição da Hemaglutinação , Humanos , Soros Imunes , Imunogenética , Imunoglobulina G , Isoantígenos/classificação , Mutação , Proteínas do Mieloma , Pan troglodytes , Coelhos , Especificidade da EspécieAssuntos
Sistema do Grupo Sanguíneo Rh-Hr , gama-Globulinas/uso terapêutico , alfa-Globulinas/análise , Anticorpos/análise , Antígenos , Antígenos de Grupos Sanguíneos , Eletroforese das Proteínas Sanguíneas , Cromatografia , Dextranos , Armazenamento de Medicamentos , Feminino , Humanos , Soros Imunes/análise , Imunodifusão , Imunoglobulinas/uso terapêutico , Macroglobulinas/análise , Masculino , Albumina Sérica/análise , Transferrina/análise , UltracentrifugaçãoRESUMO
Some time ago the WHO Expert Committee on Biological Standardization asked the National Institute for Medical Research, London, to consult with the WHO International Blood Group Reference Laboratory concerning the establishment of an international standard for albumin-potentiated anti-Rh(o) (anti-D) blood-typing serum. This paper describes the collection and testing of sera, the removal of unwanted antibodies, the pooling of the sera and the distribution in equal amounts in 3350 ampoules, which were subsequently freeze-dried. The titres were determined by 29 laboratories in 21 countries by means of four methods-suspension in albumin, replacement with albumin, enzyme (papain) treatment and the anti-human-globulin test. On the basis of the results obtained, the material has been established as the International Standard for Anti-Rh(o) (Anti-D) Incomplete Blood-Typing Serum and the International Unit has been defined, with the agreement of the participants in the collaborative study, as the activity contained in 0.95 mg of the International Standard for Anti-Rh(o) (Anti-D) Incomplete Blood-Typing Serum.
