Aerobic exercise prevents cardiomyocyte damage caused by oxidative stress in early cardiovascular disease by increasing vascularity while L-arginine supplementation prevents it by increasing activation of the enzyme nitric oxide synthase.

L-Arginine and chronic exercise reduce oxidative stress. However, it is unclear how they affect cardiomyocytes during cardiovascular disease (CVD) development. The aim of this research was to investigate the possible effects of L-arginine supplementation and aerobic training on systemic oxidative stress and their consequences on cardiomyocytes during cardiometabolic disease onset caused by excess fructose. Wistar rats were allocated into four groups: control (C), fructose (F, 10% fructose in water), fructose training (FT; moderate running, 50-70% of the maximal velocity), and fructose arginine (FA; 880 mg/kg/day). Fructose was given for two weeks and fructose plus treatments for the subsequent eight weeks. Body composition, blood glucose, insulin, lipid profile, lipid peroxidation, nitrite, metalloproteinase-2 (MMP-2) activity, left ventricle histological changes, microRNA-126, -195, and -146, eNOS, p-eNOS, and TNF-α expressions were analyzed. Higher abdominal fat mass, triacylglycerol level, and insulin level were observed in the F group, and both treatments reversed these alterations. Myocardial vascularization was impaired in fructose-fed groups, except in FT. Cardiomyocyte hypertrophy was observed in all fructose-fed groups. TNF-α levels were higher in fructose-fed groups than in the C group, and p-eNOS levels were higher in the FA than in the C and F groups. Lipid peroxidation was higher in the F group than in the FT and C groups. During CVD onset, moderate aerobic exercise reduced lipid peroxidation, and both training and L-arginine prevented metabolic changes caused by excessive fructose. Myocardial vascularization was impaired by fructose, and cardiomyocyte hypertrophy appeared to be influenced by pro-inflammatory and oxidative environments.

Aerobic exercise prevents cardiomyocyte damage caused by oxidative stress in early cardiovascular disease by increasing vascularity while L-arginine supplementation prevents it by increasing activation of the enzyme nitric oxide synthase

L-Arginine and chronic exercise reduce oxidative stress. However, it is unclear how they affect cardiomyocytes during cardiovascular disease (CVD) development. The aim of this research was to investigate the possible effects of L-arginine supplementation and aerobic training on systemic oxidative stress and their consequences on cardiomyocytes during cardiometabolic disease onset caused by excess fructose. Wistar rats were allocated into four groups: control (C), fructose (F, 10% fructose in water), fructose training (FT; moderate running, 50-70% of the maximal velocity), and fructose arginine (FA; 880 mg/kg/day). Fructose was given for two weeks and fructose plus treatments for the subsequent eight weeks. Body composition, blood glucose, insulin, lipid profile, lipid peroxidation, nitrite, metalloproteinase-2 (MMP-2) activity, left ventricle histological changes, microRNA-126, -195, and -146, eNOS, p-eNOS, and TNF-α expressions were analyzed. Higher abdominal fat mass, triacylglycerol level, and insulin level were observed in the F group, and both treatments reversed these alterations. Myocardial vascularization was impaired in fructose-fed groups, except in FT. Cardiomyocyte hypertrophy was observed in all fructose-fed groups. TNF-α levels were higher in fructose-fed groups than in the C group, and p-eNOS levels were higher in the FA than in the C and F groups. Lipid peroxidation was higher in the F group than in the FT and C groups. During CVD onset, moderate aerobic exercise reduced lipid peroxidation, and both training and L-arginine prevented metabolic changes caused by excessive fructose. Myocardial vascularization was impaired by fructose, and cardiomyocyte hypertrophy appeared to be influenced by pro-inflammatory and oxidative environments.

Ineficacy of portable device AccutrendⓇ Plus in equine internal medicine / Ineficácia do aparelho portátil AccutrendⓇ Plus na clínica médica de equinos

Four blood components (glucose, lactate, triglycerides and cholesterol) were determined and compared using a portable device (Accutrend® Plus, ROCHE) and laboratory methods. Blood samples from 20 horses were analyzed (11 geldings and 9 mares, from various breeds with age and weight varying between 8 ± 5 years and 327.60 ± 64.02 Kg, respectively. After complete clinical examination, blood was withdrawn through venipunction of the external jugular and these samples were analyzed using a portable device and compared to laboratory results. Statistical analyses were used to compare both methodologies. The results showed that glucose data were not different with a weak Pearson's correlation and no agreement according to Bland-Altman method. The results obtained for lactate according to both methods were also not in agreement. Triglycerides and cholesterol data could not be compared due to the fact that reference values for resting horses lie below the reading range of the portable device. The present study showed that the use of AccutrendⓇPlus to determine glucose, lactate, triglycerides, and cholesterol levels, is not adequate in equine internal medicine.

Comparou-se nesse estudo a determinação de quatro constituintes sanguíneos (glicose, lactato, triglicérides e colesterol), entre um aparelho portátil e métodos laboratoriais convencionais. Foram analisados o sangue de 20 equinos (11 machos e 9 fêmeas), de diversas raças com idades variando entre 8 ± 5 anos e peso corpóreo de 327,60 ± 64,02 Kg. Após exame clínico completo de cada animal realizou-se punção da veia jugular externa para coleta de amostra sanguínea, de maneira que a mesma amostra, foi utilizada para determinação no aparelho portátil e no laboratório. Na comparação entre as metodologias, em relação a glicose, não foi notada diferença entre as médias (teste T de Student), a correlação de Pearson revelou-se fraca e não houve concordância segundo o método de Bland-Altman. Para o lactato também não se observou concordância entre os dois métodos. Quanto aos triglicérides e colesterol foi possível apenas a análise descritiva dos resultados, devido as concentrações dos padrões fisiológicos de equinos em repouso, se apresentarem inferiores ao intervalo de leitura do aparelho portátil. Este trabalho demonstrou que o uso do referido aparelho para determinação de glicose, lactato, triglicerides e colesterol não é adequado na clínica médica equina.

Arthroscopic exposure of the patellar articular surface.

Patellofemoral cartilage degeneration is usually associated with a tight lateral retinaculum (lateral hyperpressure). The position of the patella on the femoral groove under hyperpressure makes reasonable access to the cartilage defects difficult for the arthroscopist. Frequently, the surgeon can barely see the cartilage, and the problem cannot be satisfactorily managed. By performing a lateral release and tilting the patella with a laterally drilled Kirschner wire, the articular surface of the patella can be completely exposed, allowing even perpendicular holes to be drilled whenever necessary.

Medial patellofemoral ligament reconstruction for recurrent dislocation of the patella: a preliminary report.

We present a surgical technique for the treatment of recurring dislocation of the patella. The procedure, based on Galleazzi's tenodesis of the semitendinosus, uses minimal dissection and small incisions to improve cosmetic results and speed recovery. A polyester ligament is passed through a bone tunnel at the midline of the patella and fixed at the medial condyle, thus creating a medial patellofemoral neoligament. We have reviewed the results of 30 knees in 30 patients with an average follow-up of 39 months. Twenty-five patients (83.3%) showed significant improvement of their initial complaints.

Introducao a cirurgia artroscopica. / An introduction to arthroscopic surgery

A cirurgia astroscopica e apresentada e discutida atraves da analise de procedimentos basico. As indicacoes, vantagens e desvantagens da tecnica foram examinadas de forma a oferecer embasamento pratico-teorico aqueles que estao iniciando na astroscopia. Verificou-se, alem disso, que a cirurgia artroscopica proporcionou, aos pacientes, uma reabilitacao mais rapida do que a cirurgia convencional. Nao se pode, no entanto, afirmar que sua capacidade curativa seja maior do que a da cirurgia convencional, mas sim que se chegou ao mesmo objetivo por um caminho curto e menos arduo

Reconstrucao dos ligamentos cruzados do joelho com o tendao duplo do semitendinoso. / Reconstruction of the cruciate ligament with looped free tendon of the semitendinous muscle

A reconstrucao dos ligamentos cruzados do joelho com o tendao duplo do semitendinoso e apresentada e discutida com base nos resultados obtidos em 21 pacientes. Para a fixacao do tendao duplo na tibia e femur, utilizamos um tarugo de osso esponjoso. Esse tarugo foi obtido com a trefina que simultaneamente fez os tuneis de ancoragem na tibia e femur. Dezessete pacientes submeteram-se a reconstrucao do ligamento cruzado anterior e quatro do posterior. A manobra do ressalto lateral, o teste de Lachmann e gaveta posterior e anterior foram verificados no pre e pos-operatorio. Comparando estes achados, obtivemos 86,3% de bons resultados e 13,7% de regulares nas reconstrucoes do ligamento cruzado anterior. Quanto ao ligamento cruzado posterior, ficamos com 75% de bons e 25% de regulares.No entanto, nossa pequena casuistica com uma evolucao media de 13 meses ainda nao permite conclusoes definitivas