RESUMO
Objective: To establish a method of simultaneous determination of chlorogenic acid,galuteolin,quercetin and acacetin in Chrysanthemi Flos by quantitative analysis of multi-components by single marker( QAMS),and determine the application value of QAMS in the quality control of Chrysanthemi Flos. Methods: The analysis was established to use the Shim-pack C18( 250 mm × 4. 6 mm,5 µm),the mobile phase of water contained acetonitrile( A)-0. 1% phosphoric acid aqueous solution( B) in a gradient elution manner,the flow rate was 1. 0 m L / min,the column temperature was 30 â,the injection volume was 10 µL,the detection wavelength was set at348 nm. A characteristic spectrum was used for identification of four components. By using chlorogenic acid as reference,the relative corrective factors( RCF) of the other three components with reference to chlorogenic acid were calculated. The method was evaluated by comparison of the quantitative results through external standard method and QAMS method. Results: The RCF of galuteolin, quercetin and acacetin with reference to chlorogenic acid were 0. 6865,0. 9976 and 0. 6665,and the RSD( n = 6) were 0. 1439%,0. 2512%,0. 3971%,respectively. The results from QAMS method were not significantly different from those from external standard method. Conclusion: The method with a single marker is accurate and feasible to evaluate the quality of galuteolin,quercetin and acacetin as reference to chlorogenic acid in Chrysanthemi Flos by QAMS.
