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3.
Zh Mikrobiol Epidemiol Immunobiol ; (7): 119-23, 1976 Jul.
Artigo em Russo | MEDLINE | ID: mdl-188277

RESUMO

In gel-filtration of Cl. perfringens type A toxin on Sephadex F-100 and F-50 there was revealed relationship between the leukotoxic activity and a high-molecular component. A method of ion-exchange chromatography on a column with DEAE-Sephadex A-25 from the Cl. perfringens toxin there were obtained 8 fractions 3 of which possessed a marked leukotoxic activity: the capacity to destroy neutrophils in the Svejcar and Vancurik test and to-depress the phagocytic activity of leukocytes. Lecithinase and lethal activity was revealed in one of these fractions only. Leukotoxic fractions differed by the capacity to destroy neutrophils and to decrease their phagocytic activity.


Assuntos
Clostridium perfringens , Leucócitos/efeitos dos fármacos , Toxinas Biológicas/toxicidade , Células Cultivadas , Cromatografia em Gel , Cromatografia por Troca Iônica , Clostridium perfringens/análise , Testes Imunológicos de Citotoxicidade , Humanos , Neutrófilos/efeitos dos fármacos , Fagocitose/efeitos dos fármacos , Toxinas Biológicas/isolamento & purificação
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