RESUMO
The review is dedicated to several aspects of sugar beet (Beta vulgaris L.) biotechnology: in vitro cultivation, callus induction, plant regeneration and genetic transformation. Media composition, methods of plant regeneration via somatic embryogenesis and protoplast culture are analysed. The use of Agrobacterium tumefaciens and gold particle bombardment is the base for modern genetic transformation methods.
Assuntos
Beta vulgaris/genética , Plantas Geneticamente Modificadas/genética , Transformação Genética , Beta vulgaris/crescimento & desenvolvimento , DNA de Plantas , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , ProtoplastosRESUMO
The results of the author's data and those from literature on ecology and genetic exchange of Bacillus thuringiensis strains have been analyzed in this brief review. It has been concluded that there is no strict confinement of B. thuringiensis strains of the particular serotypes to certain habitat and that the bacteriocinogenicity is of great importance in the intraspecies competition and stability of strains in nature. It has been emphasized that the plasmid conjugation process in B. thuringiensis can serve as a main factor of genetic variability to establish ecological placement of these bacteria.
