RESUMO
OBJECTIVE: Chronic inflammation has been recognized as having a prominent role pathogenesis of benign prostatic hyperplasia (BPH) and cancer. It is believed that chronic inflammation induces prostatic fibromuscular growth. This correlation has been clearly illustrated by both in vivo and in vitro studies; however, current experimental models of BPH require complex surgery or hormonal treatment. Therefore, the aim of the present study was to propose a new murine model of BPH/prostatitis induced by intraurethral injection of LPS. METHODS: Male Swiss and C57Bl/6 mice were then sacrificed 3, 7, 10, and 14 days after intraurethral injection of LPS. The prostates were quickly dissected and fixed for morphological and immunohistochemical analyses. RESULTS: The results showed that LPS played an important role in the cell proliferation of the prostate. Histological and ultrastructural analysis showed epithelial hyperplasia, clear stromal cells, little inflammatory infiltration, and heavy bleeding. Treatment with LPS also promoted the increase of growth factor (FGF-7 and TGF-ß), α-actin, and proinflammatory cytokines (IL-1, IL-6, IL-17), both in the stroma and epithelium. CONCLUSION: According to the present findings, it can be concluded that the intraurethral administration of LPS promotes tissue remodeling, as well as stimulating the pattern of pro-inflammatory cytokines, and therefore, constitutes an effective experimental model of BPH/inflammation.
Assuntos
Inflamação/induzido quimicamente , Lipopolissacarídeos/toxicidade , Próstata/efeitos dos fármacos , Hiperplasia Prostática/induzido quimicamente , Animais , Citocinas/imunologia , Modelos Animais de Doenças , Fator 7 de Crescimento de Fibroblastos/imunologia , Inflamação/imunologia , Inflamação/patologia , Injeções , Masculino , Camundongos Endogâmicos C57BL , Próstata/imunologia , Próstata/patologia , Hiperplasia Prostática/imunologia , Hiperplasia Prostática/patologia , UretraRESUMO
Neste Relato de Pesquisa, a autora trabalha a questão da estereotipia da relação entre profissionais e paciente no âmbito de uma instituição psiquiátrica. Utiliza como principal suporte, na fundamentação teórica, os aportes de Enrique Pichón RIVIÈRE (1970). A pesquisa foi realizada entre 1981 e 1983 no Paraná e fez parte de sua dissertação de Mestrado defendida em 1989 na Pontifícia Universidade Católica de São Paulo (AU)
