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1.
PeerJ ; 12: e17819, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39099661

RESUMO

Background: Isometric training is used in sport, conventional physical activity and rehabilitation. Understandably, there is a great deal of research related to its effect on performance. It is known that the length of the muscle at the moment of contraction is a determinant of strength levels. In the literature we find research on isometric training in short muscle lengths, although it has not been studied in maximally shortened positions or the acute effects that occur after its application. Ballistic stretching (BS) is also popular in sport. Their execution involves actively reaching maximally shortened muscle positions. So far, isometric training has not been compared with protocols involving ballistic stretching. Considering the above, the aim of the present study was to investigate the effects of BS and voluntary isometric contraction at maximal shortening (VICAMS) on range of motion, strength and vertical jump. Methods: The study involved 60 healthy, physically active individuals (40 and 52 years old) who were randomly assigned to three groups: BS, VICAMS and a control group (CG). To assess acute effects, before and after the intervention, active range of motion (AROM), maximal voluntary isometric force (MVIF) and countermovement jump height (CMJ) were determined. Results: Time main effects and time*group interactions were found for all variables (p < 0.001). Between-group differences were shown for the VICAMS group after the intervention, with statistically significant higher AROM values compared to the other groups. MVIF values were also higher in the VICAMS group. Intra-group differences were observed for the VICAMS and Ballistic groups, as values on all variables increased from baseline. For the CMJ, intra-group differences showed that both the VICAMS and BS groups improved values compared to baseline values. Conclusions: The application of VICAMS induced acute improvements over BS in AROM, MVIF and CMJ. These results are important for coaches seeking immediate performance improvement and offer an optimal solution to the warm-up protocol.


Assuntos
Contração Isométrica , Força Muscular , Exercícios de Alongamento Muscular , Amplitude de Movimento Articular , Humanos , Contração Isométrica/fisiologia , Exercícios de Alongamento Muscular/fisiologia , Masculino , Adulto , Força Muscular/fisiologia , Amplitude de Movimento Articular/fisiologia , Pessoa de Meia-Idade , Músculo Esquelético/fisiologia , Feminino
2.
Front Sports Act Living ; 5: 1267593, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38022781

RESUMO

The aim of the present study was to investigate the effect of an incremental running exercise until exhaustion on twitch responses and jump capacity in endurance trained runners. For this purpose, 8 experienced endurance male runners were required to perform neuromuscular function tests before and after a submaximal running bout (control condition -CTR-) or an incremental running test to volitional exhaustion (experimental conditions -EXP-). The twitch interpolation technique was used to assess voluntary activation and muscle contractile properties before and after each condition (CTR and EXP). Countermovement jump was also used to assess the stretch-shortening cycle function before and after both conditions. In addition, rating of perceived exertion, heart rate, blood lactate and skin temperature were also recorded. Only EXP improved jump performance, however, it was also accompanied by a reduction in maximal voluntary contraction and the peak twitch force of the knee extensors evoked by electrical stimulation at 10 Hz (Db10). It is likely that reductions in maximal voluntary contraction may be related to an excitation-contraction coupling failure (i.e. low-frequency fatigue) as suggest the reduction in the Db10. The current results confirm that acute changes in jump performance may not be appropriate to evaluate acute fatigue in endurance trained runners.

3.
J Strength Cond Res ; 37(5): 980-986, 2023 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-36730585

RESUMO

ABSTRACT: Janicijevic, D, González-Hernández, JM, Jiménez-Reyes, P, Márquez, G, and García-Ramos, A. Longitudinal effects of traditional and rest redistribution set configurations on explosive-strength and strength-endurance manifestations. J Strength Cond Res 37(5): 980-986, 2023-This study aimed to compare the long-term effects of resistance training programs based on traditional and rest redistribution set configurations on explosive-strength and strength-endurance performance of lower-body and upper-body muscles. Thirty physically active men were randomly assigned to a traditional group (TRG: 6 sets of 5 repetitions with 3 minutes of interset rest) or a rest redistribution group (RRG: 30 sets of 1 repetition with 31 seconds of interrepetition rest). The training program lasted 6 weeks (2 sessions·wk -1 ), and in each training session, the squat and bench press exercises were performed with maximal concentric effort against approximately the 75% of the 1 repetition maximum. Before and after training, explosive-strength performance (peak velocity reached at submaximal loads during the countermovement jump and bench press throw) and strength-endurance performance (mean set velocity of 10 repetitions using both traditional and cluster sets in the squat and bench press) were assessed. Significant improvements in all dependent variables were observed after training for both the TRG ( p ≤ 0.004; effect size [ES] = 0.63-3.06) and RRG ( p ≤ 0.001; ES = 0.58-3.23). The magnitude of the changes was comparable for both groups with the only exception of the larger improvements observed in the RRG for the bench press mean set velocity using both traditional (ES = 0.77) and cluster (ES = 0.82) set configurations. Traditional and rest redistribution set configurations are equally effective to improve lower-body explosive strength, lower-body strength endurance, and upper-body explosive strength, whereas rest redistribution set configurations could induce greater adaptations in upper-body strength endurance.


Assuntos
Músculo Esquelético , Treinamento Resistido , Masculino , Humanos , Músculo Esquelético/fisiologia , Exercício Físico/fisiologia , Descanso/fisiologia , Adaptação Fisiológica , Força Muscular , Levantamento de Peso/fisiologia
4.
Sensors (Basel) ; 23(2)2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36679451

RESUMO

Introduction: The implementation of optimal sprint training volume is a relevant component of team sport performance. This study aimed to compare the efficiency and effectiveness of two different configurations of within-season training load distribution on sprint performance over 6 weeks. Methods: Twenty male professional FH players participated in the study. Players were conveniently assigned to two groups: the experimental group (MG; n = 11; applying the microdosing training methodology) and the control group (TG; n = 9; traditional training, with players being selected by the national team). Sprint performance was evaluated through 20 m sprint time (T20) m and horizontal force−velocity profile (HFVP) tests before (Pre) and after (Post) intervention. Both measurements were separated by a period of 6 weeks. The specific sprint training program was performed for each group (for vs. two weekly sessions for MG and TG, respectively) attempting to influence the full spectrum of the F-V relationship. Results: Conditional demands analysis (matches and training sessions) showed no significant differences between the groups during the intervention period (p > 0.05). No significant between-group differences were found at Pre or Post for any sprint-related performance (p > 0.05). Nevertheless, intra-group analysis revealed significant differences in F0, Pmax, RFmean at 10 m and every achieved time for distances ranging from 5 to 25 m for MG (p < 0.05). Such changes in mechanical capabilities and sprint performance were characterized by an increase in stride length and a decrease in stride frequency during the maximal velocity phase (p < 0.05). Conclusion: Implementing strategies such as microdosed training load distribution appears to be an effective and efficient alternative for sprint training in team sports such as hockey.


Assuntos
Desempenho Atlético , Hóquei , Corrida , Masculino , Humanos , Esportes de Equipe
5.
Sports Biomech ; 22(7): 834-847, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32567492

RESUMO

This study aimed to compare the effect of three interset rest intervals (1, 3, and 5 minutes) on (I) mean velocity during a resistance training session conducted in a Smith machine with the squat and bench press exercises, and (II) the pre- and post-exercise force-velocity relationship. Fifteen male university students completed three sessions (i.e., Rest 1', Rest 3', and Rest 5') consisting of three sets of five repetitions against the 10RM load during the squat and bench press exercises. The force-velocity relationship (maximal values of force [F0], velocity [v0], and power [Pmax]) was evaluated at the beginning and at the end of each session with the countermovement jump and bench press throw exercises. During training, mean velocity was slower in sets 2 and 3 of the Rest 1' protocol compared to Rest 3' and Rest 5', but no significant differences were present between Rest 3' and Rest 5'. After training, there was a significant decrease in F0 (p = 0.017) and Pmax (p = 0.010), but not in v0 (p = 0.259). These results support the Rest 3' as the most time-efficient protocol, among those analysed, for the maintenance of high mean velocities during training sessions not leading to failure.


Assuntos
Músculo Esquelético , Treinamento Resistido , Humanos , Masculino , Fenômenos Biomecânicos , Levantamento de Peso , Exercício Físico , Treinamento Resistido/métodos , Força Muscular
6.
J Strength Cond Res ; 36(6): 1490-1497, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32569130

RESUMO

ABSTRACT: Cuevas-Aburto, J, Jukic, I, Chirosa-Ríos, LJ, González-Hernández, JM, Janicijevic, D, Barboza-González, P, Guede-Rojas, F, and García-Ramos, A. Effect of traditional, cluster, and rest redistribution set configurations on neuromuscular and perceptual responses during strength-oriented resistance training. J Strength Cond Res 36(6): 1490-1497, 2022-This study aimed to compare the acute effect of traditional (TR), cluster (CL), and rest redistribution (RR) set configurations on neuromuscular and perceptual measures of fatigue. Thirty-one resistance-trained men randomly performed a Control session and 3 experimental sessions consisting of the squat (SQ) and bench press (BP) exercises performed against the 10 repetition maximum load using TR (3 sets of 6 repetitions; 3 minutes of interset rest), CL (3 sets of 6 repetitions; 30 seconds of intraset rest every 2 repetitions; 3 minutes of interset rest), and RR (9 sets of 2 repetitions; 45 seconds of interset rest) set configurations. A significant effect of "set configuration" (p = 0.002) was observed for barbell velocity. The average velocity of the training session was lower for TR compared with CL (% difference = 5.09% in SQ and 5.68% in BP) and RR (% difference = 5.92% in SQ and 2.71% in BP). The 3 set configurations induced comparable decrements in countermovement jump height (% difference from -6.0% to -8.1%) and throwing velocity (% difference from -0.6% to -1.2%). Ratings of perceived exertion (RPE-10) values collected after the sets were higher for TR (SQ: 6.9 ± 0.7 a.u.; BP: 6.8 ± 0.8 a.u.) compared with CL (SQ: 6.2 ± 0.8 a.u.; BP: 6.4 ± 0.7 a.u.) and RR (SQ: 6.2 ± 0.8 a.u.; BP: 6.6 ± 0.9 a.u.), while the session RPE did not differ between the set configurations (p = 0.595). CL and RR set configurations allow for higher velocities and lower RPE values during resistance training sessions not performed to failure in comparison with a TR set configuration.


Assuntos
Treinamento Resistido , Exercício Físico , Humanos , Masculino , Força Muscular/fisiologia , Músculo Esquelético/fisiologia , Postura , Descanso/fisiologia
7.
J Strength Cond Res ; 36(8): 2132-2138, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32796419

RESUMO

ABSTRACT: González-Hernández, JM, Jiménez-Reyes, P, Cerón, JJ, Tvarijonaviciute, A, Llorente-Canterano, FJ, Martínez-Aranda, LM, and García-Ramos, A. Response of muscle damage markers to an accentuated eccentric training protocol: do serum and saliva measurements agree? J Strength Cond Res 36(8): 2132-2138, 2022-This study aimed (a) to examine the acute and delayed responses of 3 muscle damage biomarkers: creatine kinase (CK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) to an accentuated eccentric training protocol in serum, and (b) to explore the changes of these biomarkers in saliva and compare them with serum. Sixteen resistance-trained university students (10 men [age = 26.6 ± 4.8 years, full squat one repetition maximum [1RM] = 103.4 ± 14.4 kg] and 6 women [age = 22.7 ± 1.4 years, full squat estimated 1RM = 68.3 ± 10.5 kg]) completed an accentuated eccentric strength training protocol with the full squat exercise consisting of 8 sets of 10 repetitions against the 120% estimated 1RM load with 5 minutes of interset rest. The activity of muscle damage biomarkers (CK, AST, and LDH) was measured in serum and saliva before training (Pre), 24 hours after training (Post24), and 96 hours after training (Post96). In serum, lower values of the 3 muscle damage markers were observed at Pre compared to Post24 and Post96, whereas no significant differences were observed between Post24 and Post96 for any analyte. In saliva, there was a significant increase in men at Post96 compared with Pre in CK. The correlations between the measurements in serum and saliva ranged from trivial to small ( r = -0.034 to 0.212). These results suggest that the measurement of muscle damage markers in serum and saliva do not provide the same information in the conditions of our study.


Assuntos
Treinamento Resistido , Saliva , Adulto , Biomarcadores , Creatina Quinase , Feminino , Humanos , L-Lactato Desidrogenase , Masculino , Músculo Esquelético/fisiologia , Treinamento Resistido/métodos , Adulto Jovem
8.
PeerJ ; 9: e10942, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33828909

RESUMO

Strength and conditioning specialists commonly deal with the quantification and selection the setting of protocols regarding resistance training intensities. Although the one repetition maximum (1RM) method has been widely used to prescribe exercise intensity, the velocity-based training (VBT) method may enable a more optimal tool for better monitoring and planning of resistance training (RT) programs. The aim of this study was to compare the effects of two RT programs only differing in the training load prescription strategy (adjusting or not daily via VBT) with loads from 50 to 80% 1RM on 1RM, countermovement (CMJ) and sprint. Twenty-four male students with previous experience in RT were randomly assigned to two groups: adjusted loads (AL) (n = 13) and non-adjusted loads (NAL) (n = 11) and carried out an 8-week (16 sessions) RT program. The performance assessment pre- and post-training program included estimated 1RM and full load-velocity profile in the squat exercise; countermovement jump (CMJ); and 20-m sprint (T20). Relative intensity (RI) and mean propulsive velocity attained during each training session (Vsession) was monitored. Subjects in the NAL group trained at a significantly faster Vsession than those in AL (p < 0.001) (0.88-0.91 vs. 0.67-0.68 m/s, with a ∼15% RM gap between groups for the last sessions), and did not achieve the maximum programmed intensity (80% RM). Significant differences were detected in sessions 3-4, showing differences between programmed and performed Vsession and lower RI and velocity loss (VL) for the NAL compared to the AL group (p < 0.05). Although both groups improved 1RM, CMJ and T20, NAL experienced greater and significant changes than AL (28.90 vs.12.70%, 16.10 vs. 7.90% and -1.99 vs. -0.95%, respectively). Load adjustment based on movement velocity is a useful way to control for highly individualised responses to training and improve the implementation of RT programs.

9.
PLoS One ; 16(4): e0249974, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33886639

RESUMO

This study aimed to elucidate whether ballistic, maximal strength and strength-endurance performances are affected by the sex of the evaluator. Sixteen young male handball players attended two testing sessions that only differed in the sex of the evaluators (2 women vs. 2 men). The two sessions were performed in a counterbalanced order. Ballistic performance (countermovement jump height and throwing velocity), maximal strength performance (squat and bench press [BP] one-repetition maximum [1RM]), and strength-endurance performance (number of repetitions-to-failure in BP and average velocity of the set in the squat) were assessed in both sessions. BP 1RM was greater in the presence of women evaluators (p = 0.036, ES = 0.09), whereas no differences were observed for the remaining variables (p ≥ 0.254, ES ≤ 0.19). Low correlations (r median [range] = -0.074 [-0.693, 0.326]) were observed between the different performance tests for the percent differences between both testing sessions. The sex of the evaluators has minimal influence on a number of physical traits in young male handball players when they are tested in the presence of other members of the team, while the low correlations indicate that a higher performance in one test under the presence of women does not imply a higher performance under the presence of women in other performance tests.


Assuntos
Desempenho Atlético/fisiologia , Músculo Esquelético/fisiologia , Resistência Física/fisiologia , Adulto , Estudos Transversais , Feminino , Humanos , Masculino , Força Muscular , Variações Dependentes do Observador , Adulto Jovem
10.
Int J Sports Physiol Perform ; 16(2): 243-249, 2021 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-32957077

RESUMO

PURPOSE: To compare the effects of 2 upper-body strength-training programs differing in set configuration on bench press 1-repetition maximum (BP1RM), bench press throw peak velocity against 30 kg (BPT30), and handball throwing velocity. METHODS: Thirty-five men were randomly assigned to a traditional group (TRG; n = 12), rest redistribution group (RRG; n = 13), or control group (n = 10). The training program was conducted with the bench press exercise and lasted 6 weeks (2 sessions per week): TRG-6 sets × 5 repetitions with 3 minutes of interset rest; RRG-1 set × 30 repetitions with 31 seconds of interrepetition rest. The total rest period (15 min) and load intensity (75% 1RM) were the same for both experimental groups. Subjects performed all repetitions at maximal intended velocity, and the load was adjusted on a daily basis from velocity recordings. RESULTS: A significant time × group interaction was observed for both BP1RM and BPT30 (P < .01) due to the higher values observed at posttest compared with pretest for TRG (effect size [ES] = 0.77) and RRG (ES = 0.56-0.59) but not for the control group (ES ≤ 0.08). The changes in BP1RM and BPT30 did not differ between TRG and RRG (ES = 0.04 and 0.05, respectively). No significant differences in handball throwing velocity were observed between the pretest and posttest (ES = 0.16, 0.22, and 0.02 for TRG, RRG, and control group, respectively). CONCLUSIONS: Resistance-training programs based on not-to-failure traditional and rest redistribution set configurations induce similar changes in BP1RM, BPT30, and handball throwing velocity.


Assuntos
Desempenho Atlético , Força Muscular , Músculo Esquelético/fisiologia , Treinamento Resistido/métodos , Esportes , Humanos , Masculino
11.
J Strength Cond Res ; 35(4): 886-893, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33306589

RESUMO

ABSTRACT: González-Hernández, JM, García-Ramos, A, Colomer-Poveda, D, Tvarijonaviciute, A, Cerón, J, Jiménez-Reyes, P, and Márquez, G. Resistance training to failure vs. not to failure: acute and delayed markers of mechanical, neuromuscular, and biochemical fatigue. J Strength Cond Res 35(4): 886-893, 2021-This study aimed to compare acute and delayed markers of mechanical, neuromuscular, and biochemical fatigue between resistance training sessions leading to or not to failure. Twelve resistance-trained men completed 2 sessions that consisted of 6 sets of the full-squat exercise performed against the 10 repetitions maximum load. In a randomized order, in one session the sets were performed to failure and in the other session the sets were not performed to failure (5 repetitions per set). Mechanical fatigue was quantified through the recording of the mean velocity during all repetitions. The neuromuscular function of the knee extensors was assessed through a maximal voluntary contraction and the twitch interpolation technique before training, immediately after each set, and 1, 24, and 48 hours post-training. Serum creatine kinase (CK) and aspartate aminotransferase (AST) were measured before training and 1, 24, and 48 hours post-training to infer muscle damage. Alpha was set at a level of 0.05. A higher velocity loss between sets was observed during the failure protocol (-21.7%) compared with the nonfailure protocol (-3.5%). The markers of peripheral fatigue were generally higher and long lasting for the failure protocol. However, the central fatigue assessed by the voluntary activation was comparable for both protocols and remained depressed up to 48 hours post-training. The concentrations of CK and AST were higher after the failure protocol revealing higher muscle damage compared with the nonfailure protocol. These results support the nonfailure protocol to reduce peripheral fatigue and muscle damage, whereas the central fatigue does not seem to be affected by the set configuration.


Assuntos
Treinamento Resistido , Exercício Físico , Humanos , Masculino , Fadiga Muscular , Força Muscular , Músculo Esquelético , Postura
12.
Phys Ther Sport ; 44: 136-142, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32506036

RESUMO

OBJECTIVE: To analyze the concurrent validity and reliability of an iPhone application for assessing range of motion (ROM) and joint position sense (JPS) in ankle and knee joints. DESIGN: Cross-sectional study. SETTING: Sport laboratory. PARTICIPANTS: Twenty healthy and physically active volunteers. INTERVENTIONS: All participants performed a ROM and a JPS test in ankle and knee joints, which were simultaneously evaluated with photo-analysis and the iPhone application. MAIN OUTCOMES MEASURES: A total of 80 angles were obtained with the iPhone app and the photo-analysis and compared for concurrent validity. Reliability was evaluated through re-scoring of images with the iPhone app by two different testers. RESULTS: Very high correlation was observed between both methods for ankle and knee ROM and knee JPS (r > 0.90), and high correlation for ankle JPS (r = 0.71-0.90), while Bland-Altman plots showed absolute agreement for all the variables. Inter- and intra-tester reliability was perfect for all the variables (ICC > 0.81), except for the inter-tester reliability of ankle JPS, which was substantial (ICC = 0.61-0.81). CONCLUSIONS: This new iPhone application is valid and reliable for measuring ankle and knee ROM and JPS, although special attention is needed during ankle evaluation to avoid errors.


Assuntos
Articulação do Tornozelo/fisiologia , Articulação do Joelho/fisiologia , Propriocepção/fisiologia , Amplitude de Movimento Articular/fisiologia , Smartphone , Adulto , Estudos Transversais , Feminino , Voluntários Saudáveis , Humanos , Masculino , Reprodutibilidade dos Testes
13.
PeerJ ; 8: e8760, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32201649

RESUMO

BACKGROUND: This study aimed to determine the reliability of the velocity achieved during the last repetition of sets to failure (V last) and the association of V last with the velocity of the 1-repetition maximum (V 1RM) during the paused and touch-and-go bench press (BP) exercises performed in a Smith machine. METHODS: A total of 96 healthy men participated in this study that consisted of two testing sessions. A single BP variant (paused BP or touch-and-go BP) was evaluated on each session in a randomized order. Each session consisted of an incremental loading test until reaching the 1RM, followed by two sets of repetitions to failure against a load ranging from 75% to 90% of 1RM. RESULTS: The reliability of V last was unacceptable for both BP variants (CV > 18.3%, ICC < 0.60). The correlations between V 1RM and V last were small for the paused BP (r = 0.18) and moderate for the touch-and-go BP (r = 0.37). CONCLUSIONS: Although these results suggest that V last could be a better indicator of the minimal velocity threshold than V 1RM, the low reliability of V last and the similar values of V last for both BP variants suggest that a standard V 1RM should be used to estimate the 1RM from the individualized load-velocity relationship.

14.
J Sports Sci ; 38(7): 759-766, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32100628

RESUMO

This study aimed to compare the reliability and magnitude of velocity variables between 3 variants of the bench press (BP) exercise in participants with and without BP training experience. Thirty males, 15 with and 15 without BP experience, randomly performed 3 variants of the BP on separate sessions: (I) concentric-only, (II) fast-eccentric and (III) controlled-eccentric. The mean velocity (MV) and maximum velocity (Vmax) of the concentric phase were collected against 3 loads (≈30%1RM, 50%1RM, and 75%1RM) with a linear velocity transducer. Reliability was high regardless of the variable, BP variant, and load (coefficient of variation [CV] ≤ 4.47%, intraclass correlation coefficient [ICC] ≥ 0.87). The comparison of the CVs suggested a higher reliability for the fast-eccentric BP (8 out of 12 comparisons), followed by the concentric-only BP (5 out of 12 comparisons), and finally the controlled-eccentric BP (never provided a higher reliability). No differences in reliability were observed between experienced (CV ≤ 4.71%; ICC ≥ 0.79) and non-experienced (CV ≤ 6.29%; ICC ≥ 0.76) participants. The fast-eccentric BP provided the highest MV (p < 0.05) and no differences were observed for Vmax. These results support the assessment of movement velocity during the fast-eccentric BP even in participants without experience.


Assuntos
Treinamento Resistido/métodos , Extremidade Superior/fisiologia , Levantamento de Peso/fisiologia , Adolescente , Estudos Cross-Over , Humanos , Masculino , Movimento/fisiologia , Força Muscular/fisiologia , Análise e Desempenho de Tarefas , Adulto Jovem
15.
J Proteomics ; 214: 103629, 2020 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-31881350

RESUMO

The aim of the present study was to evaluate the differences in salivary proteome at rest and in response to an acute exercise in men and women. For this, unstimulated whole saliva samples in rest and after a bout resistance exercise leading to failure (ELF) of both men and women (n = 5 for each sex) were subjected to isobaric Tandem Mass Tags (TMT) labelling followed by LC-MS/MS. A total of 274 proteins were identified and met the inclusion criteria. 16 proteins were modulated for the interaction sex*exercise, 6 were modulated because of the exercise, and 65 were differentially expressed between men and women at rest. In conclusion, these results indicate sex-related differences in the salivary proteome at rest and after an acute exercise, pointing out possible candidate biomarkers for sports performance and allowing further knowledge of the physiological processes occurring during ELF. SIGNIFICANCE: The present study describes for the first time the changes that occur in salivary proteome detected by TMT-based proteomics in response to an acute exercise and the differences in these changes depending on sex. Of the 274 protein identified, the 87 differentially expressed proteins and their related pathways were discussed, focusing on the sex- and exercise-related differences in the salivary proteome.


Assuntos
Proteoma , Espectrometria de Massas em Tandem , Cromatografia Líquida , Exercício Físico , Feminino , Humanos , Masculino , Projetos Piloto , Saliva , Proteínas e Peptídeos Salivares
16.
J Strength Cond Res ; 34(8): 2220-2226, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29952872

RESUMO

Párraga-Montilla, JA, García-Ramos, A, Castaño-Zambudio, A, Capelo-Ramírez, F, González-Hernández, JM, Cordero-Rodríguez, Y, and Jiménez-Reyes, P. Acute and delayed effects of a resistance training session leading to muscular failure on mechanical, metabolic, and perceptual responses. J Strength Cond Res 34(8): 2220-2226, 2020-This study explored the acute and delayed (24 and 48 hours after exercise) effects of a resistance training session leading to muscular failure. Eleven resistance-trained men completed a training session consisting on 3 sets of repetitions to failure during the back-squat exercise performed at the maximum possible speed with a load equivalent to a mean propulsive velocity (MPV) of 1 m·s (≈60% of 1 repetition maximum). A number of mechanical (number of repetitions and starting MPV of the set, MPV achieved against the 1MPV load, countermovement jump [CMJ] height, and handgrip strength), metabolic (lactate, uric acid, and ammonia concentrations), and perceptual (OMNI-RES perceived exertion) variables were measured. The results revealed (a) a decrease of 38.7% in set 2 and 54.7% in set 3 of the number of repetitions performed compared with the first set (p < 0.05), (b) a reduction in the MPV of the repetitions and an increase in lactate concentration and OMNI-RES values with the succession of sets (p < 0.05), (c) comparable decrements in CMJ height after the 3 sets (25-32%), (d) a decrease in CMJ height (p < 0.05; 6.7-7.9%) and in the MPV attained against the 1MPV load (p < 0.05; 13-14%) after 24 and 48 hours of completing the training session, but no significant changes were observed in handgrip strength (p > 0.05; <2%), and (e) uric acid and ammonia concentrations above the basal levels (p < 0.05). The large decrements in mechanical performance together with the high metabolic stress discourage the frequent use of resistance training sessions leading to muscular failure.


Assuntos
Fadiga Muscular/fisiologia , Força Muscular/fisiologia , Músculo Esquelético/fisiologia , Treinamento Resistido/métodos , Adolescente , Adulto , Amônia/sangue , Força da Mão , Humanos , Ácido Láctico/sangue , Masculino , Postura , Fatores de Tempo , Ácido Úrico/sangue , Adulto Jovem
17.
J Strength Cond Res ; 34(6): 1581-1590, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28700515

RESUMO

González-Hernández, JM, García-Ramos, A, Castaño-Zambudio, A, Capelo-Ramírez, F, Marquez, G, Boullosa, D, and Jiménez-Reyes, P. Mechanical, metabolic, and perceptual acute responses to different set configurations in full squat. J Strength Cond Res 34(6): 1581-1590, 2020-This study aimed to compare mechanical, metabolic, and perceptual responses between 2 traditional (TR) and 4 cluster (CL) set configurations. In a counterbalanced randomized order, 11 men were tested with the following protocols in separate sessions (sets × repetitions [interrepetition rest]): TR1: 3 × 10 [0 seconds]; TR2: 6 × 5 [0 seconds]; CL1: 3 × 10 [10 seconds]; CL2: 3 × 10 [15 seconds]; CL3: 3 × 10 [30 seconds]; CL4: 1 × 30 [15 seconds]. The exercise (full squat), number of repetitions (30), interset rest (5 minutes), and resistance applied (10 repetition maximum) was the same for all set configurations. Mechanical fatigue was quantified by measuring the mean propulsive velocity during each repetition and the change in countermovement jump height observed after each set and after the whole training session. Metabolic and perceptual fatigue were assessed via the blood lactate concentration and the OMNI perceived exertion scale measured after each training set, respectively. The mechanical, metabolic, and perceptual measures of fatigue were always significantly higher for the TR1 set configuration. The 2 set configurations that most minimized the mechanical measures of fatigue were CL2 and CL3. Perceived fatigue did not differ between the TR2, CL1, CL2, and CL3 set configurations. The lowest lactate concentration was observed in the CL3 set configuration. Therefore, both the CL2 and CL3 set configurations can be recommended because they maximize mechanical performance. However, the CL2 set configuration presents 2 main advantages with respect to CL3 (a): it reduces training session duration, and (b) it promotes higher metabolic stress, which, to some extent, may be beneficial for inducing muscle strength and hypertrophy gains.


Assuntos
Exercício Físico/fisiologia , Força Muscular/fisiologia , Percepção , Treinamento Resistido/métodos , Adulto , Fenômenos Biomecânicos , Humanos , Ácido Láctico/sangue , Masculino , Fadiga Muscular/fisiologia , Postura , Descanso/fisiologia , Adulto Jovem
18.
J Strength Cond Res ; 34(3): 663-670, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29076963

RESUMO

García-Ramos, A, González-Hernández, JM, Baños-Pelegrín, E, Castaño-Zambudio, A, Capelo-Ramírez, F, Boullosa, D, Haff, GG, and Jiménez-Reyes, P. Mechanical and metabolic responses to traditional and cluster set configurations in the bench press exercise. J Strength Cond Res 34(3): 663-670, 2020-This study aimed to compare mechanical and metabolic responses between traditional (TR) and cluster (CL) set configurations in the bench press exercise. In a counterbalanced randomized order, 10 men were tested with the following protocols (sets × repetitions [inter-repetition rest]): TR1: 3 × 10 (0-second), TR2: 6 × 5 (0-second), CL5: 3 × 10 (5-second), CL10: 3 × 10 (10-second), and CL15: 3 × 10 (15-second). The number of repetitions (30), interset rest (5 minutes), and resistance applied (10 repetition maximum) were the same for all set configurations. Movement velocity and blood lactate concentration were used to assess the mechanical and metabolic responses, respectively. The comparison of the first and last set of the training session revealed a significant decrease in movement velocity for TR1 (Effect size [ES]: -0.92), CL10 (ES: -0.85), and CL15 (ES: -1.08) (but not for TR2 [ES: -0.38] and CL5 [ES: -0.37]); while blood lactate concentration was significantly increased for TR1 (ES: 1.11), TR2 (ES: 0.90), and CL5 (ES: 1.12) (but not for CL10 [ES: 0.03] and CL15 [ES: -0.43]). Based on velocity loss, set configurations were ranked as follows: TR1 (-39.3 ± 7.3%) > CL5 (-20.2 ± 14.7%) > CL10 (-12.9 ± 4.9%), TR2 (-10.3 ± 5.3%), and CL15 (-10.0 ± 2.3%). The set configurations were ranked as follows based on the lactate concentration: TR1 (7.9 ± 1.1 mmol·L) > CL5 (5.8 ± 0.9 mmol·L) > TR2 (4.2 ± 0.7 mmol·L) > CL10 (3.5 ± 0.4 mmol·L) and CL15 (3.4 ± 0.7 mmol·L). These results support the use of TR2, CL10, and CL15 for the maintenance of high mechanical outputs, while CL10 and CL15 produce less metabolic stress than TR2.


Assuntos
Músculo Esquelético/fisiologia , Treinamento Resistido/métodos , Levantamento de Peso/fisiologia , Adulto , Exercício Físico , Humanos , Ácido Láctico/sangue , Masculino , Descanso/fisiologia , Adulto Jovem
19.
PeerJ ; 7: e7533, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31497399

RESUMO

BACKGROUND: Movement velocity has been proposed as an effective tool to prescribe the load during resistance training in young healthy adults. This study aimed to elucidate whether movement velocity could also be used to estimate the relative load (i.e., % of the one-repetition maximum (1RM)) in older women. METHODS: A total of 22 older women (age = 68.2 ± 3.6 years, bench press 1RM = 22.3 ± 4.7 kg, leg press 1RM = 114.6 ± 15.9 kg) performed an incremental loading test during the free-weight bench press and the leg press exercises on two separate sessions. The mean velocity (MV) was collected with a linear position transducer. RESULTS: A strong linear relationship between MV and the relative load was observed for the bench press (%1RM = -130.4 MV + 119.3; r 2 = 0.827, standard error of the estimate (SEE) = 6.10%1RM, p < 0.001) and leg press exercises (%1RM = -158.3 MV + 131.4; r 2 = 0.913, SEE = 5.63%1RM, p < 0.001). No significant differences were observed between the bench press and leg press exercises for the MV attained against light-medium relative loads (≤70%1RM), while the MV associated with heavy loads (≥80%1RM) was significantly higher for the leg press. CONCLUSIONS: These results suggest that the monitoring of MV could be useful to prescribe the loads during resistance training in older women. However, it should be noted that the MV associated with a given %1RM is significantly lower in older women compared to young healthy individuals.

20.
Antioxidants (Basel) ; 8(9)2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31514287

RESUMO

Uric acid (UA) is the most abundant antioxidant compound in saliva and one of the most sensitive biomarkers for detecting changes in the oxidative status of the organism. The aim of this study was to evaluate the effect of: (i) different methods of saliva sampling and (ii) the correction by salivary flow or total protein on UA concentrations in saliva. Paired saliva (collected by two different methods, passive drooling and using Salivette cotton rolls) and serum samples were obtained from 12 healthy men after the performance of two resistance training exercises of different level of effort that can produce different concentrations in UA in saliva. There were no significant differences between values of uric acid in saliva using Salivette and passive drool. Correlations between UA in serum and saliva and increases in UA in saliva after exercise were detected when saliva samples were obtained by passive drool and Salivette and were not corrected by salivary flow or total protein concentration. Therefore for UA measurements in saliva it would not be recommended to normalize the results by salivary flow or protein concentration. This study highlights the importance of choosing an adequate sampling method selection as well as the expression of results when analytes are measured in saliva.

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