[Radial asymmetric keratotomy in mild-degree myopia]. / Radial'naia asimmetrichnaia keratotomiia pri miopii slaboi stepeni.

The suggested method for surgical correction of weak myopia involves two mutually perpendicular incision on the cornea in the major meridians. These two incisions result in but a slight alteration of the tissues, and a stable refraction effect of about 1.5 diopters is achieved at once; vision acuity improves to make 0.9-1.0.

Human CD4 binds immunoglobulins.

T cell glycoprotein CD4 binds to class II major histocompatibility molecules and to the human immunodeficiency virus (HIV) envelope protein gp120. Recombinant CD4 (rCD4) bound to polyclonal immunoglobulin (Ig) and 39 of 50 (78%) human myeloma proteins. This binding depended on the Fab and not the Fc portion of Ig and was independent of the light chain. Soluble rCD4, HIV gp120, and sulfated dextrans inhibited the CD4-Ig interaction. With the use of a panel of synthetic peptides, the region critical for binding to Ig was localized to amino acids 21 to 38 of the first extracellular domain of CD4. CD4-bound antibody (Ab) complexed with antigen approximately 100 times better than Ab alone. This activity may contribute to the Ab-mediated enhancement of cellular HIV interaction that appears to depend on a trimolecular complex of HIV, antibodies to gp120, and CD4.

Retinoic acid inhibition of transplasmalemma diferric transferrin reductase.

All trans retinoic acid inhibited diferric transferrin reduction by HeLa cells. The NADH diferric transferrin reductase activity of isolated liver plasma membranes was also inhibited by retinoic acid. Retinol and retinyl acetate had very little effect. Transplasma membrane ferricyanide reduction by HeLa cells and NADH ferricyanide reductase of liver plasma membrane was also inhibited by retinoic acid, therefore the inhibition was in the electron transport system and not at the transferrin receptor. Since the transmembrane electron transport has been shown to stimulate cell growth, the growth inhibition by retinoic acid thus may be based on inhibition of the NADH diferric transferrin reductase.

Two distinct events in lineage establishment of a biopotential hemopoietic cell line.

HL-60, a human myelomonocytic cell line can be induced to differentiate along either granulocyte or monocyte pathways and therefore is a good model to study lineage establishment. We have shown that there are two classes of reactions in the establishment of the granulocytic line distinguishable by their kinetics. The fast reaction occurs in hours and is induced by ouabain or A23187, agents which effect ions. The slow reactions occur at a constant rate over a period of 5 days and are induced by the surrogate inducers RA and D3 but also by the natural inducer GM-CSF. GM-CSF induces the enzyme markers which we use to characterize both granulocytes and monocytes in the same cell. If these markers are indeed unique to each of the lines this means that HL-60 has both gene programs initiated by GM-CSF and D3. Variant lines which do not respond to one or more of the inducers can be used to analyze this point since variants which are induced only to granulocytes or only to monocytes by GM-CSF have been isolated.