RESUMO
En la práctica diaria, cuando se realiza la determinación de creatinquinasa isoenzima MB (CK-MB) por el método inmunológico, es habitual obtener resultados incongruentes: valores de CK-MB cercanos o superiores al de creatinquinasa total (CK). En el presente trabajo se informa acerca de las interferencias más frecuentes encontradas con este método en nuestro Instituto
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Creatina Quinase/sangue , Testes Imunológicos/métodos , Creatina Quinase , Creatina Quinase/sangue , Creatina Quinase/imunologia , Creatina Quinase/isolamento & purificação , Eletroforese em Acetato de Celulose/normas , Eletroforese em Acetato de Celulose/estatística & dados numéricosRESUMO
En la práctica diaria, cuando se realiza la determinación de creatinquinasa isoenzima MB (CK-MB) por el método inmunológico, es habitual obtener resultados incongruentes: valores de CK-MB cercanos o superiores al de creatinquinasa total (CK). En el presente trabajo se informa acerca de las interferencias más frecuentes encontradas con este método en nuestro Instituto (AU)
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Creatina Quinase/sangue , Testes Imunológicos/métodos , Creatina Quinase/imunologia , Eletroforese em Acetato de Celulose/normas , Eletroforese em Acetato de Celulose/estatística & dados numéricos , Creatina Quinase/isolamento & purificação , Creatina Quinase/sangue , Creatina Quinase/diagnósticoRESUMO
The oxidative stress in human erythrocytes was studied in asymptomatic and symptomatic patients infected by the human immunodeficiency virus (HIV), and patients with the acquired immunodeficiency syndrome (AIDS). tert-Butyl hydroperoxide initiated chemiluminescence, superoxide dismutase and catalase activities, and total glutathione were evaluated in the erythrocytes and the total antioxidant capacity in the plasma of control, patients infected with HIV that have not yet developed acquired immunodeficiency syndrome, and patients in the later stage of AIDS. tert-Butyl hydroperoxide initiated chemiluminescence was increased by 33% in asymptomatic (stage A1) and symptomatic patients (stage B2) infected with HIV and 82% for patients with AIDS (stage B3) (P < 0.05). While catalase activity did not show any difference between patients and controls, other indices showed differences that, in some cases, reached statistical significance. Superoxide dismutase activity was increased by 24% in stages A1 and B2 of HIV infection and 65% in patients in stage B3 (P < 0.05). Glutathione was decreased by 20% in stages A1 and B2, and by 32% in stage B3 patients (P < 0.05). Total plasma antioxidant capacity was increased in 30 and 57% for the asymptomatic and AIDS patients groups, respectively (P < 0.05). The data indicate that erythrocyte's oxidative stress is associated with the progressive development of HIV disease. Parameters indicating oxidative stress could be an interesting form to screen the evolution of these patients and their response to anti-oxidant therapies.
Assuntos
Eritrócitos/metabolismo , Infecções por HIV/metabolismo , Estresse Oxidativo/fisiologia , Adulto , Antioxidantes/metabolismo , Catalase/sangue , Catalase/metabolismo , Feminino , Radicais Livres/metabolismo , Glutationa/sangue , Glutationa/metabolismo , Humanos , Medições Luminescentes , Masculino , Peróxidos/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/sangue , Superóxido Dismutase/metabolismo , terc-Butil HidroperóxidoRESUMO
HTLV-I and HTLV-II are two related retroviruses that are transmitted by sexual contact, breast feeding, blood transfusion and needle sharing. In this study the prevalence of HTLV-I and HTLV-II was evaluated in voluntary blood donors as a measure of the infection in the general population. Samples were tested by a gelatine particle agglutination test and repeatedly reactive samples were confirmed by Western blot tests (WBT), enriched with recombinant rgp21, rgp461 y rgp4611 proteins, which differentiates HTLV-I and HTLV-II antibodies. Of 19,426 samples, 40 were repeatedly reactive by particle agglutination (0.21%). When analyzed by WBT, 6 met the criteria for HTLV-I (0.036%), 2 for HTLV-II (0.01%) and 1 for HTLV-I/II, 13 samples were indeterminate and 18 were negative. The prevalence is low and comparable to that from non endemic countries. Screening for anti HTLV-I/II antibodies is necessary to prevent transmission through blood transfusions.
Assuntos
Infecções por HTLV-I/transmissão , Infecções por HTLV-II/transmissão , Adolescente , Adulto , Idoso , Testes de Aglutinação/métodos , Argentina , Doadores de Sangue , Western Blotting , Feminino , Anticorpos Anti-HTLV-I/análise , Anticorpos Anti-HTLV-II/análise , Vírus Linfotrópico T Tipo 1 Humano/imunologia , Vírus Linfotrópico T Tipo 2 Humano/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , PrevalênciaRESUMO
HTLV-I and HTLV-II are two related retroviruses that are transmitted by sexual contact, breast feeding, blood transfusion and needle sharing. In this study the prevalence of HTLV-I and HTLV-II was evaluated in voluntary blood donors as a measure of the infection in the general population. Samples were tested by a gelatine particle agglutination test and repeatedly reactive samples were confirmed by Western blot tests (WBT), enriched with recombinant rgp21, rgp461 y rgp4611 proteins, which differentiates HTLV-I and HTLV-II antibodies. Of 19,426 samples, 40 were repeatedly reactive by particle agglutination (0.21
). When analyzed by WBT, 6 met the criteria for HTLV-I (0.036
), 2 for HTLV-II (0.01
) and 1 for HTLV-I/II, 13 samples were indeterminate and 18 were negative. The prevalence is low and comparable to that from non endemic countries. Screening for anti HTLV-I/II antibodies is necessary to prevent transmission through blood transfusions.
