RESUMO
The taxonomic status of a carbendazim-degrading strain, mbc-2(T), isolated from soil under the long-term application of carbendazim in China was determined by means of a polyphasic study. The cells were Gram-stain-positive, motile and rod-shaped. Strain mbc-2(T) grew optimally at pH 7.0, 30-35 °C and in the presence of 1% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain mbc-2(T) fell within the genus Nocardioides, forming a coherent cluster with the type strain of Nocardioides hankookensis, with which it exhibited 16S rRNA gene sequence similarity values of 97.9%. The chemotaxonomic properties of strain mbc-2(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-2,6-diaminopimelic acid, the predominant menaquinone was MK-8 (H4) and the major fatty acid was iso-C(16â:â0). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, unknown phospholipids and an unknown aminolipid. The DNA G+C content was 72 mol%. Strain mbc-2(T) exhibited DNA-DNA relatedness values of 12.5±1.5%, 23.7±2.7% and 26.3±3.2% with respect to Nocardioides hankookensis DS-30(T), Nocardioides aquiterrae GW-9(T) and Nocardioides pyridinolyticus OS4(T). On the basis of the data obtained, strain mbc-2(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides soli sp. nov. is proposed. The type strain is mbc-2(T) (â=âKACC 17152(T)â=âCCTCC AB 2012934(T)).