[The use of roncoleukin for LAK therapy in ovarian cancer].

Roncoleukin--recombinant IL-2--was studied with a view to application for LAK therapy. Lymph of ductus thoracicus from ovarian cancer patients receiving adjuvant autolymphochemotherapy was used as source of lymphocytes. Immunobiologic activity of lymphocytes was assayed after 30-minutes incubation with roncoleukin and 24- or 48-hours culturing. The investigation established an increase in T-cell proliferation, autocrine stimulation of IL-2 production, growth of NK-cells cytotoxicity, immediate cytotoxic effect on K562 target cells culture and such sensitizing effect as roncoleukin's potential to boost its susceptibility to NK-lysis. LAK-therapy was used as a component of complex treatment of ovarian cancer (8). Our method has a future in dealing with the present pathology.

[Changes in lymphocyte and erythrocyte membrane microviscosity in cancer patients]. / Izmenenie mikroviazkosti membran limfotsitov i éritrotsitov krovi u onkologicheskikh bol'nykh.

Lymphocyte and erythrocyte membrane microviscosites of lipid-protein contact zones and lipid bilayer in blood of patients with gastric, urinary bladder and ovary cancers were investigated in comparison with healthy volunteers by means of coefficients of eximerization of fluorescent probe pyrene. The decrease of membrane fluidity (i.e. the increase of microviscosity) was revealed in blood cell membranes of patients with diffuse cancers of studied localizations. In the case of ovary cancer membrane fluidity decreased significantly only in patients with ascite form of the disease. The most pronounced increase of microviscosity was observed in lymphocytes and especially in erythrocytes of practically incurable patients. Interrelationship between microviscosity and other processes that influence on structural properties of membranes under malignancy and their functional role are discussed.

[A method of fluorescence spectral analysis of lymphocyte function in cancer patients]. / Metod fliuorestzentno-spektral'nogo analiza funktsional'noi aktivnosti limfotsitov u onkologicheskikh bol'nykh.

A luminescence microspectral examination of lymphocytes in peripheral blood sampled from ovarian tumor patients has shown spectral pattern, and, particularly, parameter alpha (ratio of one-spiral to two-spiral nucleic acids in cell) and parameter alpha distribution pattern to vary in the course of treatment: as efficacy increases, the lymphocyte fluorescence spectrum shows two peaks (lambda = 530 and 640 nm) of nearly identical intensity, parameter alpha grows, too, and its distribution shifts to the right. This mirrors an activation of synthetic and functional processes going on in peripheral blood lymphocytes of patients and points to enhanced non-specific resistance.

[Aspiration biopsy of pathological formations of the abdominal cavity and retroperitoneal space]. / Aspiratsionnaia punktsionnaia biopsiia patologicheskikh obrazovanii briushnoi polosti i zabriushinnogo prostranstva.

Aspiration diagnostic puncture of a three-dimensional structure in the retroperitoneal space and abdominal cavity palpated through the anterior abdominal wall was performed in 58 patients. The puncture was carried out in cases of pathological processes arising from the stomach, large intestine, pancreas, kidneys, adrenal gland, ovaries, retroperitoneal lymph nodes, mesenchyma, and smooth-muscle tissue, and in appendicular and paravesical abscesses. The findings of cytological examination correctly pointed out the nature of the pathological process in 78% of cases. There were no false-positive results, false-negative results were encountered in 9 out 46 patients. The cytological data allowed the clinical diagnosis to be made precisely in cases in which the organic nature of the three-dimensional structure was doubtful. The expected complications due to possible damage of hollow organs and blood vessels by the needle were not encountered. Metastatic spreading of the tumor which could have been caused by the diagnostic manipulations also did not occur.