RESUMO
Spore-forming bacteria with the optimum growth temperature from 40 to 65 degrees C were isolated from hot springs of Kamchatka with the temperature 50-93 degrees C and pH 6.3-7.3: Bacillus stearothermophilus, B. brevis, B. subtilis, B. cereus, B. megaterium, and B. licheniformis. The bacterial have a high biochemical activity and play therefore an important role in decomposition of organic substances.
Assuntos
Bactérias/isolamento & purificação , Temperatura Alta , Microbiologia da Água , Amilases/metabolismo , Bacillus/enzimologia , Bacillus/crescimento & desenvolvimento , Bacillus/isolamento & purificação , Água Doce , Bactérias Aeróbias Gram-Negativas/enzimologia , Bactérias Aeróbias Gram-Negativas/crescimento & desenvolvimento , Bactérias Aeróbias Gram-Negativas/isolamento & purificação , Peptídeo Hidrolases/metabolismo , Sibéria , Especificidade da Espécie , Esporos Bacterianos/crescimento & desenvolvimentoRESUMO
The thermotolerant culture of Thermoactinomyces vulgaris PA II-4A was cultivated in the Biotec fermenter to obtain the enzyme preparation with a high proteolytic and lytic activity. Resistance of the cells of Candida utilis and Saccharomyces fragilis (mesophilic and thermotolerant strains) to the lytic action of the enzyme preparation was different. Preliminary treatment of the yeast cells by L-cysteine increased their susceptibility to the lytic action of the preparation. The degree of lysis of the cells depended also on their age: the cells growing during 10-12 hours were lysed easier than the cells cultivated during 24 hours.