Reconstruction of Z-pinch emission spectra in the wavelength range of less than 10 Å using a crystal X-ray spectrograph.

This work is devoted to the reconstruction of Z-pinch plasma emission spectra in the wavelength range of less than 10 Å recorded by using a crystal x-ray spectrograph at the Angara 5-1 mega-ampere facility. The spectrograph JA-1 used in experiments has a cylindrical mica crystal with dimensions of 50 × 40 mm2 and radius of curvature of 100 mm. Registration of spectra is performed on the photographic film UF-4 with dimensions of 30 × 10 mm2. To reconstruct the spectra, the previously developed method based on iterative approximation of a true spectrum shape while minimizing a residual between experimental and calculated spectrograms is used. The calculated spectrogram was obtained taking into account the instrumental function of the spectrograph. To define the instrumental function a virtual Monte-Carlo model in the Geant4 toolkit has been developed. This model takes into account the interaction of radiation with the mica crystal using dynamical theory of diffraction. A true spectrum of Z-pinch plasma radiation is reconstructed for a 16 mm high load made of two nested cylindrical wire liners. External liner with a diameter of 12 mm has 40 Al wires with a diameter of 18 µm. The internal liner with a diameter of 5 mm has 4 W wires with a diameter of 6 µm. The W wires have a sputtered layer of Re that is 0.5 µm thick.

Single-pass method for reconstruction of extreme UV spectra.

This work is devoted to the development of a method for the reconstruction of plasma extreme UV (EUV) spectra recorded by a three frame grazing incidence spectrograph (GIS-3D). The spectrograph provides registration of radiation reflected from the diffraction grating (DG) on a three-frame detector based on a microchannel plate with a scintillator screen and registration on a CCD camera, with an exposure time of one frame of ∼1.5 ns. DG has a gold-coated spherical concave form with a radius of curvature of 2 m and dimensions of 30 × 40 × 10 mm3. In this case, radiation is incident on the DG at a grazing angle of 2°; the DG period is 1.66 µm. The new single-pass method for the reconstruction of plasma EUV spectra was developed, which solves the inverse problem of decomposing experimental signals into separate contributions from each of the diffraction orders, followed by the reconstruction of the true plasma spectrum. Using the developed method, the possibility of finding a close approximation to the shape of a DG groove profile based on a priori information about the recorded spectra was demonstrated. In order to test and demonstrate the efficiency of this method, several experimental EUV spectra obtained at the Z-pinch facility Angara-5-1 with a current of ∼3-4 MA through loads made of either tungsten wires or polypropylene fibers were reconstructed. In addition, to test the single-pass method, the transmittance of EUV in cold aluminum was measured in the wavelength range of 3-35 nm, and it has a good match with the Henke database.

Benchmark Experiment to Prove the Role of Projectile Excited States Upon the Ion Stopping in Plasmas.

We report on a precision energy loss measurement and theoretical investigation of 100 keV/u helium ions in a hydrogen-discharge plasma. Collision processes of helium ions with protons, free electrons, and hydrogen atoms are ideally suited for benchmarking plasma stopping-power models. Energy loss results of our experiments are significantly higher than the predictions of traditional effective charge models. We obtained good agreement with our data by solving rate equations, where in addition to the ground state, also excited electronic configurations were considered for the projectile ions. Hence, we demonstrate that excited projectile states, resulting from collisions, leading to capture-, ionization-, and radiative-decay processes, play an important role in the stopping process in plasma.

In vitro Reconstitution of the S. aureus 30S Ribosomal Subunit and RbfA Factor Complex for Structural Studies.

Ribosome-binding factor A (RbfA) from Staphylococcus aureus is a cold adaptation protein that is required for the growth of pathogenic cells at low temperatures (10-15°C). RbfA is involved in the processing of 16S rRNA, as well as in the assembly and stabilization of the small 30S ribosomal subunit. Structural studies of the 30S-RbfA complex will help to better understand their interaction, the mechanism of such complexes, and the fundamental process such as 30S subunit assembly that determines and controls the overall level of protein biosynthesis. This article describes protocols for preparation of RbfA and the small 30S ribosomal subunits and reconstitution and optimization of the 30S-RbfA complex to obtain samples suitable for cryo-electron microscopy studies.

[Elongation Factor P: New Mechanisms of Function and an Evolutionary Diversity of Translation Regulation].

The protein synthesis in cells occurs in ribosomes, with the involvement of protein translational factors. One of these translational factors is the elongation factor P (EF-P). EF-P is a three-domain protein that binds between the P and E sites of the ribosome, near the P-tRNA, the peptidyl transferase center, and E-site codon of the mRNA. The majority of studies showed that the EF-P helps the ribosome to synthesize stalling amino acid motifs, such as polyprolines. In the first part of this review, we inspect the general evolutionary variety of the EF-P in different organisms, the problems of the regulation provided by the EF-P, and its role in the sustainability of the protein balance in the cell in different physiological states. Although the functions of the EF-P have been well studied, there are still some problems that remain to be solved. The data from recent studies contradict the previous theories. Consequently, in the second part, we discuss the recent data that suggest the involvement of the EF-P in each translocation event, not only in those related to poly-proline synthesis. This activity contradicts some aspects of the known pathway of the removal of the E-tRNA during the translocation event. In addition, in the third part of this review, we tried to partly shift the interest from the antistalling activity of domain I of the EF-P to the action of domain III, the functions of which has not been closely studied. We expand on the idea about the involvement of domain III of the EF-P in preventing the frameshift and debate the EF-P's evolutionary history.

Dynamics of supernova bounce in laboratory.

We draw attention to recent high-explosive (HE) experiments which provide compression of macroscopic amount of matter to high, even record, values of pressure in comparison with other HE experiments. The observed bounce after the compression corresponds to processes in core-collapse supernova explosions after neutrino trapping. Conditions provided in the experiments resemble those in core-collapse supernovae, permitting their use for laboratory astrophysics. A unique feature of the experiments is compression at low entropy. The values of specific entropy are close to those obtained in numerical simulations during the process of collapse in supernova explosions, and much lower than those obtained at laser ignition facilities, another type of high-compression experiment. Both in supernovae and HE experiments the bounce occurs at low entropy, so the HE experiments provide a new platform to realize some supernova collapse effects in laboratory, especially to study hydrodynamics of collapsing flows and the bounce. Due to the good resolution of diagnostics in the compression of macroscopic amounts of material with essential effects of nonideal plasma in EOS, and observed development of 3D instabilities, these experiments may serve as a useful benchmark for astrophysical hydrodynamic codes.

Commissioning of the PRIOR proton microscope.

Recently, a new high energy proton microscopy facility PRIOR (Proton Microscope for FAIR Facility for Anti-proton and Ion Research) has been designed, constructed, and successfully commissioned at GSI Helmholtzzentrum für Schwerionenforschung (Darmstadt, Germany). As a result of the experiments with 3.5-4.5 GeV proton beams delivered by the heavy ion synchrotron SIS-18 of GSI, 30 µm spatial and 10 ns temporal resolutions of the proton microscope have been demonstrated. A new pulsed power setup for studying properties of matter under extremes has been developed for the dynamic commissioning of the PRIOR facility. This paper describes the PRIOR setup as well as the results of the first static and dynamic proton radiography experiments performed at GSI.

First biological images with high-energy proton microscopy.

High-energy proton microscopy provides unique capabilities in penetrating radiography including the combination of high spatial resolution and field-of-view, dynamic range of density for measurements, and reconstructing density variations to less than 1% inside volumes and in situ environments. We have recently proposed to exploit this novel proton radiography technique for image-guided stereotactic particle radiosurgery. Results of a first test for imaging biological and tissue-equivalent targets with high-energy (800 MeV) proton microscopy are presented here. Although we used a proton microscope setup at ITEP (Moscow, Russia) optimized for fast dynamic experiments in material research, we could reach a spatial resolution of 150 µm with approximately 10(10) protons per image. The potential of obtaining high-resolution online imaging of the target using a therapeutic proton beam in the GeV energy region suggests that high-energy proton microscopy may be used for image-guided proton radiosurgery.

[Perception of short musical fragments by hypomaniac patients].

Emotional assessment of short fragments from pieces of chamber music was studied in patients in steady elated mood (hypomaniac). Six fragments, assessed by healthy subjects as emotionally positive, neutral or negative were used. The examined subjects were shown to be capable for an emotional assessment of the musical fragments. The most pronounced difference of the examined patients from healthy subjects and depressive patients examined earlier was the predominance of neutral assessments in hypomaniacs.