RESUMO
Burkholderia pseudomallei was quickly identified from blood cultures collected from septicemic patients by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis using an in-house reference library. This procedure reduced the time to definitive identification by more than 24 hours. This analysis is a useful addition to laboratory methods for early recognition of septicemic melioidosis in non-endemic settings.
Assuntos
Burkholderia pseudomallei/isolamento & purificação , Melioidose/diagnóstico , Melioidose/microbiologia , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Antibacterianos/uso terapêutico , Austrália , Burkholderia pseudomallei/efeitos dos fármacos , Burkholderia pseudomallei/crescimento & desenvolvimento , Burkholderia pseudomallei/patogenicidade , Humanos , Masculino , Melioidose/tratamento farmacológico , Meropeném , Pessoa de Meia-Idade , Tienamicinas/uso terapêuticoRESUMO
We report the first isolation in Australia of a hypervirulent epidemic strain of Clostridium difficile, PCR ribotype 027. It was isolated from a 43-year-old woman with a permanent ileostomy, who appears to have been infected while travelling in the United States. The isolate was positive for toxin A, toxin B and binary toxin, and resistant to fluoroquinolone antimicrobials, and had characteristic deletions in the tcdC gene. All diagnostic laboratories and health care facilities in Australia should now be on high alert for this organism.
Assuntos
Clostridioides difficile/isolamento & purificação , DNA Bacteriano/análise , Enterocolite Pseudomembranosa/microbiologia , Reação em Cadeia da Polimerase/métodos , Ribotipagem/métodos , Adulto , Austrália/epidemiologia , Clostridioides difficile/genética , Diagnóstico Diferencial , Enterocolite Pseudomembranosa/diagnóstico , Enterocolite Pseudomembranosa/etnologia , Feminino , Humanos , Viagem , Estados Unidos/etnologiaRESUMO
BACKGROUND AND OBJECTIVE: To investigate the association between serological evidence of past infections with common respiratory pathogens and lung function in members of an isolated community of Aborigines from tropical coastal north-western Australia. METHODS: FEV(1) and FVC were assessed by dry bellows spirometer. Serum IgG titres to 11 common respiratory pathogens were assayed. Smoking history was assessed by questionnaire. Reciprocal positive IgG titres were taken as >or=10 for all pathogens with the exception of Legionella spp. (>or=40) and Burkholderia pseudomallei (>or=20). Linear regression analysis examined associations between titres and lung function after adjustment for age, height, gender and smoking, separately for adults (age > 17 years) and children. RESULTS: An increased total number of positive IgG titres was significantly associated with reduced FEV(1) (P = 0.01) and FEV(1)/FVC ratio (P = 0.01) suggesting the presence of airflow obstruction. This association was independent of age, gender, height, weight and smoking status. CONCLUSIONS: The burden of past respiratory infections may be an important determinant of airway function in this Aboriginal community.
