RESUMO
IMPORTANCE: Reactive oxygen species (ROS), including superoxide anion, is a series of substances that cause oxidative stress for all organisms. Marine group II (MGII) archaea are mainly live in the surface seawater and exposed to considerable ROS. Therefore, it is important to understand the antioxidant capacity of MGII. Our research found that Fe/Mn- superoxide dismutase (Fe/MnSOD) may be more suitable for MGII to resist oxidative damage, and the changes in oxygen concentrations and SOD metallic cofactors play an important role in the selection of SOD by the 17 clades of MGII, which in turn affects the species differentiation of MGII. Overall, this study provides insight into the co-evolutionary history of these uncultivated marine archaea with the earth system.
Assuntos
Archaea , Oxigênio , Archaea/genética , Espécies Reativas de Oxigênio , Superóxido Dismutase/genética , SuperóxidosRESUMO
The first complete mitochondrial genome sequence of Metapenaeus was characterized from sand shrimp Metapenaeus ensis using shotgun assembly method. The complete mitochondrial DNA sequence is a 15 944 bp circular molecule, and contains 13 protein-coding genes, 22 transfer RNA genes, two rRNA genes, and a control region. The gene arrangements are consistent with the pancrustacean ground pattern. The phylogenetic trees were divided two clades besides outgroup, which belonged to Penaeus s.l. and Metapenaeus genus. The molecular analyses provided robust evidence for the monophyly of Fenneropenaeus, but Litopenaeus is not monophyletic.
Assuntos
Decápodes/genética , Genoma Mitocondrial , Filogenia , Animais , Decápodes/classificação , Região de Controle de Locus Gênico , Fases de Leitura Aberta , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
The complete mitochondrial genome sequence of Fenneropenaeus merguiensis was determined by shotgun assembly method. The complete mitochondrial DNA sequence is a circular molecule with 16,023 bp in length including 13 protein-coding genes, 22 transfer RNA genes, 2 rRNA genes and a control region. The gene arrangements are consistent with the pan crustacean ground pattern. The molecular analyses provided robust evidence for the monophyly of Fenneropenaeus, but Litopenaeus was not monophyletic. Phylogenetic analyses robustly supported the fact that genus Penaeus s.l. contains the two lineages: Marsupenaeus and Penaeus s.s+ Fenneropenaeus + Litopenaeus + Farfantepenaeus.
Assuntos
Genoma Mitocondrial/genética , Penaeidae/genética , Animais , DNA Mitocondrial/genética , Penaeidae/classificação , RNA Ribossômico/genética , RNA de Transferência/genética , Análise de Sequência de DNARESUMO
The complete mitochondrial genome is of great importance for better understanding of the genome-level characteristics and phylogenetic relationships among related species. In this study, Fenneropenaeus penicillatus mitochondrial genome sequence was determined by next-generation sequencing. The complete genome DNA was 16,040 bp in length and consisted of a typical set of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and a putative control region (CR). The gene arrangement is identical to the pancrustacean pattern. The overall base composition of its mitochondrial genome is estimated to be 34.1% for A, 34.1% for T, 12.5% for G and 19.3% for C with a high A+T content (68.2%). The analysis of the average Ka/Ks in the 13 mitochondrial protein-coding genes of penaeid shrimps indicated a strong purifying selection within this group. The phylogenetic analysis based on mitochondrial sequences and 13 concatenated protein-coding genes showed strong statistic support for the following relationship among the five genera ((Penaeus s.s+Fenneropenaeus)+(Litopenaeus+Farfantepenaeus))+Marsupenaeus. The sequence data of F. penicillatus can provide useful information for the studies on molecular systematics, population structure, stock evaluation and conservation genetics.
