RESUMO
Tongue squamous cell carcinoma (TSCC) is one of the most common types of oral cancer; however, its molecular mechanisms remain unclear. In this study, methylated DNA immunoprecipitation (MeDIP) coupled with methylation microarray analysis was performed to screen for aberrantly methylated genes in adjacent normal control and TSCC tissues from 9 patients. Roche NimbleGen Human DNA Methylation 385K Promoter Plus CpG Island Arrays were used to detect 28,226 CpG sites. A total of 1,269 hypermethylated CpG sites covering 330 genes and 1,385 hypomethylated CpG sites covering 321 genes were found in TSCC tissue, compared to the adjacent normal tissue. Furthermore, we chose three candidate genes (FBLN1, ITIH5 and RUNX3) and validated the DNA methylation status by methylation-specific PCR (MS-PCR) and the mRNA expression levels by reverse transcription PCR (RT-PCR). In TSCC tissue, FBLN1 and ITIH5 were shown to be hypermethylated and their expression was found to be decreased, and RUNX3 was shown to be hypomethylated, however, its mRNA expression was found to be increased. In addition, another three genes (BCL2L14, CDCP1 and DIRAS3) were tested by RT-PCR. In TSCC tissue, BCL2L14 and CDCP1 expressions were markedly upregulated, and DIRAS3 expression was significantly downregulated. Our data demonstrated that aberrant DNA methylation is observed in TSCC tissue and plays an important role in the tumorigenesis, development and progression of TSCC.
Assuntos
Carcinoma de Células Escamosas/genética , Metilação de DNA , Neoplasias da Língua/genética , Antígenos CD/genética , Antígenos de Neoplasias , Proteínas de Ligação ao Cálcio/genética , Carcinogênese/genética , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Moléculas de Adesão Celular/genética , Subunidade alfa 3 de Fator de Ligação ao Core/genética , Ilhas de CpG , Progressão da Doença , Estudo de Associação Genômica Ampla/métodos , Humanos , Proteínas de Neoplasias/genética , Proteínas Secretadas Inibidoras de Proteinases/genética , RNA Mensageiro/genética , Neoplasias da Língua/metabolismo , Neoplasias da Língua/patologia , Regulação para Cima , Proteína bcl-X/genética , Proteínas rho de Ligação ao GTP/genéticaRESUMO
OBJECTIVE: To analyze the possibility and practicality of using free anterolateral thigh flap to repair defects of oromaxillo-facial region. METHODS: One hundred and seventy-two patients underwent anterolateral thigh flap (ALP) reconstruction from Jan. 2007 to Dec. 2008. The size of the defect ranges from 4 cm x 4 cm to 10 cm x 25 cm. One hundred and six flaps were used for tongue reconstruction, 14 flaps for buccal reconstruction, 15 flaps for mouth floor reconstruction, 1 flap for lower lip and mental region reconstruction, 6 flaps for hard and soft palate reconstruction, 3 flap for maxillary part reconstruction, 18 flaps for submaxilla part reconstruction, 2 flaps for pharynx oralis reconstruction, 3 flaps for repairing large-area defect of facial, 1 flap for repairing neck and shoulder region and 3 flaps for repairing defect of Infratemporal fossa. Of these flaps, 151 were musculocutaneous flaps, 13 were fasciocutaneous flaps and 8 were chimeric flaps. RESULTS: The success rate reaches 98.3% (169 of 172). Postoperatively, 4 cases encountered vascular crisis, 1 of which survived completely with successful vessel exploration, 3 cases with partial necrosis; and 3 cases with complete necrosis. One case with bleeding after operation. Six cases with parotid fluidly and parotid fistula, 8 cases with mouth floor fistula, 6 cases with infection, 3 cases with infection and partial necrosis on the lateral thigh. All patients achieved acceptable contour, appearance and function. CONCLUSION: Free anterolateral thigh flap can repair kinds of complicated defects on oromaxillo-facial region.
Assuntos
Fêmur/cirurgia , Procedimentos Cirúrgicos Bucais/métodos , Transplante de Pele/métodos , Retalhos Cirúrgicos , Adolescente , Adulto , Idoso , Face/cirurgia , Feminino , Humanos , Lábio/cirurgia , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/cirurgia , Procedimentos de Cirurgia Plástica/métodos , Coxa da Perna/cirurgia , Transplante Autólogo , Adulto JovemRESUMO
OBJECTIVE: To report the application of the chimeric perforator flap pedicled with descending branch of lateral circumflex femoral artery for large and complicated oromaxillary soft tissue defect. METHODS: Based on the anatomic study of descending branches and cutaneous perforators of lateral circumflex femoral artery, the perforator vessels were found and used as flap pedicle. The perforator flap was made as chimeric flap for repairing the oromaxillary soft tissue defect in 8 cases. The chimeric perforator flaps were divided into three types as anterolateral thigh flaps and anteromedial thigh flaps, anterolateral thigh flaps and rectus femoris perforator flaps, and anterolateral thigh flaps and anterolateral thigh flaps. RESULTS: All the 16 flaps in 8 cases survived completely with no complication. The wounds in donor sites were all primarily closed with no skin graft. The patients were followed up for 1-9 months with good functional and esthetic results. There was no morbidity in donor sites. CONCLUSIONS: The chimeric perforator flap has a large tissue volume for large and complicated oromaxillary defect. There is no need for extra donor site and extra blood vessel anastomosis.