[Factors of pathogenicity of Сorynebacterium non-diphtheria, Isolated from patients with the pathology of the respiratory tract.]

Сorynebacteria non-diphtheria of С. pseudodiphtheriticum strains, despite the absence of the ability to produce toxin, can be associated with the development of inflammatory diseases of the respiratory and urogenital tract, skin, purulent-septic processes of various localization, etc. This indicates the presence of other pathogenicity factors, in addition to toxin, which may be adhesive and invasive activity. Characteristics of pathogenicity factors (adhesiveness and invasiveness) of Corynebacteria non-diphtheria isolated from patients with pathology of the respiratory tract. The strains of Corynebacteria non-diphtheria (38) isolated from the upper respiratory tract from patients with chronic tonsillitis (C. pseudodiphtheriticum - 9) and angina (C. pseudodiphtheriticum - 14 pcs.), As well as practically healthy subjects (C. pseudodiphtheriticum - 15 pcs.). The ability for adhesion and invasion of corynebacteria was studied on the culture of the cells of the pharyngeal epithelium Hep-2 carcinoma. The number of corynebacteria, adherent and invaded on Hep-2 cells, was determined by sowing the flush with 20% serum agar, followed by counting the average number of colony forming units (CFU) per 1 ml. Electron microscopic investigation of adhesion and invasion of corynebacteria on the culture of Hep-2 cells was carried out by transmission electron microscopy. The adhesiveness of strains of C. pseudodiphtheriticum isolated from practically healthy individuals was lower (p≤0,05) than that of all the investigated strains of Corynebacteria non-diphtheria isolated from patients with pathology of the respiratory tract. The most pronounced adhesive properties (238.3±6.5 CFU/ml) were found in C. pseudodiphtheriticum strains isolated from patients with angina compared with those isolated from patients with chronic tonsillitis. Adhesiveness and invasiveness in all strains studied had a positive correlation. Electron microscopic examination shows corynebacteria, both adherent to the surface of Hep-2 cells and accumulated contrast medium, and invasive, electron-transparent. Corynebacteria non-diphtheria of C. pseudodiphtheriticum strains isolated from patients with respiratory tract pathology (angina, chronic tonsillitis) had a higher ability to adhere and invade than C. dodiphtheriticum strains isolated from practically healthy individuals. The pronounced ability for adhesion and invasion, considered as pathogenicity factors of C. pseudodiphtheriticum, allows them to realize their pathogenic potential, protecting against the action of the host's immune system and antibacterial drugs.

[EFFECT OF ANTIBACTERIAL PREPARATIONS ON VIBRIO CHOLERAE EL TOR BIOFILMS].

AIM: Study the effect of antibacterial preparations on biofilms of Vibrio cholerae El Tor. MATERIALS AND METHODS: Sensitivity of V cholerae El Tor (6 strains) to various concentrations of antibacterial preparations (doxycycline,.tetracycline, levomycetin, rifampicin, gentamycin, ceftazidime) was determined (MD 4.2.2495-09). Transmission electron microscopy was used for visualization of the effect of preparations on biofilms. RESULTS: The values of minimal inhibiting concentrations of antibacterial preparations against biofilms have increased by 5 - 100 times compared with plankton cultures. Certain smoothing of strands between the bacterial cell and substrate, alteration of vibrios' form, reduction of electron density of the matrix with an increase of its transparency were observed during electron-microscopy of the effect of antibacterial preparations on the biofilm. CONCLUSION: Study of the effect of antibacterial preparations on biofilms could increase effectiveness of rational antibiotics therapy of infec- tions by selection of preparations that disrupt functioning of microbial communities.

[The analysis of biofilms Vibrio choleraе using technique of transmissive electronic microscopy.]

The cholera continues to be one of the priority problems of world health care that determines necessity of its permanent monitoring and also further investigation of this infection in microbiological and ecological aspects. The phenomenon of biofilm formation plays a significant role among mechanisms permitting comma bacillus year by year to retain its ecological niche and to spread in various basins over a period of decades. The application of techniques of electronic microscopy played a decisive role in studying the process of biofilm formation. The transmission electronic microscopy has a number of significant advantages, but studying of biofilms using the given technique has certain limitations and peculiarities affecting derivable results. The purpose of study is modeling of biofilms of comma bacillus using a new original technique and analyzing them implementing transmission electronic microscopy. An original substrate was developed to cultivate biofilms of comma bacillus adjusted to convenient sampling preparation for transmission electronic microscopy. The application of the given technique manifold simplifies the process sampling preparation and permits to retain at most the native structure of biofilms. By means of combining alternatives of contrasting and comparing of obtained results the role of various components in the process of biofilm formation is determined. Also, the structure of matrix of biofilms and morphologic phasic picture of process of biofilm formation of comma bacillus is demonstrated. The electronic microscopic indications of biofilm forms of comma bacillus are marked out. The presented data supports optimization of studying biofilms using transmission electronic microscopy and better understanding phenomenon of biofilm formation.

[The indices of the blood serum lipid and hormonal spectra in patients with diffuse neurodermatitis]. / Nekotorye pokazateli lipidnogo i gormonal'nogo spektrov syvorotki krovi u bol'nykh diffuznym neirodermitom.

Thirty-two female patients suffering from neurodermatitis were examined. Increased hydrocortisone level and reduced estrogen concentration in the blood serum were observed during exacerbation of the condition. During remission these parameters normalized. Exacerbations were associated with marked dyslipidemia at the expense of elevated concentrations of triglycerides, free cholesterol, low-density lipoproteins in the blood serum; these parameters also showed a tendency to normalization during remission. A correlation could be traced between hormonal imbalance and disturbances of lipid metabolism regulated by these hormones. The authors emphasize the necessity of correcting dyslipidemia during exacerbation of diffuse neurodermatitis.