Investigating the joint application of negative pressure wound treatment and tissue therapy for chronic wounds in patients with diabetes.

This study aimed to investigate the effectiveness of combined negative pressure wound therapy (NPWT) and human amniotic membrane in patients with chronic wounds associated with diabetes. A total of five patients with type 2 diabetes, including ischemic and mixed forms of diabetic foot syndrome, presenting with ischemic wounds of the lower extremities were included in this study. Patients with uncorrected limb ischemia were excluded. The treatment protocol included diabetes compensation (treatment with fractional insulin therapy), anticoagulant, metabolic therapy and angiotropic therapy, physical treatment methods, osteoporosis therapy with calcium preparations, and wound-specific interventions. The primary treatment approach involved the application of a vacuum bandage to the transplanted human amniotic membrane, which improved the adaptation of the flap to the wound surface, allowed the removal of excess wound exudate, and stimulated angiogenesis and reparative properties. The combined approach of NPWT and biotherapy was a safe and effective cure for diabetic wounds, promoting faster wound healing, reducing the need for autodermoplasty, and possibly reducing the necessity for high-level amputations.

Cryopreservation: an optimizing factor for therapeutic potential of fetoplacental complex products.

The therapeutic potential of cryopreserved fetoplacental complex cells (FPCP) has been explored for the treatment of autoimmune diseases, including autoimmune hemolytical anemia (AIHA). In this study, a murine AIHA model was utilized to investigate the application of cryopreserved fetal liver cells (cFLC), from varying gestation intervals (14 and 19 postcoital days) to correct the functional state of the lymphohemopoietic complex (LHPC). The results demonstrate that cryopreservation had little negative effect on FLC-14 corrective ability from that of nonfrozen FLC-14. Cryopreservation of FLC-19 (later gestation period) resulted in an increase in functional corrective potential over that of matched controls. Further, the increase in corrective effect of the cFLC-19 was found to be similar to that of both FLC-14 populations. In summary, these results demonstrate the potential for the use of cryopreserved FLCs as a therapeutic option for the treatment of AIHA.