CLEAR Strategy Inhibited HSV Proliferation Using Viral Vectors Delivered CRISPR-Cas9.

Herpes simplex virus type 1 (HSV-1) is a leading cause of encephalitis and infectious blindness. The commonly used clinical therapeutic drugs are nucleoside analogues such as acyclovir. However, current drugs for HSV cannot eliminate the latent virus or viral reactivation. Therefore, the development of new treatment strategies against latent HSV has become an urgent need. To comprehensively suppress the proliferation of HSV, we designed the CLEAR strategy (coordinated lifecycle elimination against viral replication). VP16, ICP27, ICP4, and gD-which are crucial genes that perform significant functions in different stages of the HSV infection lifecycle-were selected as targeting sites based on CRISPR-Cas9 editing system. In vitro and in vivo investigations revealed that genome editing by VP16, ICP27, ICP4 or gD single gene targeting could effectively inhibit HSV replication. Moreover, the combined administration method (termed "Cocktail") showed superior effects compared to single gene editing, which resulted in the greatest decrease in viral proliferation. Lentivirus-delivered CRISPR-Cas9/gRNA editing could effectively block HSV replication. The CLEAR strategy may provide new insights into the potential treatment of refractory HSV-1-associated diseases, particularly when conventional approaches have encountered resistance.

Efficient monocular vision method used for measuring the angular rate and acceleration in rotation motion.

The rotation motion is one of the most basic dynamic units, whose accurate measure-ment is essential to the aspects of inertial navigation, mechanical manufacturing, robot control. Currently, the laser interferometry (LI), the sensor-based and autocollimator-based methods are commonly applied to the rotation motion measurement. However, they are always difficult to meet the requirements of high-accuracy measurement and wide frequency range. In this study, a novel monocular vision-based measurement method is investigated, which determines the angular rate and angular acceleration by using the improved line segmentation detector (LSD) method with sub-pixel accuracy and simultaneously improves the measurement accuracy and frequency range. Additionally, its hardware implementation only requires a simple and flexible vision measurement system. Several comparison experiments with the LI and grating sensor-based method demonstrate that the investigated method can obtain the measurement resolution of 0.0005° and accuracy of 0.480% in the range from 0.001 to 10 Hz.