Effectiveness of UV-C radiation in inactivation of microorganisms on materials with different surface structures.

INTRODUCTION AND OBJECTIVE: Ultraviolet light in the UV-C band is known as germicidal radiation and was widely used for both sterilization of the equipment and creation of a sterile environment. The aim of the study is to assess the effectiveness of inactivation of microorganisms deposited on surfaces with various textures by UV-C radiation disinfection devices. MATERIAL AND METHODS: Five microorganisms (3 bacteria, virus, and fungus) deposited on metal, plastic, and glass surfaces with smooth and rough textures were irradiated with UV-C light emitted by low-pressure mercury lamp and ultraviolet emitting diodes (LEDs), from a distance of 0.5 m, 1 m, and 1.5 m to check their survivability after 20-minute exposure. RESULTS AND CONCLUSIONS: Both tested UV-C sources were effective in inactivation of microorganisms; however, LED emitter was more efficient in this respect than the mercury lamp. The survival rate of microorganisms depended on the UV-C dose, conditioned by the distance from UV-C source being the highest at 0.5 m and the lowest at 1.5 m. For the tested microorganisms, the highest survival rate after UV-C irradiation was usually visible on glass and plastic surfaces. This observation should be considered in all environments where the type of material (from which the elements of technical equipment are manufactured and may be contaminated by specific activities) is important for maintaining the proper level of hygiene and avoiding the unwanted and uncontrolled spread of microbiological pollution.

Monitoring of enteropathogenic Gram-negative bacteria in wastewater treatment plants: a multimethod approach.

The wastewater treatment processes are associated with the emission of microbial aerosols, including enteropathogenic bacteria. Their presence in this work environment poses a real threat to the health of employees, both through the possibility of direct inhalation of the contaminated air and indirectly through the pollution of all types of surfaces with such bioaerosol particles. This study aimed to investigate the prevalence of enteropathogenic bacteria in the air, on surfaces, and in wastewater samples collected in four wastewater treatment plants (WWTPs). The effectiveness of conventional culture-biochemical, as well as spectrometric and molecular methods for the rapid detection of enteropathogenic bacteria at workstations related to particular stages of wastewater processing, was also evaluated. Bioaerosol, surface swab, and influent and effluent samples were collected from wastewater plants employing mechanical-biological treatment technologies. The air samples were collected using MAS-100 NT impactor placed at a height of 1.5 m above the floor or ground, simulating aspiration from the human breathing zone. Surface samples were collected with sterile swabs from different surfaces (valves, handles, handrails, and coveyor belts) at workplaces. The raw influent and treated effluent wastewater samples were aseptically collected using sterile bottles. The identification of bacterial entheropathogens was simultaneously conducted using a culture-based method supplemented with biochemical (API) tests, mass-spectrometry (MALDI TOF MS), and molecular (multiplex real-time PCR) methods. This study confirmed the common presence of bacterial pathogens (including enteropathogenic and enterotoxigenic Escherichia coli, Salmonella spp., Campylobacter spp., and Yersinia enterocolitica) in all air, surface, and wastewater samples at studied workplaces. Higher concentrations of enteropathogenic bacteria were observed in the air and on surfaces at workplaces where treatment processes were not hermetized. The results of this study underline that identification of enteropathogenic bacteria in WWTPs is of great importance for the correct risk assessment at workplaces. From the analytical point of view, the control of enteropathogenic bacterial air and surface pollution using rapid multiplex-PCR method should be routinely performed as a part of hygienic quality assessment in WWTPs.

Production of pro-inflammatory mediators stimulated by exposure of poultry house workers to airborne dust particulates.

INTRODUCTION AND OBJECTIVE: Poultry house employees spend a significant part of their work shift being exposed to airborne particulate pollutants. The aim of this study was to assess their exposure at different stages of chicken production cycle, based on quantification of pro-inflammatory mediators (IL-1ß, IL-6, IL-8, and TNFα) in nasal lavage (NAL) samples. MATERIAL AND METHODS: The concentrations of airborne dust at 3 different stages of the production cycle (i.e. empty poultry house, with 7- and 42-day-old chickens) were stationary measured using Grimm spectrometer, as well as CIS and Button samplers. The dust collected by the latter 2 samplers was analyzed for endotoxin and (1→3)-ß-D-glucan content. NAL samples were collected from employees after their work shift to determine the pro-inflammatory mediator levels. RESULTS: The maximum particulate aerosol, endotoxin, and (1→3)-ß-D-glucan concentrations at workplaces reached the levels of 4.12 mg/m3, 45.21 ng/m3, and 56.54 ng/m3, respectively. The IL-1ß, IL-6, and IL-8 concentrations in NAL samples ranged between 0.62-18.12 pg/mL, <0.70-25.37 pg/mL, and <3.50-259.5 pg/mL, respectively. All TNFα levels were below 4 pg/mL. There were no significant differences between these cytokine concentrations in NAL samples collected at different stages of chicken breeding in either 'winter' or 'summer' seasons. CONCLUSIONS: Inhalation stimulation with poultry dust containing endotoxins and (1→3)-ß-D-glucans resulted in the production of pro-inflammatory mediators, which proves the course of immunological processes in the exposed employees that may lead to adverse effects. The use of nasal lavage fluid in the control of such exposure confirms that NAL analysis is a reliable laboratory tool for assessing the impact of poultry dust on exposed farm workers.

Poultry house as point source of intense bioaerosol emission.

INTRODUCTION AND OBJECTIVE: Intensive poultry farming is usually associated with massive exposure to organic dust, which is largely composed of microbiological origin particulates. The aim of the study is to assess occupational and environmental exposures to airborne bacteria, fungi, and Marek's disease virus emitted by a poultry house. MATERIAL AND METHODS: The concentrations of airborne microorganisms in a poultry house and its vicinity (250-500 m) at 3 different stages of the production cycle (i.e. empty poultry house, with 7-day-old and 42-day-old chickens) were stationary measured using Andersen and MAS impactors, as well as Coriolis and BioSampler impingers. The collected microbiota was taxonomically identified using molecular and biochemical techniques to characterize occupational exposure and its spatial dissemination. RESULTS: Although Marek's disease virus was not present in the tested air samples, the appearance of reared chickens in the poultry house resulted in an increase in airborne bacterial and fungal concentrations up to levels of 1.26 × 108 CFU/m3 and 3.77 × 104 CFU/m3, respectively. These pollutants spread around through the ventilation system, but their concentrations significantly decreased at a distance of 500 m from the chicken coop. A part of the identified microbiota was pathogens that were successfully isolated from the air by all 4 tested samplers. CONCLUSIONS: The poultry house employees were exposed to high concentrations of airborne microorganisms, including pathogens that may lead to adverse health outcomes. To protect them, highly efficient hygienic and technical measures regarding the poultry house interior and its ventilation, respectively, should be introduced to prevent both unwanted pollution and subsequent emission of microbial contaminants during intensive chicken breeding.

Filters of automobile air conditioning systems as in-car source of exposure to infections and toxic moulds.

The main component of an air conditioning system is air filters. Over time, the filters of an air conditioning system in cars can turn into sources of emission of microbiological hazards. The aim of this study was to quantitatively and qualitatively assess the presence of infectious and toxic fungi in the AC filters in passenger cars. The studied non-woven filters were removed from passenger cars during the "winter"/"summer" seasons. The taxonomic identification of the fungi isolated from the filters was performed using both the culture-based and molecular methods. RT-PCR was applied to assess the presence of gene fragments regulating aflatoxin biosynthesis in the isolates obtained from fungal cultures. The average fungal concentrations in the filter samples collected during the summer/winter season were 5.4 × 104 cfu/m2 and 2.4 × 104 cfu/m2, respectively. Most of the filter samples, collected in both the studied seasons, revealed the presence of Aspergillus species including A. fumigatus, A. niger, A. terreus and/or A. flavus. The recorded levels of fungal contamination of AC filters in passenger cars indicate the necessity for more frequent filter replacement in this type of vehicle. Occupational exposure to moulds and the resulting health problems that may be experienced by professional drivers should be properly recognised in order to undertake effective preventive measures.

Across-Shift Changes in Viable Nasal Bacteria among Waste-Incineration Plant Workers-A Pilot Study.

The aim of this pilot study was to assess the time-related changes in viable nasal bacteria concentrations among waste-incineration plant (WIP) workers compared to a group of office building (OB) workers outside the plant. In total, 20 volunteers participated in the study, including 14 WIP and 6 OB workers. WIP workers were divided into two sub-groups: supervisory staff (SVS) and maintenance and repair workers (MRW). Nasal swabs were collected before and after the morning work shift. Airborne bacteria were sampled with a six-stage impactor to assess the bioaerosol size distribution. The analysis showed that a significant, almost three-fold increase in nasal bacterial concentration was found only among WIP workers, and this referred mainly to anaerobic species. The load of anaerobic bacteria at the beginning of work was 12,988 CFU/mL, and after work shift 36,979 CFU/mL (p < 0.01). Significant increases in microbial concentrations was found only in the MRW subgroup, among non-smoking workers only. The results showed increased bacterial concentration in WIP nasal samples for as many as 12 bacterial species, including, e.g., Streptococcus constellatus, Peptostreptococcus spp., E. coli, and P. mirabilis. These preliminary data confirmed that the nasal swab method was helpful for assessment of the workers' real-time exposure to airborne bacteria.

Detection and identification of potentially infectious gastrointestinal and respiratory viruses at workplaces of wastewater treatment plants with viability qPCR/RT-qPCR.

This study aimed to qualitatively and quantitatively assess the prevalence of the most common respiratory and gastrointestinal viruses in the air, surface swab, and influent/effluent samples collected in wastewater treatment plants (WWTPs). Application of qPCR/RT-qPCR (quantitative polymerase chain reaction/reverse-transcription quantitative polymerase chain reaction) assays combined with PMA (propidium monoazide) dye pretreatment allowed detecting the potentially infectious and disintegrated viral particles in collected samples. In the air at workplaces in WWTPs, the most frequent isolation with the highest concentrations (reaching up to 103 gc/m3 of potentially infectious intact viral particles) were observed in case of adenoviruses (AdVs) and rotaviruses (RoVs), followed by noroviruses (NoVs). Viruses were significantly more often detected in the air samples collected with Coriolis µ impinger, than with MAS-100NT impactor. The temperature negatively (Spearman correlation: -1 < R < 0; p < 0.05), while RH (relative humidity) positively (0 < R < 1; p < 0.05) affected airborne concentrations of potentially infectious viral particles. In turn, the predominant viruses on studied surfaces were RoVs and noroviruses GII (NoV GII) with concentrations of potentially infectious virions up to 104 gc/100 cm2. In the cases of SARS-CoV-2 and presumptive SARS-CoV-2 or other coronaviruses, their concentrations reached up to 103 gc/100 cm2. The contamination level of steel surfaces in WWTPs was similar to this on plastic ones. This study revealed that the qualitative and quantitative characteristics of respiratory and gastrointestinal viruses at workplaces in WWTPs is important for proper exposure assessment and needs to be included in risk management in occupational environment with high abundance of microbial pollutants derived from wastewater.

Microbial contamination of money sorting facilities.

INTRODUCTION: Money is the most common item with which we have daily contact. Circulated banknotes and coins can become microbiologically contaminated and act as both a source and a means of spreading such pollutants. MATERIAL AND METHODS: The study was carried out in three money sorting facilities in Poland. Bioaerosol samples were collected using a 6-stage Andersen impactor, and microorganisms deposited on tabletop surfaces were sampled using the swab method. Bacterial and fungal concentrations were calculated and all isolated species were taxonomically identified. RESULTS: The study confirmed that means of payment are active sources of microbial emission in money sorting facilities. The bioaerosol concentrations did not exceed the threshold limit values proposed for this type of office premises. It confirms that ventilation systems in these facilities worked efficiently, protecting them from the migration of microbial contaminants present in both indoor and outdoor (atmospheric) background air. On the other hand, the average concentrations of bacteria and fungi on tabletop surfaces in banknote and coin sorting rooms were above the proposed purity levels for indoor surfaces and should be treated as microbiologically contaminated. Microbiota isolated from the air and surfaces were very diverse and among those strains were bacterial and fungal pathogens that can pose a health threat to exposed individuals. CONCLUSIONS: The results showed that employees in money sorting facilities were exposed to microorganisms that may contribute to the development of adverse health outcomes. To protect them, highly efficient hygienic measures should be introduced in this working environment, to prevent both unwanted pollution and subsequent secondary emission of microbial contaminants from sorted means of payment and tabletop surfaces.

Prevalence of Bovine Leukemia Virus (BLV) and Bovine Adenovirus (BAdV) genomes among air and surface samples in dairy production.

We aimed to assess the occurrence of bovine viruses (bovine leukemia virus-BLV and bovine adenovirus-BAdV) at workplaces in traditional dairies and to evaluate the potential role of airborne and surface contamination in spreading of these viruses derived from raw milk. The total amount of 122 samples-including 37 air (bioaerosol), 40 surface, and 45 milk samples-were checked for the presence of BLV and BAdV genomes using RT-qPCR/qPCR method. The study showed that the viruses were present in 7 air (among them 71.4% were BLV-positive and 28.6% were BAdV-positive), 14 surface (among them 85.7% were BLV-positive and 14.3% were BAdV-positive), and 34 milk (all were BLV-positive only) samples. Statistical analysis revealed that both the air and surfaces in studied occupational environment were more frequently contaminated with BLV than with BAdV (Chi-square test: p = 0.002, Fisher's Exact test: p = 0.002). Kruskal-Wallis tests showed significant differences in BLV genome concentrations in the air (p = 0.045) as well as in BLV and BAdV genome concentrations on surfaces (p = 0.005 and p = 0.040, respectively) between studied processing areas. In units of genome copies (gc) per area, the highest concentrations of BLV and BAdV genomes in the air (9.8 × 101 ± 1.14 × 102 gc/m3 and 5.4 × 101 ± 9.1 × 101 gc/m3, respectively) and on surfaces (9.83 × 102 ± 7.41 × 102 gc/100cm2 and 2.30 × 102 ± 3.8 × 102 gc/100cm2, respectively) were observed in milk reception area. The air and surfaces of pre-production zones were also significantly more contaminated with BAdV genomes compared to production areas (Mann-Whitney test: p = 0.039 and p = 0.029, respectively). This study showed that dairy workers may be exposed to bovine viruses through the inhalation of bioaerosols and contact with contaminated surfaces. To reduce the probability of virus transmission from the raw milk to humans, efficient surface cleaning procedures degrading viral particles should be introduced and the use of personal protection equipment, especially within pre-production zones, should be required. As the raw milk may be a source of bovine viruses, the development of strategies for both the control and eradication of BLV and BAdV among cattle seems to be also urgently needed.

Adverse health outcomes among workers of wood pellet production facilities.

INTRODUCTION: Workers of pellet production facilities (WPPF) are exposed to high concentrations of wood dust and microbial pollutants. Such stimulation may lead to numerous allergic and toxic reactions, infections, and other non-specific syndromes. OBJECTIVE: To check the influence of individual traits of workers and characteristic features / factors of their living and working environments on the probability of adverse outcomes' appearance. MATERIAL AND METHODS: The questionnaire study assessing adverse health effects resulting from individual exposure was conducted among 28 workers of 10 Polish WPPF. The logistic regression (for dichotomous variables) was used to determine the appearance probability (given as odds ratio) of adverse symptoms or diseases. RESULTS AND CONCLUSIONS: WPPF workers may have an increased risk of developing work-related adverse health outcomes. Both the individual traits and environmental exposure factors significantly influence the probability of their occurrence.

Microbial contamination level and microbial diversity of occupational environment in commercial and traditional dairy plants.

OBJECTIVE: The aim of this study was to assess microbial contamination of the air and surfaces at workplaces in commercial (CD) and traditional (TD) dairies. MATERIAL AND METHODS: Bioaerosol (impactor) and surface (swab) samples were collected in CD and in TD. Bacterial and fungal concentrations in the air and on surfaces were calculated and all isolated microorganisms taxonomically identified, based on their morphological, biochemical and molecular features. RESULTS: Average concentrations of bacterial aerosol ranged between 70-860 CFU/m3 and 265-14639 CFU/m3, while for fungal aerosol were between 50-290 CFU/m3 and 55-480 CFU/m3 in CD and TD, respectively. Average bacterial concentrations on surfaces ranged between 1.0-49.7 CFU/cm2 and 0.2-60.4 CFU/cm2, whereas average fungal surface contamination ranged between 0-2.7 CFU/cm2 and 0-4.6 CFU/cm2 in CD and TD, respectively. Qualitative analysis revealed mainly the presence of saprophytic microorganisms; however, several pathogenic strains (Staphylococcus aureus, Streptococcus intermedius, Clostridium perfringens, Actinomyces spp., Streptomyces spp., Candida albicans) were also isolated from both the air and surface samples in the studied dairies. CONCLUSIONS: The air and surfaces in TD were more polluted than those in CD; however, in both types of dairies, the levels of microbial contaminants did not exceed respective threshold limit values. Nevertheless, the presence of pathogenic microorganisms may increase health risk for dairy workers and influence the quality of products. Hence, proper hygienic measures should be introduced and performed to guarantee high microbial quality of both production processes and milk products.

Nasal lavage as analytical tool in assessment of exposure to particulate and microbial aerosols in wood pellet production facilities.

Occupational exposure to wood dust and bioaerosols may lead to numerous respiratory tract diseases. We aimed to assess a degree of workplace contamination with dust, bacteria, fungi, endotoxins, and (1 → 3)-ß-D-glucans released into the air during wood processing in pellet production facilities and to check against this background the usefulness of nasal lavage (NAL) as analytical tool for assessment of combined workers' exposure to airborne dust and microbiological contaminants. In 10 pellet plants, the particulate (wood dust) aerosol concentrations were determined by using Grimm aerosol spectrometer and CIS filter sampler. The collected CIS samples were subsequently used to evaluate endotoxin and (1 → 3)-ß-D-glucan concentrations. Simultaneously with particulate aerosol, bioaerosol samples were collected by using 6-stage Andersen and single-stage MAS impactors. Bacterial and fungal aerosol concentrations were calculated and all isolated microorganisms were taxonomically identified. NAL fluid samples were collected from workers exposed to studied aerosols and the concentrations of proinflammatory mediators (IL-1ß, IL-6, IL-8, and TNFα) and cytological image of nasal mucosa (expressed as cell counts) were established. The dynamics of production activities resulted in wide range of observed wood dust, microorganism, endotoxin and (1 → 3)-ß-D-glucan concentrations reaching periodically extremely high values up to 65 mg m-3, 19,320 CFU m-3, 215 ng m-3 and 1525 ng m-3, respectively. Environmental stress caused by exposure to particulate and microbial aerosols stimulated immune response among workers of pellet production facilities. Correlation analysis revealed that interleukin levels and the number of cells in NAL were significantly affected by both wood dust and bioaerosol concentrations. As nasal mucosa serves as the primary barrier against inhaled pollutants, NAL seems a reliable analytical material to assess work-related adverse respiratory health outcomes derived from such exposure.

Across-shift changes in upper airways after exposure to bacterial cell wall components.

INTRODUCTION: To assess the across-shift changes of cytokine concentrations in nasal lavage (NAL) samples were collected from workers exposed to bacterial cell wall components present in organic dust in three different occupational environments. MATERIAL AND METHODS: The study was conducted in 38 employees including 10 workers from a municipal waste sorting plant (WSP), 20 from a sewage treatment plant (STP) and 8 from an office building (OB), who were established as a reference group, not exposed to organic dust. Interleukins 1ß (IL-1ß), 6 (IL-6), 8 (IL-8) and tumour necrosis factor alpha (TNF-α) were examined in NAL before and after work shift on Wednesdays. Bioaerosol exposure was determined by personal measurements and analysed for organic dust, endotoxins (END) and peptidoglycans (PGN). RESULTS: The analysis included the results for IL-8 only, because for the other cytokines their concentrations in 80% of cases were below the detection level. The most polluted were the workplaces in WSP with average concentrations of organic dust - 3.47 mg/m3, END - 96.31 ng/m3 and PGN - 571.88 ng/m3. The results of IL-8 showed a significant difference between the studied groups after the work shift (p=0.007). Among WSP workers concentrations of IL-8 increased also significantly (p=0.015) during the work shift. Multivariate analysis showed that organic dust and END were the factors that in the most distinct way (p<0.001) influenced changes of IL-8 levels in NAL. CONCLUSIONS: Each alteration in the composition of bioaerosols will probably determine the changes in the mechanisms responsible for both formation and modulation of inflammatory reactions in exposed workers.

Bacterial aerosols in a municipal landfill environment.

Landfills collecting substantial amounts of municipal waste support multiplication of different bacteria mainly due to organic matter contained in the deposited materials. With time, they may become active emission sources of these microorganisms. Taking into account both occupational and public health and safety, there is an indisputable necessity to monitor the level of air contamination caused by both bacterial cells and their components (e.g., endotoxins). In this study, the concentrations of total viable bacteria (TVB), and Gram-negative bacteria (GNB), as well as their particle size distributions and concentrations of GNB endotoxins were assessed at various locations within the landfill area. The concentrations of TVB and GNB in the air samples changed depending on the season, location (i.e. active sector versus surroundings) and landfill activity level (i.e. exploitation or standstill periods). Higher abundances of endotoxins were found during the standstill period, and they were significantly correlated with organic dust concentrations. The microbial particle size distribution was associated with the landfill operational state, being predominated by fine below 4.7 µm and coarse fractions above 7.0 µm within the active sector during exploitation and standstill periods, respectively. These results supported by a spatial distribution of bacterial aerosol indicate a clear impact of operated landfill on microbiological air quality within the occupied location and nearby areas. Considering health and safety of landfill workers and neighboring residents, who can be exposed to airborne microbial pollutants, repeated bioaerosol monitoring need to be established. It should facilitate both a special planning within the landfill area and undertaking preventive actions in its near and distant surroundings.

Microbiological air quality in office buildings equipped with dventilation systems.

Proper hygienic conditions in office buildings are of a high importance for both health and well-being of the employees. The aim of this study was the direct comparison of different ventilation systems on microbiological environments in 15 office buildings. The results showed that both bacterial and fungal concentrations in the naturally ventilated office buildings were between 70 and 1600 cfu/m3 , while in the offices equipped with air-conditioning and mechanical ventilation systems, concentrations were lower, that is, between 10 and 530 cfu/m3 and 20 and 410 cfu/m3 , respectively. The size distribution analysis revealed that microorganisms were present in the air mainly as single cells (1.1-3.3 µm) and large aggregates (4.7->7 µm). If deposited in the human respiratory tract, they may be responsible for nose and eyes irritations, asthmatic reactions, and allergic inflammations. The most prevalent bacterial species indoors were Gram-positive cocci (mainly from Staphylococcus and Micrococcus/Kocuria genera) and endospore-forming Gram-positive rods (from Bacillus genus). Among the most common fungal species were those from genera Penicillium, Aspergillus, and Cladosporium. Effectively working and regularly maintained mechanical ventilation or air-conditioning systems ensure a better hygienic quality in the office buildings than natural/gravitational ventilation.

[Assessment of fungal aerosol exposure at selected workplaces contaminated with organic dust of different origin]. / Ocena narazenia na aerozol grzybowy na wybranych stanowiskach pracy zanieczyszczonych pylem organicznym o róznym pochodzeniu.

BACKGROUND: In recent years, the number of people suffering from diseases caused by fungi has been increasing. However, knowledge of the biodiversity of fungal pathogens in the work environment is still insufficient. The aim of this work was to evaluate the exposure to fungi being disseminated in the air of workplaces contaminated with organic dust of plant and animal origin. MATERIAL AND METHODS: Bioaerosol samples were collected at 3 occupational settings (poultry farm, biomass burning power plant and wastewater treatment plant) using button samplers. Quantitative and qualitative analysis of fungal aerosol was conducted by employing macro- and microscopic methods. Selected strains were then studied by polymerase chain reaction (PCR) using srodointernal transcribed spacers (ITS): ITS1-ITS2, ITS3-ITS4 and ITS1-ITS4 primer pairs. RESULTS: Average concentrations of fungal aerosol at workplaces ranged 1.2×102-2.1×106 cfu/m3. The highest fungal concentrations were recorded in the poultry farm, while the lowest were noted at the wastewater treatment plant. Aspergillus fumigatus was a predominant component of the mycobiota in the power plant and wastewater treatment plant. Almost 100% identification agreement of this pathogen between the traditional and molecular method was noted. CONCLUSIONS: The fungal concentrations in poultry farms exceeded the Polish proposal for the threshold limit value (5×104 cfu/m3). The results of the study demonstrate a high compatibility of A. fumigatus' identification using the traditional and molecular methods. Taking into account the fact, that a long term exposure to A. fumigatus conidia at workplaces may result in numerous health complaints, the use of proper protective equipment by workers must be a standard procedure. Med Pr 2018;69(3):269-280.

Anaerobic bacteria in wastewater treatment plant.

PURPOSE: The objective of this study was to assess exposure to anaerobic bacteria released into air from sewage and sludge at workplaces from a wastewater treatment plant (WWTP). METHODS: Samples of both sewage and sludge were collected at six sampling points and bioaerosol samples were additionally collected (with the use of a 6-stage Andersen impactor) at ten workplaces covering different stages of the technological process. Qualitative identification of all isolated strains was performed using the biochemical API 20A test. Additionally, the determination of Clostridium pathogens was carried out using 16S rRNA gene sequence analysis. RESULTS: The average concentration of anaerobic bacteria in the sewage samples was 5.49 × 104 CFU/mL (GSD = 85.4) and in sludge-1.42 × 106 CFU/g (GSD = 5.1). In turn, the average airborne bacterial concentration was at the level of 50 CFU/m3 (GSD = 5.83) and the highest bacterial contamination (4.06 × 103 CFU/m3) was found in winter at the bar screens. In total, 16 bacterial species were determined, from which the predominant strains belonged to Actinomyces, Bifidobacterium, Clostridium, Propionibacterium and Peptostreptococcus genera. The analysis revealed that mechanical treatment processes were responsible for a substantial emission of anaerobic bacteria into the air. In both the sewage and air samples, Clostridium perfringens pathogen was identified. CONCLUSIONS: Anaerobic bacteria were widely present both in the sewage and in the air at workplaces from the WWTP, especially when the technological process was performed in closed spaces. Anaerobic bacteria formed small aggregates with both wastewater droplets and dust particles of sewage sludge origin and as such may be responsible for adverse health outcomes in exposed workers.

Prevalence of Human Parainfluenza Viruses and Noroviruses Genomes on Office Fomites.

The aim of this study was to evaluate the potential role of office fomites in respiratory (human parainfluenza virus 1-HPIV1, human parainfluenza virus 3-HPIV3) and enteric (norovirus GI-NoV GI, norovirus GII-NoV GII) viruses transmission by assessing the occurrence of these viruses on surfaces in office buildings. Between 2016 and 2017, a total of 130 surfaces from open-space and non-open-space rooms in office buildings located in one city were evaluated for HPIV1, HPIV3, NoV GI, and NoV GII viral RNA presence. Detection of viruses was performed by RT-qPCR method. Study revealed 27 positive samples, among them 59.3% were HPIV3-positive, 25.9% HPIV1-positive, and 14.8% NoV GII-positive. All tested surfaces were NoV GI-negative. Statistical analysis of obtained data showed that the surfaces of office equipment including computer keyboards and mice, telephones, and desktops were significantly more contaminated with respiratory viruses than the surfaces of building equipment elements such as door handles, light switches, or ventilation tracts (χ 2 p = 0.006; Fisher's Exact p = 0.004). All examined surfaces were significantly more contaminated with HPIVs than NoVs (χ 2 p = 0.002; Fisher's Exact p = 0.003). Office fomites in open-space rooms were more often contaminated with HPIVs than with NoVs (χ 2 p = 0.016; Fisher's Exact p = 0.013). The highest average concentration of HPIVs RNA copies was observed on telephones (1.66 × 102 copies/100 cm2), while NoVs on the light switches (1.40 × 102 copies/100 cm2). However, the Kruskal-Wallis test did not show statistically significant differences in concentration levels of viral RNA copies on surfaces between the all tested samples. This study unequivocally showed that individuals in office environment may have contact with both respiratory and enteric viral particles present on frequently touched surfaces.

Monitoring of bacterial pathogens at workplaces in power plant using biochemical and molecular methods.

PURPOSE: The aim of this study was to characterize the ways of spreading of the most common bacterial species isolated from workers as well as from the air and raw materials at the workplaces in power plant utilizing biomass sources. To monitor microbial transmission and identify the source of contamination in the working environment, a combination of molecular and biochemical methods was applied. METHODS: The study was carried out at workplaces in power plant utilizes biomass as a main fuel source. At each of the studied workplaces, bioaerosol particles were collected on sterile Teflon filters using personal conical inhalable samplers (CIS), and biomass samples (straw pellets and briquettes, corn briquettes, sunflower pellets and wood chips) were directly taken from their storage places. Simultaneously with that, the swab samples from the hands of ten workers and their used respiratory masks (of FFP2 class) were also collected after the work shift to evaluate individual workers' microbial contamination. In all collected samples, total bacterial concentrations were assessed and the most common microbial isolates were identified to the species level using both biochemical (API tests) and molecular polymerase chain reaction (PCR), followed by random amplification of polymorphic DNA (RAPD) typing methods. RESULTS: The mean concentrations of culturable bacteria in the air and in biomass samples at the studied workplaces were high, i.e. 1.2 × 106cfu/m3 and 3.8 × 104cfu/g, respectively. The number of bacteria in the swab and mask samples also reached a high level of 1.4 × 104 cfu/ml and 1.9 × 103 cfu/cm2, respectively. Among the most frequently isolated microorganisms from all types of samples were Gram-positive bacteria of the genus Bacillus and Staphylococcus xylosus. 37 bacterial strains belonging to the genus Bacillus (B. licheniformis 8, B. pumilus 15 and B. subtilis 4) and Staphylococcus (10) were genotyped by the RAPD-PCR method. Based on RAPD-PCR analyses, the genomic similarity among 19 Bacillus strains isolated from biomass, air, protective mask and hand samples as well as 6 S. xylosus strains isolated from air, mask and hand samples exceeded 80%. CONCLUSION: This study demonstrated that biomass is the primary source of bacteria at power plant workplaces. These results also revealed that biomass-associated bacteria can be easily transferred to workers' hands and mask during their routine activities. To improve health protection at the workplaces, adequate training courses on hand hygiene and how to use and remove respiratory masks correctly for workers should be introduced as a key element of the prevention strategy. From the occupational point of view, the PCR-based methods seem to be an efficient tool for a fast and precise typing of bacterial strains isolated from different sources in the occupational environment. Such methods may help to implement appropriate prophylactic procedures and minimize transmission of infectious agents at workplaces.

Exposure to culturable and total microbiota in cultural heritage conservation laboratories.

OBJECTIVES: To date, the scientific source materials usually focus on microbial contamination of the museum or library collections themselves, while the exposure of persons who professionally deal with this type of objects in cultural heritage conservation laboratories is ignored. MATERIAL AND METHODS: The study was carried out in 9 naturally ventilated conservation laboratories with no history of water damage. Viable (understood as culturable) bioaerosol stationary samples were collected in both outdoor and indoor environments using 6-stage Andersen impactor. Simultaneously, stationary and personal indoor bioaerosol measurements were carried out using both Gesamtstaubprobenahme an der Person (GSP) and Button filter samplers. These measurements were complemented by evaluation of microbial content in the dust settled on conserved works of art. All impactor, filter, and settled dust samples were quantitatively examined to obtain viable and total concentrations of bacteria and fungi. All isolated microbial strains were taxonomically identified. RESULTS: At workplaces, the concentrations of viable microorganisms in air were below 2000 cfu/m3 and accounted for not more than 5.5% of total microbiota. The study showed that quantitative assessment of viable bioaerosol can be made with an Andersen impactor as well as by using Button and GSP filter samplers, irrespective of whether they are applied for personal or stationary measurements. Compared to the impactor, however, the use of filter samplers for microbial contamination monitoring substantially limits the scope of qualitative information which can be obtained. Size distribution analysis revealed that the largest "load" of microorganisms can penetrate into the respiratory tract between the trachea and terminal bronchi, and thereby may be responsible for allergic inflammations in exposed workers. CONCLUSIONS: The precise assessment of microbial hazards in conservation laboratories should comprise control of both viable and total particle counts. The hermetization of such workplaces and control of relative humidity should be implemented and maintained to assure proper hygienic conditions.