RESUMO
1. To clarify the possibility that the metabolism of 26,27-hexafluoro-1 alpha,25-dihydroxyvitamin D3 [F6-1,25(OH)2D3] to 26,27-hexafluoro-1 alpha,23(S),25-trihydroxyvitamin D3 [F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to 26,27-hexafluoro-23-oxo-1 alpha,25-dihydroxyvitamin D3 [F6-23-oxo-1,25(OH)2D3] are catalysed by 25-hydroxyvitamin D3 24-hydroxylase (CYP24), ROS17/2.8 cells transfected with a plasmid expressing CYP24 [pSVL-CYP24(+)] and a corresponding blank plasmid [pSLV-CYP24R(-)] were used. 2. Incubation of [1 beta-3H]-F6-1,25(OH)2D3 for 2 and 5 days with ROS17/2.8 cells transfected with pSVL-CYP24(+) generated a metabolite that co-migrated with authentic F6-1,23,25(OH)3D3 in both normal phase and reversed-phase HPLC systems. 3. Incubation of [1 beta-3H]-F6-1,23,25(OH)3D3 for 5 days with pSVL-CYP24(+)- transfected ROS 17/2.8 cells generated a metabolite that co-migrated with authentic F6-23-oxo-1,25(OH)2D3. In contrast, the metabolites F6-1,23,25(OH)3D3 or F6-23-oxo-1,25(OH)2D3 were not generated in the cells transfected with pSVL-CYP24R(-). 4. The results indicate that CYP24 catalyses the conversion of F6-1,25(OH)2D3 to F6-1,23,25(OH)3D3 and that of F6-1,23,25(OH)3D3 to F6-23-oxo-1,25(OH)2D3.
Assuntos
Calcitriol/análogos & derivados , Calcitriol/metabolismo , Sistema Enzimático do Citocromo P-450/biossíntese , Sistema Enzimático do Citocromo P-450/fisiologia , Esteroide Hidroxilases/biossíntese , Esteroide Hidroxilases/fisiologia , Animais , Linhagem Celular , Cromatografia Líquida de Alta Pressão , Modelos Químicos , Osteoblastos/metabolismo , Plasmídeos/metabolismo , Ratos , Fatores de Tempo , Transfecção , Células Tumorais Cultivadas , Vitamina D3 24-HidroxilaseRESUMO
A technique for the removal of cemented post-cores by means of ultrasonic vibration was studied clinically and histologically. The removal time was measured for 80 extracted human teeth, including 40 single-rooted teeth and 40 multiple rooted ones and then statistically analyzed. The periapical tissue reaction to the ultrasonic vibration was also examined histologically in dogs. The best result was achieved when lateral vibration was simultaneously given by 2 removal tips using 2 ultrasonic generators. There was little harmful influence on the periodontal tissue, excluding a transient reversible reaction.
Assuntos
Descolagem Dentária/métodos , Técnica para Retentor Intrarradicular/instrumentação , Ultrassom , Animais , Cães , Humanos , Tecido Periapical/fisiologia , Retratamento , Fatores de Tempo , VibraçãoRESUMO
The effect of eliminating the smear layer by means of 15% EDTA solution as a root canal irrigant was studied in 189 single-rooted infected teeth. Each tooth was treated at two appointments, and the root canal bacteriological examination was studied on the first (pretreatment, and after enlargement and irrigation) and second (pretreatment) visits. The root canals were irrigated with 15% EDTA solution with ultrasonics agitation. No antibacterial intracanal medications were used between the appointment. When 15% EDTA solution was used, no bacteria could be recovered from 93 of 129 root canals at the sampling stage on second visit. No bacteria were found in 21 of 60 root canals when saline solution was used as an irrigant. These results suggest that 15% EDTA solution is more effective than saline solution as a root canal irrigant.
