RESUMO
The aim of this work was to examine the content of thioredoxin-reductase in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of thioredoxin-reductase in age-dependent changes in the number of fibroblasts in the dermis. Thioredoxin-reductase, proliferating cells nuclear antigen (PCNA), marker of fibroblasts vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for thioredoxin reductase in the dermis is increased from 20 weeks of pregnancy until 20 years old, is not changed from 21 to 60 years old, and is increased again from 61 to 85 years old. Most expressed age related increase in portion of thioredoxin-reductase positive dermal fibroblasts is present form birth until 20 years as compared to antenatal period. General number and percent of PCNA positive fibroblasts in dermis are decreased with age with more expressed changes until 40 years old. Correlation analysis showed that age dependent decrease in the number of fibroblasts and their proliferative activity is significantly associated with increase in thioredoxin-reductase positive fibroblasts in dermis. Results obtained allow to suggest that thioredoxin-reductase plays a role in age dependent decrease in the number of fibroblasts and their proliferation in human dermis.
Assuntos
Derme , Tiorredoxina Dissulfeto Redutase , Humanos , Feminino , Gravidez , Idoso de 80 Anos ou mais , Idoso , Antígeno Nuclear de Célula em Proliferação , Envelhecimento , Fibroblastos , TiorredoxinasRESUMO
The aim of this work was to examine the content of thioredoxin interacting protein in fibroblasts of human dermis from the development until 85 years old, and defining of a role of thioredoxin interacting protein in age-dependent changes in the number of fibroblasts in the dermis. Thioredoxin interacting protein, proliferating cells nuclear antigen (PCNA), marker of fibroblasts vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for thioredoxin interacting protein in the dermis is increased from 20 weeks of pregnancy until 60 years old followed by a little decrease in age interval 61-85 years old. General number and percent of PCNA positive fibroblasts in dermis are decreased with age with more expressed changes until 40 years old. Correlation analysis showed that age dependent decrease in the number of fibroblasts and their proliferative activity is significantly associated with increase in thioredoxin interacting protein positive fibroblasts in dermis. Results obtained allow to suggest that thioredoxin interacting protein plays a role in age dependent decrease in the number of fibroblasts and their proliferation in human dermis.
Assuntos
Envelhecimento , Derme , Feminino , Gravidez , Humanos , Idoso , Idoso de 80 Anos ou mais , Derme/metabolismo , Antígeno Nuclear de Célula em Proliferação/metabolismo , Envelhecimento/metabolismo , Fibroblastos/metabolismo , Tiorredoxinas/metabolismoRESUMO
The aim of this work was to examine the content of thioredoxin in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of thioredoxin in age-dependent changes in the number of fibroblasts in the dermis. Thioredoxin, proliferating cells nuclear antigen (PCNA), marker of fibroblasts vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for thioredoxin in the dermis is increased from 20 weeks of pregnancy until 85 years old. Most expressed age related increase in portion of thioredoxin positive dermal fibroblasts (more than 9 times) is present form birth until 20 years as compared to antenatal period. General number and percent of PCNA positive fibroblasts in dermis are decreased with age with more expressed changes until 40 years old. Correlation analysis showed that age dependent decrease in the number of fibroblasts and their proliferative activity is significantly associated with increase in thioredoxin positive fibroblasts in dermis. Results allow to suggest that thioredoxin play a role in age dependent decrease in the number of fibroblasts and their proliferation in human dermis.
Assuntos
Derme , Fibroblastos , Idoso de 80 Anos ou mais , Envelhecimento , Feminino , Humanos , Gravidez , Antígeno Nuclear de Célula em Proliferação , Tiorredoxinas , VimentinaRESUMO
The aim of this work was to examine the content of p23 in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of p23 in age-dependent changes in the number and proliferation of fibroblasts in the dermis. p23, proliferating cells nuclear antigen (PCNA), fibroblasts marker vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for p23 in the dermis is gradually increased from 20 weeks of pregnancy until 85 years old. Age-related increase in a portion of fibroblasts with positive staining for p23 is significantly correlated with an age-related decrease in total number and percent of PCNA positive fibroblasts in human dermis. Age-related increase in the content of p23 in fibroblasts is involved in an age-dependent decrease in their total number and proliferation in the dermis.
Assuntos
Derme , Envelhecimento da Pele , Idoso de 80 Anos ou mais , Envelhecimento , Feminino , Fibroblastos , Humanos , Gravidez , Antígeno Nuclear de Célula em ProliferaçãoRESUMO
The aim of this work was to examine the content of aryl hydrocarbon receptor interacting protein (AIP) in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of AIP in age-dependent changes in the number of fibroblasts in the dermis. AIP, proliferating cells nuclear antigen (PCNA) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for AIP in the dermis is gradually increased from 20 weeks of pregnancy until 85 years old. A total number and percent of PCNA positive fibroblasts in dermis decreased with progression of age. Most sufficient age-dependent reduction in a total and PCNA positive number of dermal fibroblast was observed from antenatal until 40 years of life. Correlation analysis showed that both age-dependent decrease in the number of fibroblasts and retardation of their proliferation are significantly associated with age-related increase in the number of AIP positive fibroblasts in dermis. Results allow to suggest that AIP is involved in age-dependent decrease in the number and proliferation of fibroblasts in human dermis.
Assuntos
Derme , Receptores de Hidrocarboneto Arílico , Idoso de 80 Anos ou mais , Envelhecimento , Feminino , Fibroblastos , Humanos , Gravidez , Antígeno Nuclear de Célula em Proliferação , PeleRESUMO
The aim of this work was to examine the content of arylhydrocarbon receptor nuclear translocator (ARNT) in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of ARNT in age-dependent changes in the number of fibroblasts in the dermis. ARNT, proliferating cells nuclear antigen (PCNA) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for ARNT in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of ARNT positive fibroblasts in dermis is increased sufficiently since 41 year old until 60-85 years old group. A total number and percent of PCNA positive fibroblasts in dermis decreased with progression of age. Most sufficient age-dependent reduction in a total and PCNA positive number of dermal fibroblast was observed from antenatal until 40 years of life. Age-related changes in the content of ARNT in fibroblasts is not associated with an age-related decrease in total number and percent of PCNA positive fibroblasts the dermis.
Assuntos
Envelhecimento/metabolismo , Translocador Nuclear Receptor Aril Hidrocarboneto/metabolismo , Envelhecimento da Pele , Pele/metabolismo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Derme/citologia , Derme/metabolismo , Feto/metabolismo , Fibroblastos/metabolismo , Humanos , Lactente , Recém-Nascido , Pele/citologia , Adulto JovemRESUMO
The aim of this work was to examine the content of heat shock protein 90 (HSP90) in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of HSP90 in age-dependent changes in the number of fibroblasts in the dermis. HSP90, proliferating cells nuclear antigen (PCNA) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for HSP90 in the dermis is not changed from 20 weeks of development to 20 years old. Percent of HSP90 positive fibroblasts in dermis is decreased from 21 to 60 years old. From 61 year, the number of HSP90 positive fibroblasts in dermis is increased. Age-related changes in the number of HSP90 positive fibroblasts is not statistically associated with an age-related decrease in a total number and percent of PCNA positive fibroblasts the dermis.
Assuntos
Envelhecimento , Derme/citologia , Fibroblastos/metabolismo , Proteínas de Choque Térmico HSP90/metabolismo , Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Gravidez , Adulto JovemRESUMO
The aim of this work was to examine the content of Yes-associated protein (YAP) in fibroblasts and blood microvessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of YAP in age-dependent changes in the number of fibroblasts and blood microvessels in the dermis. YAP, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for YAP in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of YAP positive fibroblasts in dermis is increased sufficiently since 41 years old until 60-85 years old group. The content of YAP in blood microvessels in the human dermis is decreased sufficiently from 20 weeks of pregnancy until 40 years old. Age-related changes in the content of YAP in fibroblasts and blood microvessels is not statistically associated with an age-related decrease in total number and percent of PCNA positive fibroblasts, the number of blood vessels in the dermis.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Células Endoteliais , Envelhecimento da Pele , Fatores de Transcrição , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Envelhecimento , Derme/citologia , Derme/metabolismo , Feminino , Fibroblastos/metabolismo , Humanos , Pessoa de Meia-Idade , Gravidez , Envelhecimento da Pele/fisiologia , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Proteínas de Sinalização YAPRESUMO
The aim of this work was to examine the content of Piezo1 in fibroblasts and blood vessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of Piezo1 in age-dependent changes in the number of fibroblasts and blood vessels in the dermis. Piezo1, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD31 were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for Piezo1 in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of Piezo1 positive fibroblasts in dermis is increased sufficiently since 41 years old until 60-85 years old group. The content of Piezo1 in blood vessels in the human dermis is decreased sufficiently from 20 weeks of pregnancy until 40 years old. Age-related changes in the content of Piezo1 in fibroblasts and blood vessels is not associated with an age-related decrease in total number and percent of PCNA positive fibroblasts, the number of blood vessels in the dermis.
Assuntos
Vasos Sanguíneos , Derme , Fibroblastos , Canais Iônicos , Envelhecimento da Pele , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Vasos Sanguíneos/fisiologia , Criança , Pré-Escolar , Derme/irrigação sanguínea , Derme/citologia , Derme/embriologia , Derme/crescimento & desenvolvimento , Feminino , Fibroblastos/citologia , Fibroblastos/metabolismo , Humanos , Lactente , Canais Iônicos/metabolismo , Masculino , Pessoa de Meia-Idade , Molécula-1 de Adesão Celular Endotelial a Plaquetas/metabolismo , Gravidez , Antígeno Nuclear de Célula em Proliferação/metabolismo , Envelhecimento da Pele/fisiologiaRESUMO
The aim of this work was to examine the content of transforming growth factor-ß (TGF-ß) in fibroblasts and blood microvessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of TGF-ß in age-dependent changes in the number of fibroblasts and blood microvessels in the dermis. TGF-ß, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD 31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for TGF-ß in the dermis is increased from 20 weeks of pregnancy until 85 years old. More expressed growth in percent of TGF-ß positive fibroblasts in the human dermis is observed after birth and after 40 years old. The content of TGF-ß in blood microvessels in the human dermis is decreased sufficiently from 41 years old. Age-related increase in a portion of fibroblasts with positive staining on TGF-ß is associated with an age-related decrease in total number and percent of PCNA positive fibroblasts in human dermis. Age-related decrease in the content of TGF-ß in blood microvessels is accompanied with an age-related decrease in their number in the dermis. Age-related increase in the content of TGF-ß in fibroblasts is involved in an age-dependent decrease in their total number and proliferation in the dermis. Age-related decrease in the content of TGF-ß in blood microvessels in dermis takes part in the age-related diminishing of blood microvessels number in the dermis.
Assuntos
Envelhecimento , Derme , Pele , Fator de Crescimento Transformador beta , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Fibroblastos , Humanos , Pessoa de Meia-Idade , Gravidez , Pele/metabolismo , Fatores de Crescimento TransformadoresRESUMO
The aim of this work was to examine the content of transcription coactivator with PZD-binding motif (TAZ) in fibroblasts and blood vessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of TAZ in age-dependent changes in the number of fibroblasts and blood vessels in the dermis. TAZ, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for TAZ in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of TAZ positive fibroblasts in dermis is increased since 41 years old until 60-85 years old group. The content of TAZ in blood vessels in the human dermis is decreased sufficiently from 20 weeks of pregnancy until 40 years old followed by an increase from 41 years old. From 61 to 85 years of life, content of TAZ in dermal vessels was not differ from those in 41-60 age group. Age-related changes in the content of TAZ in fibroblasts and blood vessels is not associated with an age-related decrease in total number and percent of PCNA positive fibroblasts, the number of blood vessels in the dermis.
Assuntos
Envelhecimento , Fibroblastos/citologia , Proteínas Serina-Treonina Quinases/genética , Envelhecimento da Pele/genética , Transativadores/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Derme/irrigação sanguínea , Derme/citologia , Feminino , Via de Sinalização Hippo , Humanos , Lactente , Recém-Nascido , Pessoa de Meia-Idade , Gravidez , Transdução de Sinais , Proteínas com Motivo de Ligação a PDZ com Coativador Transcricional , Adulto JovemRESUMO
The goal of this study was to examine the contents of D114 and Jag-1 angiogenesis regulators in human dermis at different age periods. D114 and Jag-1 were demonstrated by indirect immunohistochemistry in skin sections of fetuses of 20-40 gestational weeks and in persons aged from birth to 85 years. D114 was studied in 150 skin samples of 72 females and 78 males, while Jag-1 was examined in 120 samples of 58 females and 62 males. It is found that the immunoreactivity was mainly expressed by the endothelial cells. Vessels, which gave a positive reaction to D114 and Jag-1, were found throughout the entire thickness of the dermis, both in fetuses, and people of all age groups. Expression of D114 in the vessels of dermal microvasculature was shown to increase from 20 weeks of gestation to 20 years. With the further age increase, the intensity of the reaction of blood vessels for D114 was decreased. Expression of Jag-1 in dermal microvessels was enhanced from 20 weeks of gestation to 85 years. The results are discussed in connection with the role of D114 and Jag-1 in angiogenesis in human dermis during ontogeny.
